The present invention provides a method for determining whether or not a test sample contains a phytopathogenic fungus. The method comprises (a) putting the test sample on a front surface of a substrate comprising a through hole; wherein the substrate comprises a cellulose film on the back surface thereof; the through hole of the substrate has a cross-sectional area of not less than 7.065 square micrometers and not more than 19.625 square micrometers; the cellulose film has no through hole; and the cellulose film has a thickness of more than 0.5 micrometers and not more than 3.7 micrometers; (b) leaving the test sample at rest after the step (a); (c) irradiating the cellulose film with ultraviolet light using an ultraviolet light source provided so as to face the front surface of the cellulose film after the step (b); (d) bringing a back surface of the cellulose film into contact with a fungus color reaction reagent after the step (c); and (e) determining that the test sample contains the phytopathogenic fungus, if a color is given to the fungus color reaction reagent.
本发明提供了一种确定测试样品是否含有植物病原真菌的方法。该方法包括:(a) 将测试样品放在具有通孔的基底的正面;其中基底的背面包括
纤维素薄膜;基底的通孔的横截面积不小于 7.065 平方微米,不大于 19.625 平方微米;
纤维素薄膜没有通孔;
纤维素薄膜的厚度大于 0.5 微米,不大于 3.7 微米;(b) 在步骤(a)之后使测试样品静止;(c) 在步骤(b)之后,使用紫外线光源用紫外线照射
纤维素膜,紫外线光源的设置应面向
纤维素膜的正面;(d) 在步骤(c)之后,使
纤维素膜的背面与真菌颜色反应试剂接触;(e) 如果真菌颜色反应试剂呈现颜色,则确定测试样品含有植物病原真菌。