Ald-Ph-amido-PEG1-C2-Pfp ester | 2101206-67-9

• 分子结构分类: 有机化合物 - 苯类化合物 - 苯酚酯
• 英文名称: Ald-Ph-amido-PEG1-C2-Pfp ester
• 英文别名: (2,3,4,5,6-pentafluorophenyl) 3-[2-[(4-formylbenzoyl)amino]ethoxy]propanoate
• CAS: 2101206-67-9
• 化学式: C₁₉H₁₄F₅NO₅
• 分子量: 431.3
• InChiKey: JUOKBCYOAVSJOJ-UHFFFAOYSA-N

计算性质

• 辛醇/水分配系数(LogP)：
  2.4
• 重原子数：
  30
• 可旋转键数：
  10
• 环数：
  2.0
• sp3杂化的碳原子比例：
  0.21
• 拓扑面积：
  81.7
• 氢给体数：
  1
• 氢受体数：
  10

文献信息

• Proximity Assay for In Situ Detection of Targets
  申请人：VENTANA MEDICAL SYSTEMS, INC.

  公开号：US20160002701A1

  公开（公告）日：2016-01-07

  A proximity detection method is described that utilizes enzymatic biotinylation to detect targets in a sample, particularly formalin fixed paraffin embedded samples using automated staining platforms. One disclosed embodiment comprises contacting the sample with a first conjugate comprising a biotin ligase and a first specific binding moiety that binds proximally to the first target; contacting the sample with a second conjugate comprising a biotin ligase substrate and a second specific binding moiety that binds proximally to the second target; subjecting the sample to conditions that allow biotinylation of the biotin ligase substrate by the biotin ligase when the first target and the second target have a proximal arrangement; and detecting biotinylation of the biotin ligase substrate. The conditions that allow biotinylation of the substrate include addition of biotin and ATP. The method also may comprise contacting the sample with a streptavidin-enzyme conjugate. Signal amplification also can be used.
• US9663814B2
  申请人：——

  公开号：US9663814B2

  公开（公告）日：2017-05-30
• [EN] PROXIMITY ASSAY FOR IN SITU DETECTION OF TARGETS<br/>[FR] ESSAI PAR PROXIMITÉ POUR DÉTECTION IN SITU DE CIBLES
  申请人：VENTANA MED SYST INC

  公开号：WO2014139980A1

  公开（公告）日：2014-09-18

  A proximity detection method is described that utilizes enzymatic biotinylation to detect targets in a sample, particularly formalin fixed paraffin embedded samples using automated staining platforms. One disclosed embodiment comprises contacting the sample with a first conjugate comprising a biotin ligase and a first specific binding moiety that binds proximally to the first target; contacting the sample with a second conjugate comprising a biotin ligase substrate and a second specific binding moiety that binds proximally to the second target; subjecting the sample to conditions that allow biotinylation of the biotin ligase substrate by the biotin ligase when the first target and the second target have a proximal arrangement; and detecting biotinylation of the biotin ligase substrate. The conditions that allow biotinylation of the substrate include addition of biotin and ATP. The method also may comprise contacting the sample with a streptavidin-enzyme conjugate. Signal amplification also can be used.