1-[(R)-3-tert-Butyldisulfanyl-2-(9H-fluoren-9-ylmethoxycarbonylamino)-propionyl]-piperidine-4-carboxylic acid 2,5-dioxo-pyrrolidin-1-yl ester | 724783-38-4

• 分子结构分类: 有机化合物 - 苯类化合物 - 芴
• 英文名称: 1-[(R)-3-tert-Butyldisulfanyl-2-(9H-fluoren-9-ylmethoxycarbonylamino)-propionyl]-piperidine-4-carboxylic acid 2,5-dioxo-pyrrolidin-1-yl ester
• CAS: 724783-38-4
• 化学式: C₃₂H₃₇N₃O₇S₂
• 分子量: 639.794
• InChiKey: LIZYMRBFWISJGD-SANMLTNESA-N

计算性质

• 辛醇/水分配系数(LogP)：
  4.92
• 重原子数：
  44.0
• 可旋转键数：
  9.0
• 环数：
  5.0
• sp3杂化的碳原子比例：
  0.47
• 拓扑面积：
  122.32
• 氢给体数：
  1.0
• 氢受体数：
  9.0

反应信息

• 作为产物：
  描述：

  N-α-Fmoc-S-tert-butylsulfenyl-L-cysteine pentafluorophenyl ester 在 O-(1H-benzotriazol-1-yl)-N,N,N',N'-tetramethyluronium hexafluorophosphate 、 N,N-二异丙基乙胺 作用下， 以 N,N-二甲基甲酰胺 为溶剂， 反应 11.0h， 生成 1-[(R)-3-tert-Butyldisulfanyl-2-(9H-fluoren-9-ylmethoxycarbonylamino)-propionyl]-piperidine-4-carboxylic acid 2,5-dioxo-pyrrolidin-1-yl ester
  参考文献：
  名称：

  Site-specific conjugation of oligonucleotides to the C-terminus of recombinant protein by expressed protein ligation

  摘要：

  We developed a convenient method for synthesizing homogeneous DNA-protein conjugates. The method is based on expressed protein ligation of intein-fusion proteins and oligonucleotides derivatized with a cysteine. A range of cysteinyl oligonucleotides were synthesized by using a new reagent I and were successfully applied to expressed protein ligation to attach the oligonucleotides specifically at the C-terminus of a recombinant protein. (C) 2004 Elsevier Ltd. All rights reserved.

  DOI：

  10.1016/j.bmcl.2004.03.023