2-(4-fluorophenylmethyl)-6-(4-hydroxyphenyl)-8-benzyl-3,7-dihydroimidazo[1,2-a]pyrazine-3-one | 123437-16-1

• 分子结构分类: 有机化合物 - 有机杂环化合物 - 咪唑并吡嗪类
• 英文名称: 2-(4-fluorophenylmethyl)-6-(4-hydroxyphenyl)-8-benzyl-3,7-dihydroimidazo[1,2-a]pyrazine-3-one
• 英文别名: 8-benzyl-2-[(4-fluorophenyl)methyl]-6-(4-hydroxyphenyl)-7H-imidazo[1,2-a]pyrazin-3-one
• CAS: 123437-16-1
• 化学式: C₂₆H₂₀FN₃O₂
• 分子量: 425.462
• InChiKey: VEADHSBKGZIWMA-UHFFFAOYSA-N

物化性质

• 熔点：
  >200℃
• 溶解度：
  溶于乙醇、甲醇
• 最大波长(λmax)：
  437nm

计算性质

• 辛醇/水分配系数(LogP)：
  4.69
• 重原子数：
  32.0
• 可旋转键数：
  5.0
• 环数：
  5.0
• sp3杂化的碳原子比例：
  0.08
• 拓扑面积：
  70.91
• 氢给体数：
  2.0
• 氢受体数：
  4.0

安全信息

• 危险品标志：
  Xi
• 安全说明：
  S26,S36
• 危险类别码：
  R36/37/38

反应信息

• 作为反应物：
  描述：

  2-(4-fluorophenylmethyl)-6-(4-hydroxyphenyl)-8-benzyl-3,7-dihydroimidazo[1,2-a]pyrazine-3-one 在 manganese(IV) oxide 作用下， 以 甲醇 、 乙醚 、 乙醇 、 二氯甲烷 为溶剂， 反应 4.0h， 生成
  参考文献：
  名称：

  19F-Dehydrocoelenterazine as probe to investigate the active site of symplectin

  摘要：

  Fluorinated dehydrocoelenterazines (F-DCTs) were synthesized to study molecular mechanisms of symplectin; a photoprotein of luminous squid Symplectoteuthis oualaniensis L. F-DCTs reacted with dithiothreitol and glutathione under neutral conditions to give the stable chromophores as symplectin model. Reconstructed symplectin was also obtained by addition of F-DCTs into apo-symplectin, and showed bioluminescence to emit 50-65% amount of light as natural symplectin. The structure of the chromophores was determined by F-19 NMR, Q-TOF-MS, and MS/MS analyses. Sequencing of the chromopeptides of symplectin models prepared from F-DCTs and thiol compounds was accomplished by ESI-Q-TOF-MS/MS analysis. (C) 2002 Elsevier Science Ltd. All rights reserved.

  DOI：

  10.1016/s0040-4020(02)00100-x
• 作为产物：
  描述：

  对氟苯乙酰氯 在 盐酸 、 sodium hydroxide 、 硫酸 、 氢溴酸 作用下， 以 1,4-二氧六环 、 乙醚 、 二氯甲烷 、 水 、 溶剂黄146 为溶剂， 反应 6.5h， 生成 2-(4-fluorophenylmethyl)-6-(4-hydroxyphenyl)-8-benzyl-3,7-dihydroimidazo[1,2-a]pyrazine-3-one
  参考文献：
  名称：

  19F-Dehydrocoelenterazine as probe to investigate the active site of symplectin

  摘要：

  Fluorinated dehydrocoelenterazines (F-DCTs) were synthesized to study molecular mechanisms of symplectin; a photoprotein of luminous squid Symplectoteuthis oualaniensis L. F-DCTs reacted with dithiothreitol and glutathione under neutral conditions to give the stable chromophores as symplectin model. Reconstructed symplectin was also obtained by addition of F-DCTs into apo-symplectin, and showed bioluminescence to emit 50-65% amount of light as natural symplectin. The structure of the chromophores was determined by F-19 NMR, Q-TOF-MS, and MS/MS analyses. Sequencing of the chromopeptides of symplectin models prepared from F-DCTs and thiol compounds was accomplished by ESI-Q-TOF-MS/MS analysis. (C) 2002 Elsevier Science Ltd. All rights reserved.

  DOI：

  10.1016/s0040-4020(02)00100-x

文献信息

• COELENTERAZINE ANALOGUES
  申请人：PROMEGA CORPORATION

  公开号：US20200062766A1

  公开（公告）日：2020-02-27

  Described are coelenterazine analogues, methods for making the analogues, kits comprising the analogues, and methods of using the compounds for the detection of luminescence in luciferase-based assays.

  本文描述了腔肠动物素类似物、制备该类似物的方法、包含该类似物的试剂盒以及使用该化合物检测荧光素酶基础测定法中发光的方法。
• Synthesis of coelenterazine
  申请人：International Paper Company

  公开号：US11008326B2

  公开（公告）日：2021-05-18

  Disclosed herein are synthesis methods for coelenterazine. Also disclosed are articles including the coelenterazine and coelenterazine derivatives. Representative absorbent articles include disposable diapers and adult incontinence products.

  本文公开了腔肠素的合成方法。还公开了包括腔肠素和腔肠素衍生物的物品。具有代表性的吸收物品包括一次性尿布和成人失禁用品。
• Full length kinase activity-conformation reporter
  申请人：UNIVERSITAT INNSBRUCK

  公开号：US11237173B2

  公开（公告）日：2022-02-01

  The present invention provides a reporter for a protein fragment complementation assay characterized in that the reporter is a fused protein comprising a first fragment, a second fragment and a protein kinase sequence section, wherein the first fragment and the second fragment are derived from different sections of the same split protein, and wherein the protein kinase sequence section intervenes between the first fragment and the second fragment and wherein the kinase sequence section comprises a kinase domain sequence and one or more regulatory sequence(s). Further the invention provides polynucleotides and cells encoding for the reporter as well as methods of conducting a protein fragment complementation assay with the reporter according to the invention.

  本发明提供了一种用于蛋白质片段互补检测的报告基因，其特征在于该报告基因是一种融合蛋白，包括第一片段、第二片段和蛋白质激酶序列部分，其中第一片段和第二片段来自同一分裂蛋白的不同部分，蛋白质激酶序列部分介于第一片段和第二片段之间，其中激酶序列部分包括激酶结构域序列和一个或多个调控序列。此外，本发明还提供了编码报告基因的多核苷酸和细胞，以及用本发明报告基因进行蛋白质片段互补检测的方法。
• FULL LENGTH KINASE ACTIVITY-CONFORMATION REPORTER
  申请人：Universität Innsbruck

  公开号：EP3519830A1

  公开（公告）日：2019-08-07
• SECRETED LUCIFERASE MLUC7 AND USE THEREOF
  申请人：Golz Stefan

  公开号：US20100105090A1

  公开（公告）日：2010-04-29

  The invention relates to the nucleotide and amino acid sequences and to the activity and use of the secreted MLuc7 luciferase.