Ethylenediamine mandelate | 17140-83-9

• 分子结构分类: 有机化合物 - 苯类化合物 - 苯和取代衍生物
• 英文名称: Ethylenediamine mandelate
• 英文别名: ethane-1,2-diamine;2-hydroxy-2-phenylacetic acid
• CAS: 17140-83-9
• 化学式: C10H16N2O3
• 分子量: 212.25
• InChiKey: MXESQYGXOLPAJN-UHFFFAOYSA-N

计算性质

• 辛醇/水分配系数(LogP)：
  -0.29
• 重原子数：
  15
• 可旋转键数：
  3
• 环数：
  1.0
• sp3杂化的碳原子比例：
  0.3
• 拓扑面积：
  110
• 氢给体数：
  4
• 氢受体数：
  5

文献信息

• CHROMOGENIC PEROXIDASE SUBSTRATES
  申请人：Dako Denmark A/S

  公开号：US20170175178A1

  公开（公告）日：2017-06-22

  Chromogenic conjugates for color-based detection of targets are described. The conjugates comprise a chromogenic moiety such as rhodamine, rhodol or fluorescein. The chromogenic moiety is linked to a peroxidase substrate. The chromogenic conjugates can be used in immunohistochemical analysis and in situ hybridization. The conjugates can be used to detect 1, 2, 3 or more targets in a sample by color.

  描述了用于基于颜色的目标检测的色素结合物。这些结合物包含一个色素部分，例如罗丹明、罗丹醇或荧光素。色素部分与过氧化物酶底物相连。色素结合物可用于免疫组织化学分析和原位杂交。这些结合物可以通过颜色检测样品中的1个、2个、3个或更多目标。
• Rapid and sensitive method for detection of biological targets
  申请人：Dako Denmark A/S

  公开号：US10168335B2

  公开（公告）日：2019-01-01

  The present invention relates to a method of biological labeling that occurs via a free radical chain reaction. The labeling occurs due to deposition of a detectable reporter molecule from a media comprising a substance comprising at least two moieties of a peroxidase enzyme substrate (termed herein ‘cross-linker’) in a target site comprising peroxidase activity and a biological marker. The labeling reaction described herein may generally be used to detect targets in a host of experimental schemes for detecting and visualizing a biological or chemical target, including immunohistochemistry (IHC), in situ hybridization (ISH), antibody-based staining methods such as ELISA, Southern, Northern, and Western blotting, and others.

  本发明涉及一种通过自由基链反应进行生物标记的方法。标记发生的原因是可检测的报告分子从一种介质中沉积出来，该介质包含一种物质，该物质至少含有两个过氧化物酶酶底物（此处称为 "交联剂"），该物质位于包含过氧化物酶活性和生物标记物的靶点。本文所述的标记反应一般可用于检测生物或化学靶标的一系列实验方案中的靶标，包括免疫组织化学（IHC）、原位杂交（ISH）、基于抗体的染色方法，如 ELISA、Southern、Northern 和 Western 印迹等。
• Quantification of single target molecules in histological samples
  申请人：Lohse Jesper

  公开号：US10180426B2

  公开（公告）日：2019-01-15

  The present invention lies in the field of visualization and quantification of immobilized targets in samples using immunochemical means. The methods of the invention utilize an immunostaining system allowing visualizing single target units in samples as distinct dots. In particular, the invention relates to methods and reagents for visualization and quantification of molecular targets immunostained in histological samples and use of said method and reagents in medical diagnostic. However, the visualization and quantification methods of the invention are applicable to a variety of targets in different samples and allow precise quantifying both relative and absolute amounts thereof.

  本发明属于利用免疫化学方法对样品中的固定靶标进行可视化和量化的领域。本发明的方法利用一种免疫染色系统，可将样品中的单个目标单位可视化为不同的点。特别是，本发明涉及对组织学样本中免疫染色的分子靶标进行可视化和定量的方法和试剂，以及上述方法和试剂在医学诊断中的应用。不过，本发明的可视化和定量方法适用于不同样本中的各种靶标，并可精确定量其相对量和绝对量。
• Method for evaluation of target in histological sample
  申请人：DAKO DENMARK A/S

  公开号：US10648970B2

  公开（公告）日：2020-05-12

  The present invention lies in the field of visualization and quantification of immobilized targets in samples using immunochemical means. In particular, the invention relates to a method and reagents for detection, visualization and quantification of a molecular target in immunostained histological samples using particular compositions of the target specific binding agent. Methods and compositions of the invention are suitable for any assay that uses a target visualization system in histological samples based on detection of the target by the target specific binding agent. The methods and compositions are useful for evaluation of targets that are biomarkers of diseases in medical diagnostic.

  本发明属于利用免疫化学手段对样品中固定靶标进行可视化和定量的领域。特别是，本发明涉及一种方法和试剂，利用目标特异性结合剂的特定组合物对免疫染色组织学样本中的分子目标进行检测、可视化和定量。本发明的方法和组合物适用于在组织学样本中使用基于靶标特异性结合剂检测靶标的靶标可视化系统的任何检测。本发明的方法和组合物适用于评估医学诊断中作为疾病生物标志物的靶标。
• Combined histological stain
  申请人：Lohse Jesper

  公开号：US10718777B2

  公开（公告）日：2020-07-21

  The present invention relates to methods of visualizing targets in histological samples, e.g. biopsy samples, wherein the methods comprise staining of the sample with (i) one or more target specific immunochemical stains, and (ii) a histological stain for specific tissue components e.g. iron, mucins glycogen, amyloid, nucleic acids, etc., e.g. hematoxilyn and/or eosin stains or the like, that is used to enhance contrast in the microscopic image of a tissue sample, highlight morphologic structures in the sample for viewing, define and examine tissues, cell populations, or organelles within individual cells. Methods may further comprise evaluation of expression of one or more targets in the sample. The disclosed methods are useful for medical diagnostics.

  本发明涉及在组织学样本（如活检样本）中观察目标的方法，其中该方法包括用(i) 一种或多种目标特异性免疫化学染色剂对样本进行染色，以及(ii) 一种特定组织成分的组织学染色剂，如铁、粘蛋白糖原、淀粉样蛋白、核酸等、例如，苏木精和/或伊红染色法或类似方法，用于增强组织样本显微图像的对比度，突出样本中的形态结构以供观察，定义和检查组织、细胞群或单个细胞内的细胞器。方法还可包括评估样本中一个或多个靶点的表达。所公开的方法可用于医学诊断。