A substantially enzymatically pure hydrolase is provided which is secreted by and isolatable from Pseudomonas putida ATCC 52552. Cloning the gene expressing the hydrolase into a suitable expression vector and culturing, such as fermenting the E. coli strain JM101 harboring a plasmid designated pSNtacII, has been found to provide surprisingly high yields of the hydrolase.
本发明提供了一种酶纯度很高的
水解酶,它由假单胞菌(Pseudomonas putida A
TCC 52552)分泌,并可从假单胞菌(Pseudomonas putida A
TCC 52552)中分离出来。已发现将表达
水解酶的
基因克隆到合适的表达载体中并进行培养,如发酵大肠杆菌菌株 JM101(该菌株含有名为 pSNtacII 的质粒),可获得高产率的
水解酶。