DOPE

• 分子结构分类: 有机化合物 - 脂质和类脂质分子 - 甘油磷脂
• 英文名称: DOPE
• 英文别名: 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine；2-Azaniumylethyl 2,3-di(octadec-9-enoyloxy)propyl phosphate
• 化学式: C₄₁H₇₈NO₈P
• 分子量: 744.046
• InChiKey: MWRBNPKJOOWZPW-UHFFFAOYSA-N

计算性质

• 辛醇/水分配系数(LogP)：
  10.6
• 重原子数：
  51
• 可旋转键数：
  41
• 环数：
  0.0
• sp3杂化的碳原子比例：
  0.85
• 拓扑面积：
  134
• 氢给体数：
  2
• 氢受体数：
  9

反应信息

• 作为反应物：
  描述：

  4-(2-((1E,3E,5E,7E)-7-(3-(2-carboxyethyl)-1,1-dimethyl-1H-benzo[e]indol-2(3H)-ylidene)hepta-1,3,5-trienyl)-1,1-dimethyl-1H-benzo[e]indolium-3-yl)butane-1-sulfonic acid 、 DOPE 在 N-羟基丁二酰亚胺 、 N,N'-二环己基碳二亚胺 、 三乙胺 作用下， 以 氯仿 、 乙腈 为溶剂， 反应 3.5h， 以0.37 g的产率得到
  参考文献：
  名称：

  Fluorescent Probe for Imaging Lymph Nodes

  摘要：

  本发明公开了一种近红外荧光成像剂，包括吲哚菁绿类荧光染料和脂质体。本发明的近红外荧光成像剂在哨兵淋巴结中表现出高荧光强度和长时间的锚定，因此适用于在哨兵淋巴结导航手术中检测哨兵淋巴结。本发明还公开了一种吲哚菁绿衍生物，特别适用于本发明的近红外荧光成像剂中使用。

  公开号：

  US20160222211A1

文献信息

• Preparation method of adiposomes, and use thereof
  申请人：Institute Of Biophysics, Chinese Academy Of Sciences

  公开号：US10987431B2

  公开（公告）日：2021-04-27

  A preparation method of adiposomes, and use thereof. Provided is a method for preparing adiposomes consisting of neutral lipids and a monolayer phospholipid membrane, comprising a1) vortexing phospholipid and neutral lipids in a buffer, centrifuging the resulting mixture, and collecting an upper liquid phase; a2) purifying the upper liquid phase twice or more by uniformly mixing the upper liquid phase with the buffer, layering the mixture, and collecting an upper liquid phase; and a3) uniformly mixing the upper liquid phase obtained in step a2) with the buffer, layering the mixture, and collecting a lower liquid phase in containing adiposomes. For the adiposomes prepared by the method, one or more resident proteins and/or functional proteins can be recruited to obtain artificial lipid droplets, and one or more apolipoproteins can be recruited to obtain artificial lipoproteins; and they all play important roles in preparing drugs and/or drug carriers.

  一种脂肪体的制备方法及其用途。本发明提供了一种由中性脂质和单层磷脂膜组成的脂肪体的制备方法，包括 a1) 在缓冲液中涡旋磷脂和中性脂质，离心所得混合物，收集上层液相；a2) 将上层液相与缓冲液均匀混合，将混合物分层，收集上层液相，对上层液相进行两次或两次以上的纯化；以及 a3) 将步骤 a2) 中得到的上层液相与缓冲液均匀混合，将混合物分层，收集下层液相，使其含有脂肪体。对于用该方法制备的脂肪体，可加入一种或多种常驻蛋白和/或功能蛋白以获得人工脂滴，还可加入一种或多种载脂蛋白以获得人工脂蛋白；它们在制备药物和/或药物载体中都发挥着重要作用。
• LC-MS/MS analysis of carboxymethylated and carboxyethylated phosphatidylethanolamines in human erythrocytes and blood plasma
  作者：Naoki Shoji、Kiyotaka Nakagawa、Akira Asai、Ikuko Fujita、Aya Hashiura、Yasushi Nakajima、Shinichi Oikawa、Teruo Miyazawa

  DOI：10.1194/jlr.d004564

  日期：2010.8

  An amino group of phosphatidylethanolamine (PE) is considered as a target for nonenzymatic glycation, and the potential involvement of lipid glycation in the pathogenesis of diabetic complications has generated interest. However, unlike an early glycation product of PE (Amadori-PE), the occurrence and roles of advanced glycation end products of PE (AGE-PE) in vivo have been unclear. Here, we developed an LC-MS/MS method for the analysis of AGE-PE [carboxymethyl-PE (CM-PE) and carboxyethyl- PE (CE-PE)]. Collision-induced dissociation of CM-PE and CE-PE produced characteristic ions, permitting neutral loss scanning (NLS) and multiple reaction monitoring (MRM) of AGE-PE. By NLS analysis, a series of AGE-PE molecular species was detected in human erythrocytes and blood plasma. In LC-MS/MS analysis, MRM enabled the separation and determination of the predominant AGE-PE species. Between healthy subjects and diabetic patients, no significant differences were observed in AGE-PE concentrations in erythrocytes and plasma, whereas Amadori-PE concentrations were higher in diabetic patients. These results provide direct evidence for the presence of AGE-PE in human blood, and indicated that, compared with Amadori-PE, AGE-PE is less likely to be accumulated in diabetic blood. The presently developed LC-MS/MS method appears to be a powerful tool for understanding in vivo lipid glycation and its pathophysiological consequence.-Shoji, N., K. Nakagawa, A. Asai, I. Fujita, A. Hashiura, Y. Nakajima, S. Oikawa, and T. Miyazawa. LC-MS/MS analysis of carboxymethylated and carboxyethylated phosphatidylethanolamines in human erythrocytes and blood plasma. J. Lipid Res. 51: 2445-2453.