3-(10H-phenothiazin-10-ium-10-yl)propane-1-sulfonate | 129808-92-0

• 分子结构分类: 有机化合物 - 有机杂环化合物 - 苯并噻嗪类
• 英文名称: 3-(10H-phenothiazin-10-ium-10-yl)propane-1-sulfonate
• CAS: 129808-92-0
• 化学式: C15H15NO3S2
• 分子量: 321.4
• InChiKey: ONVGBCNKVCPJCI-UHFFFAOYSA-N

计算性质

• 辛醇/水分配系数(LogP)：
  3.4
• 重原子数：
  21
• 可旋转键数：
  4
• 环数：
  3.0
• sp3杂化的碳原子比例：
  0.2
• 拓扑面积：
  91.3
• 氢给体数：
  1
• 氢受体数：
  5

文献信息

• Compounds for generating chemiluminescence with a peroxidase
  申请人：——

  公开号：US20030170762A1

  公开（公告）日：2003-09-11

  Novel compounds comprising a C—C double bond substituted at one carbon with two sulfur atom-containing groups are disclosed. The compounds are useful in methods and compositions for generating chemiluminescence rapidly by reaction with a peroxidase enzyme and a peroxide. The chemiluminescence thus produced can be used as a detectable signal in assays for peroxidase enzymes or peroxide-producing enzymes and in assays employing enzyme-labeled specific binding pairs.

  披露了包含C-C双键的全新化合物，其中一个碳原子上带有两个含硫原子的基团。这些化合物在通过与过氧化物酶酶和过氧化物反应来快速产生化学发光的方法和组合物中有用。由此产生的化学发光可用作检测过氧化物酶或产过氧化物酶的酶的检测信号，以及在利用酶标记的特定结合对进行的分析中。
• Preparation of high purity phenothiazine N-alkylsulfonates and their use in chemiluminescent assays for the measurement of peroxidase acitivity
  申请人：Della Ciana Leopoldo

  公开号：US20080176251A1

  公开（公告）日：2008-07-24

  A process is described for preparing, efficiently and with a high degree of purity N-alkylsulfonates of phenothiazine. The process consists in (a) the preparation of the phenothiazine anion, and (b) the reaction of said anion with cyclic alkyl sulfonates, such as 1,3-propane sultone and 1,4-butane sultone. This process is simpler, more direct, and more efficient than the procedures currently used for the synthesis of N-alkylsulfonates derivatives of phenothiazine. In addition, the products obtained with this process are far more pure than those obtained through current procedures and therefore ideal for bioanalytical applications that require high sensitivity, such as assays based on the measurement of peroxidase activity using chemiluminescence.

  描述了一种制备苯并噻嗪N-烷基磺酸盐的过程，高效且纯度较高。该过程包括(a)制备苯并噻嗪负离子，以及(b)将该负离子与环状烷基磺酸盐（如1,3-丙烷磺酸内酯和1,4-丁烷磺酸内酯）反应。这一过程比目前用于合成苯并噻嗪N-烷基磺酸盐衍生物的程序更简单、更直接、更高效。此外，用这一过程得到的产品比通过当前程序得到的产品更纯，因此非常适合需要高灵敏度的生物分析应用，如基于化学发光法测定过氧化物酶活性的分析。
• Method for the chemiluminescence assay of the activity of peroxidase
  申请人：FUJIREBIO INC.

  公开号：EP0361470A2

  公开（公告）日：1990-04-04

  A method of chemiluminescence assay of the activity of peroxidase is disclosed, which employes (i) luminol or isoluminol as a substrate, (ii) hydrogen peroxide, and (iii) at lease one compound, serving as an enhancer for the activity of peroxidase, selected from the group consisting of wherein R¹ represents a hydroxyl group, an alkali metal salt of hydroxyl group (-OM, where M is an alkali metal), an amino group which may have a substituent, a halogen, an alkyl group having 1 to 4 carbon atoms, which may have a substituent; R² and R³ each represent hydrogen or a halogen; R⁴ and R⁵ each represent hydrogen, an alkyl group having 1 to 4 carbon atoms, which may have a substituent, or an alkoxyl group having 1 to 4 carbon atoms, which may have a substituent; wherein R⁶ represents an alkyl group having 1 to 6 carbon atoms, which may have a substituent, or an alkene group having 2 to 6 carbon atoms, which may have a substituent, R⁷ to R¹⁰ each represent hydrogen, an alkyl group having 1 to 6 carbon atoms, which may have a substituent, or a halogen, X represents oxygen or sulfur, Y represents -CH- or nitrogen, which may be the same or different, and Y′ represents carbon or nitrogen, which may be the same or different.

  本发明公开了一种过氧化物酶活性的化学发光检测方法，该方法采用(i)发光酚或异鲁米诺作为底物，(ii)过氧化氢，和(iii)至少一种化合物作为过氧化物酶活性的增强剂、选自由下列物质组成的组 其中 R¹ 代表羟基、羟基的碱金属盐（-OM，其中 M 为碱金属）、可能具有取代基的氨基、卤素、具有 1 至 4 个碳原子且可能具有取代基的烷基；R² 和 R³ 分别代表氢或卤素；R⁴ 和 R⁵ 分别代表氢、1-4 个碳原子的烷基（可带有取代基）或 1-4 个碳原子的烷氧基（可带有取代基）； 其中 R⁶ 代表具有 1 至 6 个碳原子的烷基，该烷基可能具有取代基，或具有 2 至 6 个碳原子的烯基，该烯基可能具有取代基，R⁷ 至 R¹⁰ 各自代表氢、具有 1 至 6 个碳原子的烷基、X代表氧或硫，Y代表-CH-或氮，它们可以相同或不同，Y′代表碳或氮，它们可以相同或不同。
• Immunochemical assay method with plural items
  申请人：FUJIREBIO INC.

  公开号：EP0440193A2

  公开（公告）日：1991-08-07

  There is disclosed an immunochemical assay method capable of simultaneously measuring plural items which comprises contacting mixed particles each having an average diameter of 0.1 to 1.0 µm and containing at least two kinds of particles, to which different kind of an antigen or antibody to be assayed is separately bound, with a specimen in a single reaction phase.

  本发明公开了一种能够同时测量多个项目的免疫化学检测方法，该方法包括将平均直径为 0.1 至 1.0 微米的混合微粒（每个微粒都含有至少两种微粒，不同种类的抗原或抗体分别与这些微粒结合）与试样在单一反应相中接触。
• Chemiluminescent analytical method
  申请人：Hitachi Chemical Co., Ltd.

  公开号：EP0650044A2

  公开（公告）日：1995-04-26

  A chemiluminescent method is provided for the analysis of a target. A peroxidase is first bound as a labelling substance to the target by using a target specific binding reagent. The target so labelled is isolated and then reacted with luminol, hydrogen peroxide and an enhancer in an aqueous solvent. The luminescence of light is detected and measured. The aqueous solvent contains at least one protein selected from the group consisting of skim milk and egg albumin. Preferably, the aqueous solvent also contains a non-ionic surfactant such as a polyoxyethylene ether and/or a sugar alcohol such as mannitol.

  为分析目标物提供了一种化学发光方法。首先使用目标物特异性结合试剂将过氧化物酶作为标记物质与目标物结合。这样标记的目标物被分离出来，然后在水性溶剂中与发光酚、过氧化氢和增强剂反应。对发光进行检测和测量。水溶剂中至少含有一种蛋白质，该蛋白质选自脱脂牛奶和鸡蛋白蛋白。水性溶剂最好还含有非离子表面活性剂，如聚氧乙烯醚和/或糖醇，如甘露醇。