2-(2,5-Dioxopyrrolidin-1-yl)-6-hydrazinylpyridine-3-carboxamide;propan-2-one

• 分子结构分类: 有机化合物 - 有机杂环化合物 - 吡啶及其衍生物
• 英文名称: 2-(2,5-Dioxopyrrolidin-1-yl)-6-hydrazinylpyridine-3-carboxamide;propan-2-one
• 英文别名: 2-(2,5-dioxopyrrolidin-1-yl)-6-hydrazinylpyridine-3-carboxamide;propan-2-one
• 化学式: C13H17N5O4
• 分子量: 307.31
• InChiKey: NVLDMLBFKHOIEE-UHFFFAOYSA-N

计算性质

• 辛醇/水分配系数(LogP)：
  -0.29
• 重原子数：
  22
• 可旋转键数：
  3
• 环数：
  2.0
• sp3杂化的碳原子比例：
  0.31
• 拓扑面积：
  149
• 氢给体数：
  3
• 氢受体数：
  7

文献信息

• Oligonucleotide-mediated quantitative multiplexed immunoassays
  申请人：The University of Chicago

  公开号：US10550421B2

  公开（公告）日：2020-02-04

  Methods and compositions for quantitative immunoassays are provided, in which ligand-conjugated probes are used to label samples and ligand-surfaced microspheres are used as quantitative reference standards. Certain embodiments provide a method of quantitative flow cytometry where ligands are oligonucleotides, and a sample comprising one or more cells is contacted with a hybridized antibody::fluorophore labeled targeting construct to label the cells, and the labeled cells are analyzed. In some embodiments, a population of quantitative labeled oligospheres labeled with the same fluorescent label as the cells is analyzed using the flow cytometer and used to create a quantitative standard curve of cytometer intensity versus molecules fluorescent label per oligosphere event. A standard curve trendline is established and used to determine the molecules of fluorescent label per cellular event for the antigen-positive cell populations. Based on molecules of fluorescent label per cell, the amount of Antibody Binding per Cell (ABC) is quantified.

  提供了用于定量免疫测定的方法和组合物，其中配体结合探针用于标记样品，配体表面微球用作定量参考标准。某些实施方案提供了一种定量流式细胞术方法，其中配体是寡核苷酸，包含一个或多个细胞的样品与杂交抗体：：荧光团标记的靶向构建体接触以标记细胞，并对标记的细胞进行分析。在某些实施方案中，使用流式细胞仪分析与细胞具有相同荧光标记的定量标记寡核细胞群，并用于创建细胞仪强度与每个寡核细胞事件的分子荧光标记的定量标准曲线。建立标准曲线趋势线，用于确定抗原阳性细胞群每个细胞事件的荧光标签分子数。根据每个细胞的荧光标记分子数，量化每个细胞的抗体结合量 (ABC)。
• OLIGONUCLEOTIDE-MEDIATED QUANTITATIVE MULTIPLEXED IMMUNOASSAYS
  申请人：The University of Chicago

  公开号：US20150344937A1

  公开（公告）日：2015-12-03

  Methods and compositions for quantitative immunoassays are provided, in which ligand-conjugated probes are used to label samples and ligand-surfaced microspheres are used as quantitative reference standards. Certain embodiments provide a method of quantitative flow cytometry where ligands are oligonucleotides, and a sample comprising one or more cells is contacted with a hybridized antibody::fluorophore labeled targeting construct to label the cells, and the labeled cells are analyzed. In some embodiments, a population of quantitative labeled oligospheres labeled with the same fluorescent label as the cells is analyzed using the flow cytometer and used to create a quantitative standard curve of cytometer intensity versus molecules fluorescent label per oligosphere event. A standard curve trendline is established and used to determine the molecules of fluorescent label per cellular event for the antigen-positive cell populations. Based on molecules of fluorescent label per cell, the amount of Antibody Binding per Cell (ABC) is quantified.
• US9663818B2
  申请人：——

  公开号：US9663818B2

  公开（公告）日：2017-05-30
• [EN] OLIGONUCLEOTIDE-MEDIATED QUANTITATIVE MULTIPLEXED IMMUNOASSAYS<br/>[FR] IMMUNO-ESSAIS MULTIPLEXÉS QUANTITATIFS FAISANT INTERVENIR DES OLIGONUCLÉOTIDES
  申请人：UNIV CHICAGO

  公开号：WO2013188756A1

  公开（公告）日：2013-12-19

  Methods and compositions for quantitative immunoassays are provided, in which ligand-conjugated probes are used to label samples and ligand-surfaced microspheres are used as quantitative reference standards. Certain embodiments provide a method of quantitative flow cytometry where ligands are oligonucleotides, and a sample comprising one or more cells is contacted with a hybridized antibody: :fluorophore labeled targeting construct to label the cells, and the labeled cells are analyzed. In some embodiments, a population of quantitative labeled oligospheres labeled with the same fluorescent label as the cells is analyzed using the flow cytometer and used to create a quantitative standard curve of cytometer intensity versus molecules fluorescent label per oligosphere event. A standard curve trendline is established and used to determine the molecules of fluorescent label per cellular event for the antigen-positive cell populations. Based on molecules of fluorecent label per cell, the amount of Antibody Binding per Cell (ABC) is quantified.