An improved method of making a series of bead or microsphere or particle populations characterized by subtle variation in a proportion or ratio of at least two fluorescent dyes distributed within a single bead of each population is provided. These beads, when excited by a single excitation light source are capable of giving off several fluorescent signals simultaneously. A set containing as many as 64 distinct populations of multicolored, fluorescent beads is provided and when combined with analytical reagents bound to the surface of such beads is extremely useful for multiplexed analysis of a plurality of analytes in a single sample. Thus, methods of staining polymeric particles, the particles themselves, and methods of using such particles are claimed.
本发明提供了一种改进的方法,用于制造一系列珠子或微球或粒子群,其特征是在每个群的单个珠子中至少分布有两种荧光
染料,其比例或比率有微妙的变化。这些珠子在单个激发光源的激发下能够同时发出多个荧光信号。本发明提供了一套包含多达 64 个不同种群的多色荧光珠,当与结合在这些珠子表面的
分析试剂结合使用时,可对单个样品中的多种分析物进行多重分析。因此,本发明提出了对聚合物微粒进行染色的方法、微粒本身以及使用这种微粒的方法。