1-(Pyrrolidine-1-carbonyl)pyridin-1-ium chloride | 139486-47-8

• 分子结构分类: 有机化合物 - 有机杂环化合物 - 吡咯烷类
• 英文名称: 1-(Pyrrolidine-1-carbonyl)pyridin-1-ium chloride
• 英文别名: pyridin-1-ium-1-yl(pyrrolidin-1-yl)methanone;chloride
• CAS: 139486-47-8
• 化学式: C10H13ClN2O
• 分子量: 212.67
• InChiKey: TTWCDFJTYXKJGU-UHFFFAOYSA-M

计算性质

• 辛醇/水分配系数(LogP)：
  -1.96
• 重原子数：
  14
• 可旋转键数：
  0
• 环数：
  2.0
• sp3杂化的碳原子比例：
  0.4
• 拓扑面积：
  24.2
• 氢给体数：
  0
• 氢受体数：
  2

文献信息

• Immunoreagent composition, test kit and rapid assay for human chorionic gonadotropin
  申请人：CLINICAL DIAGNOSTIC SYSTEMS, INC.

  公开号：EP0456308A1

  公开（公告）日：1991-11-13

  A rapid immunometric assay for human chorionic gonadotropin (hCG), commonly used for detection of pregnancy in human females, has been developed using a specific wash solution and conjugate level which reduces background and improves sensitivity. The assay can be carried out within two minutes using a microporous membrane to capture the "sandwich" complex of antigen and two antibodies, one of which is located on the membrane. Greater sensitivity at low levels of hCG have been demonstrated so the assay is useful as an early indicator of pregnancy. An immunoreagent composition and test kit are provided for use in the assay.

  人类绒毛膜促性腺激素（hCG）通常用于检测人类女性是否怀孕，我们开发出了一种快速免疫测定法，该方法采用特定的洗涤液和共轭物水平，可降低本底，提高灵敏度。使用微孔膜捕获抗原和两种抗体的 "三明治 "复合物（其中一种抗体位于膜上），可在两分钟内完成检测。实验证明，当 hCG 含量较低时，检测灵敏度更高，因此该检测方法可作为怀孕的早期指标。我们提供了用于检测的免疫试剂组合物和检测试剂盒。
• Biologically active reagents prepared from carboxy-containing polymer, analytical element and methods of use
  申请人：Johnson & Johnson Clinical Diagnostics, Inc.

  公开号：EP0462644A1

  公开（公告）日：1991-12-27

  Biologically active reagents are prepared from particles of copolymers having highly reactive carboxy or equivalent groups. The reagents are prepared by covalently attaching biologically active substances, for example antibodies, to the particles, directly or indirectly through highly reactive carboxy groups on the particle surface. These reagents are used to advantage in analytical elements, methods for the detection of specific binding ligands (such as immunological species) and immunoassays, and in purification methods as affinity chromatography reagents.

  生物活性试剂由具有高活性羧基或等效基团的共聚物颗粒制备而成。制备试剂的方法是将生物活性物质（如抗体）直接或间接地通过颗粒表面的高活性羧基共价连接到颗粒上。这些试剂可用于分析元件、检测特定结合配体（如免疫物种）的方法和免疫测定，也可作为亲和层析试剂用于纯化方法。
• Method of preparing biologically active reagents from succinimide-containing polymers, analytical element and methods of use
  申请人：Johnson & Johnson Clinical Diagnostics, Inc.

  公开号：EP0496473A2

  公开（公告）日：1992-07-29

  Biologically active reagents are prepared from particles of copolymers having highly active succinimide groups. The reagents are prepared by covalently attaching biologically active substances, for example antibodies, to the particles, directly or indirectly through amide groups by displacement of highly active succinimide groups on the particle surface. These reagents are used to advantage in analytical elements, methods for the detection of specific binding ligands (such as immunological species) and immunoassays, and in purification methods such as affinity chromatography.

  生物活性试剂由具有高活性琥珀酰亚胺基团的共聚物颗粒制备而成。这些试剂的制备方法是通过酰胺基团置换颗粒表面的高活性琥珀酰亚胺基团，将生物活性物质（如抗体）直接或间接地共价连接到颗粒上。这些试剂在分析元件、特定结合配体（如免疫物种）的检测方法、免疫测定以及亲和层析等纯化方法中得到了广泛应用。
• Wash composition containing signal stop reagent, test kit and method of use with peroxidase-labeled specific binding ligand
  申请人：Johnson & Johnson Clinical Diagnostics, Inc.

  公开号：EP0537825A1

  公开（公告）日：1993-04-21

  A buffered composition containing a nonionic or anionic surfactant, and a certain amount of a hydroxamic acid (or salt thereof) or an acyl hydrazine can be used as a wash composition in specific binding assays. Such assays include the use of a peroxidase labeled specific binding reactant to form a detectable peroxidase-labeled specific binding complex. The buffered composition and labeled reactant can be supplied in a diagnostic test kit. The hydroxamic acid or acyl hydrazine can be represented by the structure(I):         R-CO-NH-R' wherein R is aryl or 6 to 10 carbon atoms in the aromatic nucleus, alkyl of 1 to 7 carbon atoms, cycloalkyl of 5 to 10 carbon atoms in the ring, or heterocyclyl of 5 to 10 atoms in the ring, at least one of which is an oxygen, nitrogen or sulfur atom, and R' is hydroxy or amino.

  含有非离子或阴离子表面活性剂和一定量的羟肟酸（或其盐）或酰基肼的缓冲组合物可用作特异性结合试验中的洗涤组合物。此类检测包括使用过氧化物酶标记的特异性结合反应物，以形成可检测的过氧化物酶标记的特异性结合复合物。缓冲组合物和标记反应物可在诊断检测试剂盒中提供。羟肟酸或酰基肼可由结构(I)表示： R-CO-NH-R' 其中 R 是芳基或芳香核中 6 至 10 个碳原子的芳基、1 至 7 个碳原子的烷基、环中 5 至 10 个碳原子的环烷基或环中 5 至 10 个原子的杂环烷基，其中至少一个是氧、氮或硫原子，R'是羟基或氨基。
• Method and kit for attaching compounds to carboxylated substrates using uronium salt
  申请人：Johnson & Johnson Clinical Diagnostics, Inc.

  公开号：EP0569086A2

  公开（公告）日：1993-11-10

  Certain uronium salts are useful for attaching biological or chemical compounds having a reactive amino or sulfhydryl group to a carboxylated substrate, such as carboxylated particles. These salts are 2-(N-heterocyclyloxy)uronium salts which activate reactive carboxy groups for reaction with the amino or sulfhydryl groups. The resulting reagents are useful in various analytical, diagnostic and medical applications, or can be used as conjugates in various immunoassays.

  某些脲盐可用于将具有活性氨基或巯基的生物或化学物质附着到羧化基质（如羧化微粒）上。这些盐是 2-（N-杂环氧基）脲盐，可激活活性羧基与氨基或巯基反应。由此产生的试剂可用于各种分析、诊断和医疗应用，或在各种免疫测定中用作共轭物。