3-O-acetyl-6-O-allyl-4-deoxy-4-(thymin-1-yl)-2,5-anhydro-L-mannitol | 936831-17-3

• 分子结构分类: 有机化合物 - 核苷、核苷酸和类似物 - 嘧啶核苷
• 英文名称: 3-O-acetyl-6-O-allyl-4-deoxy-4-(thymin-1-yl)-2,5-anhydro-L-mannitol
• CAS: 936831-17-3
• 化学式: C₁₆H₂₂N₂O₇
• 分子量: 354.36
• InChiKey: XLURNARSLAKARC-XUXIUFHCSA-N

反应信息

• 作为反应物：
  描述：

  3-O-acetyl-6-O-allyl-4-deoxy-4-(thymin-1-yl)-2,5-anhydro-L-mannitol 生成 6-O-allyl-4-deoxy-4-(thymin-1-yl)-2,5-anhydro-L-mannitol-1-triphosphate
  参考文献：
  名称：

  Synthesis and recognition of novel isonucleoside triphosphates by DNA polymerases

  摘要：

  Isonucleosides have been attracting a lot of attention in recent years due to the chemical and enzymatic stability and potential anticancer and antiviral activities. We have reported some of the isonucleosides which exhibited significant anticancer activity and found that the oligonucleotide incorporated with isonucleoside could increase the enzymatic stability against the degradation by phosphodiesterase. In this paper, we investigated the recognition of the isonucleoside triphosphates 1-6 by Taq, Vent(exo(-)), DeepVent(exo(-)), 9 degrees Nm, and Therminator DNA polymerases by a non-radioactivity method. We found that most of the isonucleoside triphosphates can be recognized by various DNA polymerase and act as terminators. Isonucleoside triphosphates 2 and 6 can be incorporated as substrates into the primer at 3' terminus to lengthen the chain dependent on a DNA template by Vent(exo(-)) and DeepVent(exo(-)) DNA polymerases. (c) 2007 Elsevier Ltd. All rights reserved.

  DOI：

  10.1016/j.bmc.2007.02.003
• 作为产物：
  描述：

  3-O-acetyl-6-O-allyl-1-O-(4,4'-dimethoxy)trityl-4-deoxy-4-(thymin-1-yl)-2,5-anhydro-L-mannitol 在 碘 作用下， 以 甲醇 为溶剂， 反应 6.0h， 以80%的产率得到3-O-acetyl-6-O-allyl-4-deoxy-4-(thymin-1-yl)-2,5-anhydro-L-mannitol
  参考文献：
  名称：

  Synthesis and recognition of novel isonucleoside triphosphates by DNA polymerases

  摘要：

  Isonucleosides have been attracting a lot of attention in recent years due to the chemical and enzymatic stability and potential anticancer and antiviral activities. We have reported some of the isonucleosides which exhibited significant anticancer activity and found that the oligonucleotide incorporated with isonucleoside could increase the enzymatic stability against the degradation by phosphodiesterase. In this paper, we investigated the recognition of the isonucleoside triphosphates 1-6 by Taq, Vent(exo(-)), DeepVent(exo(-)), 9 degrees Nm, and Therminator DNA polymerases by a non-radioactivity method. We found that most of the isonucleoside triphosphates can be recognized by various DNA polymerase and act as terminators. Isonucleoside triphosphates 2 and 6 can be incorporated as substrates into the primer at 3' terminus to lengthen the chain dependent on a DNA template by Vent(exo(-)) and DeepVent(exo(-)) DNA polymerases. (c) 2007 Elsevier Ltd. All rights reserved.

  DOI：

  10.1016/j.bmc.2007.02.003