5-carboxy-2-[5-(5-carboxy-1,3,3-trimethyl-2,3-dihydro-1H-indol-2-ylidene)pent-1,3-dienyl]-1,3,3-trimethyl-3H-indolium iodide | 1103482-91-2

• 分子结构分类: 有机化合物 - 有机杂环化合物 - 吲哚及其衍生物
• 英文名称: 5-carboxy-2-[5-(5-carboxy-1,3,3-trimethyl-2,3-dihydro-1H-indol-2-ylidene)pent-1,3-dienyl]-1,3,3-trimethyl-3H-indolium iodide
• CAS: 1103482-91-2
• 化学式: C₂₉H₃₁N₂O₄*I
• 分子量: 598.481
• InChiKey: ZRXRUOUDIFTRHN-UHFFFAOYSA-N

计算性质

• 辛醇/水分配系数(LogP)：
  2.52
• 重原子数：
  36.0
• 可旋转键数：
  5.0
• 环数：
  4.0
• sp3杂化的碳原子比例：
  0.28
• 拓扑面积：
  80.85
• 氢给体数：
  2.0
• 氢受体数：
  3.0

反应信息

• 作为反应物：
  描述：

  5-carboxy-2-[5-(5-carboxy-1,3,3-trimethyl-2,3-dihydro-1H-indol-2-ylidene)pent-1,3-dienyl]-1,3,3-trimethyl-3H-indolium iodide 、 苯胺 在 4-二甲氨基吡啶 、 N,N'-二环己基碳二亚胺 作用下， 以 乙腈 为溶剂， 反应 19.5h， 以40%的产率得到1,3,3-trimethyl-5-(phenylcarbamoyl)-2-(5-(1,3,3-trimethyl-5-(phenylcarbamoyl)indolin-2-ylidene)penta-1,3-dien-1-yl)-3H-indol-1-ium iodide
  参考文献：
  名称：

  Synthesis and spectral properties of polymethine-cyanine dye–nitroxide radical hybrid compounds for use as fluorescence probes to monitor reducing species and radicals

  摘要：

  Various hybrid compounds comprised of two types of nitroxide radicals and either a pentamethine (Cy5) or trimethine cyanine (Cy3) were synthesized. The nitroxide radicals were linked either via an ester-bond to one or two N-alkyl carboxyl-terminated groups of Cy5, or via two amido-bonds (aminocarbonyl or carborylamino group) to the 5-position of the indolenine moieties of Cy5 and Cy3. Changes in fluorescence and ESR intensities of the hybrid compounds were measured before and after addition of Na ascorbate in PBS (pH 7.0) to reduce the radicals. Among the hybrid compounds synthesized, those that linked the nitroxide radicals via an aminocarbonyl residue at the 5-position of the indolenine moieties on Cy5 and Cy3 exhibited a 1.8- and 5.1-fold increase in fluorescence intensity with the reduction of the nitroxide segment by the addition of Na ascorbate, respectively. In contrast, fluorescence intensity was not enhanced in the other hybrid compounds. Thus, the hybrid compounds which exhibited an increase in fluorescent intensity with radical reduction can be used in the quantitative measurement of reducing species such as Fe(2+) and ascorbic acid, and hydroxyl radicals. Because these hybrid compounds have the advantage of fluorescing at longer wavelengths-661 (Cy5) or 568 (Cy3) nm, respectively. they can be used to measure radical-reducing species or radicals either in solution or in vivo. (C) 2008 Elsevier B.V. All rights reserved.

  DOI：

  10.1016/j.saa.2008.07.045
• 作为产物：
  描述：

  1,1,3,3-四乙氧基丙烷 、 5-carboxy-1,2,3,3-tetramethyl-3H-indol-1-ium iodide 在 吡啶 作用下， 反应 10.5h， 以66%的产率得到5-carboxy-2-[5-(5-carboxy-1,3,3-trimethyl-2,3-dihydro-1H-indol-2-ylidene)pent-1,3-dienyl]-1,3,3-trimethyl-3H-indolium iodide
  参考文献：
  名称：

  Synthesis and spectral properties of polymethine-cyanine dye–nitroxide radical hybrid compounds for use as fluorescence probes to monitor reducing species and radicals

  摘要：

  Various hybrid compounds comprised of two types of nitroxide radicals and either a pentamethine (Cy5) or trimethine cyanine (Cy3) were synthesized. The nitroxide radicals were linked either via an ester-bond to one or two N-alkyl carboxyl-terminated groups of Cy5, or via two amido-bonds (aminocarbonyl or carborylamino group) to the 5-position of the indolenine moieties of Cy5 and Cy3. Changes in fluorescence and ESR intensities of the hybrid compounds were measured before and after addition of Na ascorbate in PBS (pH 7.0) to reduce the radicals. Among the hybrid compounds synthesized, those that linked the nitroxide radicals via an aminocarbonyl residue at the 5-position of the indolenine moieties on Cy5 and Cy3 exhibited a 1.8- and 5.1-fold increase in fluorescence intensity with the reduction of the nitroxide segment by the addition of Na ascorbate, respectively. In contrast, fluorescence intensity was not enhanced in the other hybrid compounds. Thus, the hybrid compounds which exhibited an increase in fluorescent intensity with radical reduction can be used in the quantitative measurement of reducing species such as Fe(2+) and ascorbic acid, and hydroxyl radicals. Because these hybrid compounds have the advantage of fluorescing at longer wavelengths-661 (Cy5) or 568 (Cy3) nm, respectively. they can be used to measure radical-reducing species or radicals either in solution or in vivo. (C) 2008 Elsevier B.V. All rights reserved.

  DOI：

  10.1016/j.saa.2008.07.045