Disclosed is a method of attaching, indirectly, a member of a specific binding pair (or sbp) to a surface, the method comprising the steps of: (a) contacting the surface with a solution, preferably an aqueous solution, of a polymer, having side chains according to the formula X-Y-Z-R, wherein X is a spacer group; Y is a sulphur, selenium or tellurium atom; Z is a sulphur, selenium or tellurium atom, any of which may be bonded to one or two oxygen atoms; and wherein R is any suitable moiety such that -Z-R constitutes a leaving group; such that at least some of the -Z-R groups are displaced and the polymer becomes bound to the surface by X-Y groups; and (b) contacting a polymer-coated surface resulting from step (a) with a solution, preferably an aqueous solution, comprising an sbp member, so as to cause the polymer to react with the sbp member, so as to attach the sbp member, indirectly, to the surface.
公开了一种间接地将特定结合对(或sbp)的成员附着到表面的方法,该方法包括以下步骤:(a)将表面与具有侧链的聚合物的溶液接触,该侧链符合以下公式X-Y-Z-R,其中X是一个空间群;Y是
硫、
硒或
碲原子;Z是
硫、
硒或
碲原子,其中任何一个可能与一个或两个氧原子结合;其中R是任何适当的基团,使得-Z-R构成一个脱离基团;使得至少一些-Z-R基团被取代,聚合物通过X-Y基团结合到表面;和(b)将步骤(a)得到的涂有聚合物的表面与包含sbp成员的溶液接触,从而使聚合物与sbp成员发生反应,将sbp成员间接地附着到表面。