Provided are an isolated polynucleotide and a method for controlling insect invasion. The isolated polynucleotide is a plurality of target sequences for controlling target gene c35112 of a coleopteran pest, Monolepta hieroglyphica, comprising: a) a polynucleotide sequence shown as SEQ ID NO: 1; or (b) a polynucleotide sequence having at least 15 or 17 or 19 or 21 contiguous nucleotides of SEQ ID NO: 1, a double-stranded RNA comprising at least one strand complementary to the polynucleotide sequence being capable of inhibiting the growth of coleopteran pests after being ingested by the coleopteran pests; or (c) any one of polynucleotide sequences shown as SEQ ID NO: 3 to SEQ ID NO: 20; or (d) a polynucleotide sequence that hybridizes under stringent conditions to a polynucleotide sequence as defined in (a), (b) or (c).
本发明提供了一种分离的多核苷酸和一种控制昆虫入侵的方法。分离的多核苷酸是用于控制鞘翅目害虫 Monolepta hieroglyphica 的靶
基因 c35112 的多个靶序列,包括:(a)
SEQ ID NO: 1 所示的多核苷酸序列;或(b)
SEQ ID NO:1的至少一条链互补的双链RNA,该双链RNA在被鞘翅目害虫摄食后能够抑制鞘翅目害虫的生长;或(c)
SEQ ID NO: 3至
SEQ ID NO: 20所示多核苷酸序列中的任何一种;或(d)在严格条件下与(a)、(b)或(c)中定义的多核苷酸序列杂交的多核苷酸序列。