4-Azido-2-nitroaniline | 66066-79-3

• 分子结构分类: 有机化合物 - 苯类化合物 - 苯和取代衍生物
• 英文名称: 4-Azido-2-nitroaniline
• CAS: 66066-79-3
• 化学式: C6H5N5O2
• 分子量: 179.14
• InChiKey: PWJBLRAJKVZZKN-UHFFFAOYSA-N

计算性质

• 辛醇/水分配系数(LogP)：
  2.1
• 重原子数：
  13
• 可旋转键数：
  1
• 环数：
  1.0
• sp3杂化的碳原子比例：
  0.0
• 拓扑面积：
  86.2
• 氢给体数：
  1
• 氢受体数：
  5

文献信息

• Diagnostic device incorporating a biochemical ligand
  申请人：NATIONAL RESEARCH DEVELOPMENT CORPORATION

  公开号：EP0127438A1

  公开（公告）日：1984-12-05

  The invention relates to a device useful in diagnostics in which a biochemical species is attached to the surface of a sensor, especiallyto the gate of field effecttransistor. In such a "BIOCHEMFET" it has been a problem to attach two or more biochemical species to the surface of the sensor, especially to the gates of a multi-gated FET. It has now been found possible to bond a group having a photoactivatable function covalently to the sensor surface, photo-expose the resultant modified surface selectively, e.g. through a mask, and bond the biochemical species, e.g. a hapten, antigen, antibody, lectin or enzyme, to the photoactivated function. In this way the biochemical species becomes attached in selected areas only. In view of the success of this technique realised under conditions of miniaturisation, the invention makes possible "printed circuits for proteins". The invention includes the device, process of manufacture thereo, use for diagnostic purposes and a diagnostic kit of the device and a partner capable of binding tothe biochemical species.

  本发明涉及一种用于诊断的装置，在这种装置中，生化物质被附着在传感器的表面，特别是场效应晶体管的栅极上。在这种 "生物化学场效应晶体管 "中，将两种或两种以上的生化物质附着在传感器表面，特别是附着在多门场效应晶体管的栅极上一直是个问题。现在，我们发现可以将具有光活化功能的基团共价键合到传感器表面，有选择性地（例如通过掩膜）对修饰后的表面进行光曝光，然后将生化物质（例如合生元、抗原、抗体、凝集素或酶）键合到光活化功能上。这样，生化物质只附着在选定的区域。鉴于这项技术在微型化条件下取得的成功，本发明使 "蛋白质印刷电路 "成为可能。 本发明包括装置、制造工艺、诊断用途以及由装置和能与生化物质结合的伴侣组成的诊断套件。
• Method for generating aptameters with improved off-rates
  申请人：Somalogic, Inc.

  公开号：EP2172566A1

  公开（公告）日：2010-04-07

  The present disclosure describes improved SELEX methods for producing aptamers that are capable of binding to target molecules and improved photoSELEX methods for producing photoreactive aptamers that are capable of both binding and covalently cross linking to target molecules. Specifically, the present disclosure describes methods for producing aptamers and photoaptamers having slower dissociation rate constants than are obtained using prior SELEX and photoSELEX methods. The disclosure further describes aptamers and photoaptamers having slower dissociation rate constants than those obtained using prior methods. In addition, the disclosure describes aptamer constructs that include a variety of functionalities, including a cleavable element, a detection element, and a capture or immobilization element.

  本公开内容描述了生产能够与目标分子结合的适配体的改进 SELEX 方法，以及生产能够与目标分子结合和共价交联的光活性适配体的改进 photoSELEX 方法。具体地说，本公开内容描述了生产解离速率常数比使用先前的 SELEX 和 photoSELEX 方法更慢的适配体和光适配体的方法。本公开进一步描述了具有比使用先前方法获得的解离速率常数更慢的合体和光合体。此外，本发明还描述了包括各种功能的aptamer构建体，包括可裂解元件、检测元件和捕获或固定元件。
• Improved selex and photoselex
  申请人：Somalogic, Inc.

