Aklanonic acid | 91432-47-2

• 分子结构分类: 有机化合物 - 苯类化合物 - 蒽
• 英文名称: Aklanonic acid
• 英文别名: 2-[4,5-dihydroxy-3-[(Z)-1-hydroxy-3-oxopent-1-enyl]-9,10-dioxoanthracen-2-yl]acetic acid
• CAS: 91432-47-2
• 化学式: C₂₁H₁₆O₈
• 分子量: 396.3
• InChiKey: WTCFRKORYWVLAQ-ZSOIEALJSA-N

物化性质

• 熔点：
  203-204 °C (decomp)
• 沸点：
  741.5±60.0 °C(Predicted)
• 密度：
  1.552±0.06 g/cm3(Predicted)

计算性质

• 辛醇/水分配系数(LogP)：
  2.7
• 重原子数：
  29
• 可旋转键数：
  5
• 环数：
  3.0
• sp3杂化的碳原子比例：
  0.14
• 拓扑面积：
  149
• 氢给体数：
  4
• 氢受体数：
  8

文献信息

• NOVEL MALONYL-COA BIOSENSOR BASED ON TYPE III POLYKETIDE SYNTHASE, AND USE THEREOF
  申请人：Korea Advanced Institute of Science and Technology

  公开号：EP3608417A1

  公开（公告）日：2020-02-12

  The present invention relates to a recombinant microorganism for malonyl-CoA detection in which a type III polyketide synthase-encoding gene is inserted in the genome or in which a recombinant vector containing the gene is introduced; a method of screening a malonyl-CoA production-inducing substance using the recombinant microorganism; a method of screening a gene which is involved in increased malonyl-CoA production; and a method comprising knocking down the gene, screened by the method, in a microorganism, thus increasing the production of malonyl-CoA in the microorganism, and producing a useful substance in the microorganism using malonyl-CoA as a precursor. The use of the biosensor according to the present invention provides single-step signal generation, utilization in various microorganisms, utilization in self-fluorescent microorganisms, a simple construction method, and a simple screening method. In addition, when the present invention is combined with high-throughput screening, it has advantages in that strains having increased malonyl-CoA producing ability can be screened very easily and rapidly (∼3 days) and can be applied directly to the malonyl-CoA-based production of useful compounds.

  本发明涉及一种用于检测丙二酰-CoA 的重组微生物，在该微生物的基因组中插入了 III 型多酮合成酶编码基因或引入了含有该基因的重组载体；一种使用该重组微生物筛选丙二酰-CoA 生成诱导物质的方法；一种筛选参与丙二酰-CoA 产量增加的基因的方法；以及一种方法，包括在微生物中敲除该方法筛选出的基因，从而增加微生物中丙二酰-CoA 的产量，并以丙二酰-CoA 为前体在微生物中生产有用的物质。根据本发明的生物传感器的使用提供了单步信号生成、在各种微生物中的利用、在自荧光微生物中的利用、简单的构建方法和简单的筛选方法。此外，当本发明与高通量筛选相结合时，其优势在于可以非常容易和快速地（∼3 天）筛选出具有丙二酰-CoA 生成能力的菌株，并可直接应用于以丙二酰-CoA 为基础的有用化合物的生产。
• Processes and host cells for genome, pathway, and biomolecular engineering
  申请人：enEvolv, Inc.

  公开号：US10370654B2

  公开（公告）日：2019-08-06

  The present disclosure provides compositions and methods for genomic engineering.

  本公开提供了基因组工程的组合物和方法。
• Genetically modified host organism for expressing an anthracyclinone analogue, method associated therewith, and synthetic nucleic acids
  申请人：Ferris State University

  公开号：US10980817B2

  公开（公告）日：2021-04-20

  Disclosed is a genetically modified host organism for expressing an anthracyclinone analogue. The genetically modified host organism comprises (i) synthetic nucleic acids; (ii) a biosynthetic pathway encoded by the synthetic nucleic acids, the (ii) biosynthetic pathway comprising a ketosynthase alpha, a ketosynthase beta/chain-length factor, an acyl carrier protein, a 3-oxoacyl-ACP synthase, a propionyl-CoA acyltransferase, a 9-ketoreductase, an aromatase/cyclase, and a second/third-ring cyclase; and (iii) a promoter positioned upstream of and operatively associated with the (ii) biosynthetic pathway. A method and corresponding synthetic nucleic acids are also disclosed.

  本发明公开了一种表达蒽环类类似物的转基因宿主生物。转基因宿主生物包括 (i) 合成核酸；(ii) 由合成核酸编码的生物合成途径，(ii) 生物合成途径包括酮合成酶α、酮合成酶β/链长因子、酰基载体蛋白、3-氧代酰-ACP 合成酶、丙酰-CoA酰基转移酶、9-酮还原酶、芳香酶/环化酶和第二/第三环化酶；和(iii)位于(ii)生物合成途径上游并与(ii)生物合成途径相关的启动子。还公开了一种方法和相应的合成核酸。
• Multi-plasmid method for preparing large libraries of polyketides and non-ribosomal peptides
  申请人：——

  公开号：US20020192756A1

  公开（公告）日：2002-12-19

  A multiple-plasmid system for heterologous expression of polyketides facilitates combinatorial biosynthesis. The method can be extended to any modular polyketide synthase (PKS) or non-ribosomal peptide synthase (NRPS) and has the potential to produce thousands of novel natural products, including ones derived from further modification of the PKS or NRPS products by tailoring enzymes.

  多质粒异源表达聚酮化合物系统促进了组合生物合成。该方法可扩展到任何模块化的多酮肽合成酶（PKS）或非核糖体肽合成酶（NRPS），并有可能生产出成千上万种新型天然产物，包括通过定制酶对 PKS 或 NRPS 产物进行进一步修饰而得到的产物。
• Genes and uses thereof to modulate secondary metabolite biosynthesis
  申请人：Inze G. Dirk

  公开号：US20060041962A1

  公开（公告）日：2006-02-23

  The present invention relates to the use of a genome wide expression profiling technology in combination with the detection of the presence of secondary metabolites of interest to isolate genes that can be used to modulate the production of secondary metabolites in organisms and cell lines derived therefrom.

  本发明涉及将基因组全表达谱分析技术与检测相关次生代谢物的存在相结合，分离出可用于调节生物体及其衍生细胞系次生代谢物生产的基因。