o-Nitrotyramine | 3998-61-6

• 分子结构分类: 有机化合物 - 苯类化合物 - 酚类
• 英文名称: o-Nitrotyramine
• 英文别名: 4-(2-aminoethyl)-3-nitrophenol
• CAS: 3998-61-6
• 化学式: C8H10N2O3
• 分子量: 182.18
• InChiKey: MFILUFKQTVSEFX-UHFFFAOYSA-N

计算性质

• 辛醇/水分配系数(LogP)：
  0.7
• 重原子数：
  13
• 可旋转键数：
  2
• 环数：
  1.0
• sp3杂化的碳原子比例：
  0.25
• 拓扑面积：
  92.1
• 氢给体数：
  2
• 氢受体数：
  4

反应信息

• 作为产物：
  描述：

  对羟基苯乙胺 、 硝酸 、 三氟乙酸 生成 o-Nitrotyramine
  参考文献：
  名称：

  MODIFIED FC POLYPEPTIDES, FC CONJUGATES, AND METHODS OF USE THEREOF

  摘要：

  本公开提供了经过修饰的Fc区域，其中具有一个或多个附着基团，用于附着异源功能基团；以及生成经过修饰的Fc区域的方法。本公开提供了Fc共轭物；以及制备共轭物的方法。Fc共轭物在各种方法中有用，这些方法也提供了。

  公开号：

  US20150218258A1

文献信息

• [EN] AFFINITY-ASSISTED PROTEIN MODIFICATION AND RECYCLING<br/>[FR] MODIFICATION ET RECYCLAGE DE PROTÉINES BASÉS SUR LEUR AFFINITÉ
  申请人：UNIV CALIFORNIA

  公开号：WO2016118770A1

  公开（公告）日：2016-07-28

  The present invention provides methods for preparing a protein conjugate having a defined number of conjugate groups. The method includes: forming a mixture containing a macrocyclic matrix material and a plurality of proteins; eluting the proteins from the macrocyclic matrix material to obtain a first separated protein fraction and a second separated protein fraction, wherein substantially all of the proteins in the first separated protein fraction have the same number of handle moieties; contacting the handle moieties with a conversion reagent under conditions sufficient to convert the handle moieties in the first separated protein fraction to reactive moieties; and contacting the reactive moieties with a conjugation reagent under conditions sufficient to form a plurality of protein conjugates, wherein substantially all of the protein conjugates in the plurality have the same number of conjugate groups. Also disclosed are methods for recovering enzymes and other proteins from mixtures for isolation and/or reuse of the enzymes and proteins.

  本发明提供了制备具有定义数量结合基团的蛋白共轭物的方法。该方法包括：形成含有大环矩阵材料和多种蛋白质的混合物；从大环矩阵材料中洗脱蛋白质，以获得第一分离的蛋白质分数和第二分离的蛋白质分数，其中第一分离的蛋白质分数中几乎所有蛋白质具有相同数量的手柄基团；将手柄基团与转化试剂接触，在足以将第一分离的蛋白质分数中的手柄基团转化为反应性基团的条件下；将反应性基团与结合试剂接触，在足以形成多种蛋白共轭物的条件下，其中多数蛋白共轭物具有相同数量的结合基团。还公开了从混合物中回收酶和其他蛋白质以便分离和/或重复使用酶和蛋白质的方法。
• Affinity-assisted protein modification and recycling
  申请人：The Regents Of The University Of California

  公开号：US10370407B2

  公开（公告）日：2019-08-06

  Methods for preparing a protein conjugate having a defined number of conjugate groups are provided. The method includes: forming a mixture containing a macrocyclic matrix material and a plurality of proteins; eluting the proteins to obtain a first separated protein fraction and a second separated protein fraction, wherein substantially all of the proteins in the first separated protein fraction have the same number of handle moieties; contacting the handle moieties with a conversion reagent under conditions sufficient to convert the handle moieties in the first separated protein fraction to reactive moieties; and contacting the reactive moieties with a conjugation reagent under conditions sufficient to form a plurality of protein conjugates, wherein substantially all of the protein conjugates in the plurality have the same number of conjugate groups. Methods also include recovering enzymes and other proteins from mixtures for isolation and/or reuse of the enzymes and proteins.

  本研究提供了制备具有一定数量共轭基团的蛋白质共轭物的方法。该方法包括形成含有大环基质材料和多种蛋白质的混合物；洗脱蛋白质以获得第一分离蛋白质馏分和第二分离蛋白质馏分，其中第一分离蛋白质馏分中的蛋白质基本上都具有相同数量的柄分子；在足以将第一分离蛋白馏分中的手柄分子转化为活性分子的条件下，将手柄分子与转化试剂接触；以及 在足以形成多个蛋白共轭物的条件下，将活性分子与共轭试剂接触，其中多个蛋白共轭物中的所有蛋白共轭物都具有相同数量的共轭基团。方法还包括从混合物中回收酶和其他蛋白质，以分离和/或重复使用酶和蛋白质。
• AFFINITY-ASSISTED PROTEIN MODIFICATION AND RECYCLING
  申请人：The Regents Of The University Of California

  公开号：US20180148472A1

  公开（公告）日：2018-05-31

  Methods for preparing a protein conjugate having a defined number of conjugate groups are provided. The method includes: forming a mixture containing a macrocyclic matrix material and a plurality of proteins; eluting the proteins to obtain a first separated protein fraction and a second separated protein fraction, wherein substantially all of the proteins in the first separated protein fraction have the same number of handle moieties; contacting the handle moieties with a conversion reagent under conditions sufficient to convert the handle moieties in the first separated protein fraction to reactive moieties; and contacting the reactive moieties with a conjugation reagent under conditions sufficient to form a plurality of protein conjugates, wherein substantially all of the protein conjugates in the plurality have the same number of conjugate groups. Methods also include recovering enzymes and other proteins from mixtures for isolation and/or reuse of the enzymes and proteins.
• US9834597B2
  申请人：——

  公开号：US9834597B2

  公开（公告）日：2017-12-05
• MODIFIED FC POLYPEPTIDES, FC CONJUGATES, AND METHODS OF USE THEREOF
  申请人：The Regents of the University of California

  公开号：US20150218258A1

  公开（公告）日：2015-08-06

  The present disclosure provides modified Fc domains having one or more attachment moieties for attaching a heterologous functional moiety; and methods of generating the modified Fc domains. The present disclosure provides Fc conjugates; and methods of making the conjugates. The Fc conjugates are useful in various methods, which are also provided.

  本公开提供了经过修饰的Fc区域，其中具有一个或多个附着基团，用于附着异源功能基团；以及生成经过修饰的Fc区域的方法。本公开提供了Fc共轭物；以及制备共轭物的方法。Fc共轭物在各种方法中有用，这些方法也提供了。