  公开号：EP2336314A1

  公开（公告）日：2011-06-22

  The present disclosure describes improved SELEX methods for generating nucleic acid ligands that are capable of binding to target molecules and improved photoSELEX methods for generating photoreactive nucleic acid ligands that are capable of both binding and covalently crosslinking to target molecules. The disclosure further describes nucleic acid libraries having expanded physical and chemical properties and their use in SELEX and photoSELEX; methods for increasing the crosslinking efficiencies of photoaptamers; methods for producing photoaptamers having selective modifications that enhance functionality and minimize non-specific photoreactions; and methods for generating truncated nucleic acid ligands from nucleic acid ligands of longer length. The disclosure further describes aptamers and photoaptamers obtained by using any of the foregoing.

  本公开内容描述了生成能够与目标分子结合的核酸配体的改进 SELEX 方法，以及生成能够与目标分子结合并共价交联的光活性核酸配体的改进 photoSELEX 方法。本公开进一步描述了具有更多物理和化学特性的核酸文库及其在 SELEX 和 photoSELEX 中的应用；提高光俘获物交联效率的方法；生产具有选择性修饰的光俘获物的方法，这些修饰可增强功能性并使非特异性光反应最小化；以及从较长的核酸配体生成截短核酸配体的方法。本公开进一步描述了通过使用上述任一方法获得的配合物和光配合物。
• Chemically modified aptamers with improved off-rates
  申请人：Somalogic, Inc.

  公开号：EP2489743A2

  公开（公告）日：2012-08-22

  The present disclosure describes improved SELEX methods for producing aptamers that are capable of binding to target molecules and improved photoSELEX methods for producing photo reactive aptamers that are capable of both binding and covalently cross linking to target molecules. Specifically, the present disclosure describes methods for producing aptamers and photoaptamers having slower dissociation rate constants than are obtained using prior SELEX and photoSELEX methods. The disclosure further describes aptamers and photoaptamers having slower dissociation rate constants than those obtained using prior methods. In addition, the disclosure describes aptamer constructs that include a variety of functionalities, including a cleavable element, a detection element, and a capture or immobilization element.

  本公开内容描述了生产能够与目标分子结合的适配体的改进 SELEX 方法，以及生产能够与目标分子结合和共价交联的光反应适配体的改进 photoSELEX 方法。具体地说，本公开内容描述了生产解离速率常数比使用先前的 SELEX 和 photoSELEX 方法更慢的合体和光合体的方法。本公开进一步描述了具有比使用先前方法获得的解离速率常数更慢的合体和光合体。此外，本发明还描述了包括各种功能的aptamer构建体，包括可裂解元件、检测元件和捕获或固定元件。
• Detection methods using aptamers
  申请人：Somalogic, Inc.

  公开号：EP2613147A1

  公开（公告）日：2013-07-10

  The present disclosure describes methods, devices, reagents, and kits for the detection of one or more target molecules that may be present in a test sample. In one embodiment, a test sample is contacted with an aptamer that includes a tag and has a specific affinity for a target molecule. An aptamer affinity complex that includes an aptamer bound to its target molecule is allowed to form. If the test sample contains the target molecule, an aptamer affinity complex will generally form in the test sample. The aptamer affinity complex is optionally converted to an aptamer covalent complex that includes an aptamer covalently bound to its target molecule. The aptamer affinity complex (or optional aptamer covalent complex) can then be detected and/or quantified using any of a variety of methods known to one skilled in the art, including using a solid support, using mass spectrometry, and using quantitative polymerase chain reaction (Q-PCR).

  本公开介绍了用于检测可能存在于测试样品中的一种或多种目标分子的方法、装置、试剂和试剂盒。在一个实施方案中，测试样品与包括标记并对目标分子具有特异性亲和力的适配体接触。在此过程中，会形成一个包括与目标分子结合的适配体的亲和复合物。如果测试样本中含有目标分子，则通常会在测试样本中形成一个拟合亲和复合物。该亲和复合物可选择性地转化为亲和共价复合物，其中包括与目标分子共价结合的适配体。然后，可以使用本领域技术人员已知的各种方法检测和/或量化适配体亲和复合物（或任选的适配体共价复合物），包括使用固体支持物、质谱法和定量聚合酶链反应（Q-PCR）。