17α,20;20,21-bismethylenedioxy-[1,1,19,19,19-2H5]pregn-4-ene-6β-ol-11-one | 638163-32-3

• 分子结构分类: 有机化合物 - 脂质和类脂质分子 - 类固醇和类固醇衍生物
• 英文名称: 17α,20;20,21-bismethylenedioxy-[1,1,19,19,19-2H5]pregn-4-ene-6β-ol-11-one
• 英文别名: 6β-hydroxycortisone-2H5-BMD
• CAS: 638163-32-3
• 化学式: C₂₃H₃₀O₇
• 分子量: 423.447
• InChiKey: AZZUBOGSKOUMOH-WXXSPEEDSA-N

计算性质

• 辛醇/水分配系数(LogP)：
  2.11
• 重原子数：
  30.0
• 可旋转键数：
  1.0
• 环数：
  6.0
• sp3杂化的碳原子比例：
  0.83
• 拓扑面积：
  91.29
• 氢给体数：
  1.0
• 氢受体数：
  7.0

反应信息

• 作为反应物：
  描述：

  17α,20;20,21-bismethylenedioxy-[1,1,19,19,19-2H5]pregn-4-ene-6β-ol-11-one 在 氢氟酸 作用下， 以 四氢呋喃 、 乙醇 为溶剂， 以34.8%的产率得到6β,17α,21-trihydroxy-[1,1,19,19,19-2H5]pregn-4-ene-3,11,20-trione
  参考文献：
  名称：

  Syntheses of stable isotope-labeled 6β-hydroxycortisol, 6β-hydroxycortisone, and 6β-hydroxytestosterone

  摘要：

  A method is described for the preparation of two types of multi-labeled 6beta-hydroxycortisol containing either five deuterium atoms at C-19 methyl and C-1 methylene or four C-13 atoms at C-1, C-2, C-4, and C-19 in addition to the five deuterium atoms for use as analytical internal standards for gas chromatography-mass spectrometry (GC-MS). BMD derivatives of [1,1,19,19,19-H-2(5)]cortisone and [1,2,4,19-C-13(4),1,1,19,19,19-H-2(5)]cortisone (cortisone-H-2(5)-BMD and cortisone-C-13(4), H-2(5)-BMD) were first synthesized via indan synthon method starting from optical active 11-oxoindanylpropionic acid and labeled isopropenyl anion([1,1,3,3,3 1,3,3,3-H-2(5)]- or [1,3-C-13(2),1,1,3,3,3-H-2(5)] isopropenyl anion). The labeled isopropenyl anion was prepared from commercially available [1,1,1,3,3,3-H-2(6)]- or [1,3-C-13(2),1,1,1,3,3,3-H-2(6)]acetone. Ultraviolet (UV) irradiated autoxidation at C-6 position of 3-ethyl-3,5-dienol ether derivatives of the labeled cortisone-BMDs gave 6beta-hydroxy-[1,1,19,19,19-H-2(5)]cortisone-BMD and 6beta-hydroxy-[1,2,4,19-C-13(4),1,1,19,19,19-H-2(5)]cortisone-BMD, respectively, as a mixture of 6beta- and 6alpha-epimers in a ratio of 4:1. Separation of 6beta- and 6alpha-epimers by thin-layer chromatography (TLC) and subsequent hydrolysis of the BMD group at C-17 gave pure labeled 6beta-hydroxycortisone. After protecting the keto group at C-3 of the labeled 6beta-hydroxycortisone-BMD as semicarbazone, reduction of 11-keto group with NaBH4 and subsequent removal of the C-3 and C-17 protecting groups gave 6beta-hydroxy-[1,1,19,19,19-H-2(5)]cortisol (6beta-hydroxycortisol-H-2(5)) and 6beta-hydroxy-[1,2,4,19-C-13(4),1,1,19,19,19-H-2(5)] cortisol (6beta-hydroxycortisol-C-13(4), H-2(5)), respectively, as a mixture of 6beta- and 6alpha-epimers (6beta:6alpha = 4.4:1). The isotopic compositions of 6beta-hydroxycortisol-H-2(5) and 6beta-hydroxycortisol-C-13(4), H-2(5) were 90.9 and 92.1 at.%, respectively. Furthermore, 6beta-hydroxy-[1alpha,16,16,17alpha-H-2(4)]testosterone was synthesized by the UV irradiated autoxidation at C-6 position of 3-ethyl-3,5-dienol ether derivative of deuterium-labeled testosterone ([1alpha,16,16,17alpha-H-2(4)]testosterone) obtained by using catalytic deuteration and hydrogen-deuterium exchange reactions. (C) 2003 Elsevier Inc. All rights reserved.

