N'-(2-hydroxynaphthalenemethylene)-4-(2-hydroxynaphthalenemethylenamine)benzoylhydrazine | 1246737-73-4

• 分子结构分类: 有机化合物 - 苯类化合物 - 萘
• 英文名称: N'-(2-hydroxynaphthalenemethylene)-4-(2-hydroxynaphthalenemethylenamine)benzoylhydrazine
• 英文别名: N'-(2-hydroxynaphthalenemethylene)-4-(2-hydroxynaphthalenemethylenamino)benzoylhydrazine
• CAS: 1246737-73-4
• 化学式: C₂₉H₂₁N₃O₃
• 分子量: 459.504
• InChiKey: FVLAKMXKCLWIDB-UHFFFAOYSA-N

计算性质

• 辛醇/水分配系数(LogP)：
  None
• 重原子数：
  None
• 可旋转键数：
  None
• 环数：
  None
• sp3杂化的碳原子比例：
  None
• 拓扑面积：
  None
• 氢给体数：
  None
• 氢受体数：
  None

反应信息

• 作为反应物：
  描述：

  zinc(II) perchlorate 、 N'-(2-hydroxynaphthalenemethylene)-4-(2-hydroxynaphthalenemethylenamine)benzoylhydrazine 以 二甲基亚砜 为溶剂， 生成 Zn(N'-(2-hydroxynaphthalenemethylene)-4-(2-hydroxynaphthalenemethylenamine)benzoylhydrazine(1-))
  参考文献：
  名称：

  Study of the interaction between a new Schiff-base complex and bovine serum albumin by fluorescence spectroscopy

  摘要：

  A new Schiff-base compound, N'-(2-hydroxynaphthalenemethylene)-4-(2-hydroxyl naphthalenemethylenamine)benzoylhydrazine (1), was synthesized and the interaction between zinc complex (1-Zn) and bovine serum albumin (BSA) was investigated by fluorescence and absorption spectroscopies. A marked increase in the fluorescence intensity of 1-Zn was observed at 475 nm upon addition of BSA when excitation wavelength was set at 370 nm in pH 7.4 Tris-HCl buffer solution. Reversely, the intrinsic fluorescence of BSA could be quenched by 1-Zn complex. The quenching mechanism was suggested as static quenching according to the Stern-Volmer equation and the UV-vis absorption spectral change of 1-Zn upon addition of BSA. The binding constants K-b and the number of binding sites n were calculated. The effect of 1-Zn on the conformation of BSA was studied using synchronous fluorescence spectroscopy and three-dimensional fluorescence spectroscopy. In addition, the binding average distance r between the donor (BSA) and acceptor (1-Zn) was estimated based on the Forster's non-radiation energy transfer theory. (C) 2010 Elsevier B.V. All rights reserved.

  DOI：

  10.1016/j.saa.2010.06.010
• 作为产物：
  描述：

  4-氨基苯甲酰肼 、 2-羟基-1-萘甲醛 以 乙醇 为溶剂， 反应 1.0h， 生成 N'-(2-hydroxynaphthalenemethylene)-4-(2-hydroxynaphthalenemethylenamine)benzoylhydrazine
  参考文献：
  名称：

  Study of the interaction between a new Schiff-base complex and bovine serum albumin by fluorescence spectroscopy

  摘要：

  A new Schiff-base compound, N'-(2-hydroxynaphthalenemethylene)-4-(2-hydroxyl naphthalenemethylenamine)benzoylhydrazine (1), was synthesized and the interaction between zinc complex (1-Zn) and bovine serum albumin (BSA) was investigated by fluorescence and absorption spectroscopies. A marked increase in the fluorescence intensity of 1-Zn was observed at 475 nm upon addition of BSA when excitation wavelength was set at 370 nm in pH 7.4 Tris-HCl buffer solution. Reversely, the intrinsic fluorescence of BSA could be quenched by 1-Zn complex. The quenching mechanism was suggested as static quenching according to the Stern-Volmer equation and the UV-vis absorption spectral change of 1-Zn upon addition of BSA. The binding constants K-b and the number of binding sites n were calculated. The effect of 1-Zn on the conformation of BSA was studied using synchronous fluorescence spectroscopy and three-dimensional fluorescence spectroscopy. In addition, the binding average distance r between the donor (BSA) and acceptor (1-Zn) was estimated based on the Forster's non-radiation energy transfer theory. (C) 2010 Elsevier B.V. All rights reserved.

  DOI：

  10.1016/j.saa.2010.06.010

文献信息

• Synthesis of the Rheb and K-Ras4B GTPases
  作者：Yong-Xiang Chen、Sebastian Koch、Katharina Uhlenbrock、Katrin Weise、Debapratim Das、Lothar Gremer、Luc Brunsveld、Alfred Wittinghofer、Roland Winter、Gemma Triola、Herbert Waldmann

  DOI：10.1002/anie.201001884

  日期：2010.8.16

  Now available! Farnesylated and carboxymethylated Rheb (see picture) and K‐Ras4B GTPases were synthesized in useful amounts by a combination of expressed protein ligation and solid‐phase lipopeptide synthesis. The functionality of the proteins was proven by biochemical, biophysical, and cell‐based investigations.

  现在可用！法尼基化和羧甲基化的Rheb（见图）和K‐Ras4B GTPases通过表达的蛋白质连接和固相脂肽合成相结合，以有用的量合成。蛋白质的功能已通过生化，生物物理和基于细胞的研究证明。