申请人:Nara Institute of Science and Technology
公开号:EP1541016A1
公开(公告)日:2005-06-15
The invention provides a process for producing a decaffeinated coffee plant by genetic recombination comprising a step for preparing an antisense sequence or an RNAi sequence of a gene coding for an enzyme related to the caffeine biosynthetic pathway and constructing an expression vector for transformation, a step for introducing the obtained expression vector into Agrobacterium, a step for infecting a cell division-activated tissue piece of a coffee plant or a callus or an adventitious embryo induced from a tissue piece of a coffee plant with the Agrobacterium to transform the tissue piece, the callus or the adventitious embryo and a step for obtaining an transformed coffee plant from a transformed tissue piece, a transformed callus or a transformed adventitious embryo so that a coffee plant with low caffeine content can be produced by suppressing expression of a gene coding for an enzyme related to the caffeine biosynthetic pathway by the antisense method or the RNAi method.
本发明提供了一种通过基因重组生产无咖啡因咖啡植物的工艺,包括制备咖啡因生物合成途径相关酶编码基因的反义序列或RNAi序列并构建用于转化的表达载体的步骤、将获得的表达载体导入农杆菌的步骤,用农杆菌感染咖啡植株细胞分裂激活的组织块或咖啡植株组织块诱导的胼胝体或不定胚以转化组织块的步骤、用反义法或 RNAi 法抑制与咖啡因生物合成途径有关的酶的编码基因的表达,从转化的组织块、转化的胼胝体或转化的不定胚中获得转化的咖啡植株,从而生产出咖啡因含量低的咖啡植株。