  DOI：

  10.1016/s0039-128x(03)00102-8
• 作为产物：
  描述：

  <1,1,19,19,19-(2)H>-17α,20;20,21-bismethylenedioxypregn-4-ene-3,11-dione 在 硫酸 作用下， 以 1,4-二氧六环 、 乙醇 为溶剂， 反应 4.17h， 生成 17α,20;20,21-bismethylenedioxy-[1,1,19,19,19-2H5]pregn-4-ene-6β-ol-11-one
  参考文献：
  名称：

  Syntheses of stable isotope-labeled 6β-hydroxycortisol, 6β-hydroxycortisone, and 6β-hydroxytestosterone

  摘要：

  A method is described for the preparation of two types of multi-labeled 6beta-hydroxycortisol containing either five deuterium atoms at C-19 methyl and C-1 methylene or four C-13 atoms at C-1, C-2, C-4, and C-19 in addition to the five deuterium atoms for use as analytical internal standards for gas chromatography-mass spectrometry (GC-MS). BMD derivatives of [1,1,19,19,19-H-2(5)]cortisone and [1,2,4,19-C-13(4),1,1,19,19,19-H-2(5)]cortisone (cortisone-H-2(5)-BMD and cortisone-C-13(4), H-2(5)-BMD) were first synthesized via indan synthon method starting from optical active 11-oxoindanylpropionic acid and labeled isopropenyl anion([1,1,3,3,3 1,3,3,3-H-2(5)]- or [1,3-C-13(2),1,1,3,3,3-H-2(5)] isopropenyl anion). The labeled isopropenyl anion was prepared from commercially available [1,1,1,3,3,3-H-2(6)]- or [1,3-C-13(2),1,1,1,3,3,3-H-2(6)]acetone. Ultraviolet (UV) irradiated autoxidation at C-6 position of 3-ethyl-3,5-dienol ether derivatives of the labeled cortisone-BMDs gave 6beta-hydroxy-[1,1,19,19,19-H-2(5)]cortisone-BMD and 6beta-hydroxy-[1,2,4,19-C-13(4),1,1,19,19,19-H-2(5)]cortisone-BMD, respectively, as a mixture of 6beta- and 6alpha-epimers in a ratio of 4:1. Separation of 6beta- and 6alpha-epimers by thin-layer chromatography (TLC) and subsequent hydrolysis of the BMD group at C-17 gave pure labeled 6beta-hydroxycortisone. After protecting the keto group at C-3 of the labeled 6beta-hydroxycortisone-BMD as semicarbazone, reduction of 11-keto group with NaBH4 and subsequent removal of the C-3 and C-17 protecting groups gave 6beta-hydroxy-[1,1,19,19,19-H-2(5)]cortisol (6beta-hydroxycortisol-H-2(5)) and 6beta-hydroxy-[1,2,4,19-C-13(4),1,1,19,19,19-H-2(5)] cortisol (6beta-hydroxycortisol-C-13(4), H-2(5)), respectively, as a mixture of 6beta- and 6alpha-epimers (6beta:6alpha = 4.4:1). The isotopic compositions of 6beta-hydroxycortisol-H-2(5) and 6beta-hydroxycortisol-C-13(4), H-2(5) were 90.9 and 92.1 at.%, respectively. Furthermore, 6beta-hydroxy-[1alpha,16,16,17alpha-H-2(4)]testosterone was synthesized by the UV irradiated autoxidation at C-6 position of 3-ethyl-3,5-dienol ether derivative of deuterium-labeled testosterone ([1alpha,16,16,17alpha-H-2(4)]testosterone) obtained by using catalytic deuteration and hydrogen-deuterium exchange reactions. (C) 2003 Elsevier Inc. All rights reserved.

  DOI：

  10.1016/s0039-128x(03)00102-8