6-(1,3-二甲基-2,6-二氧代-7H-嘌呤-8-基)己酸 | 5438-78-8

• 分子结构分类: 有机化合物 - 有机杂环化合物 - 咪唑并嘧啶类
• 中文名称: 6-(1,3-二甲基-2,6-二氧代-7H-嘌呤-8-基)己酸
• 英文名称: 6-(1,3-Dimethyl-2,6-dioxo-2,3,6,7-tetrahydro-1h-purin-8-yl)hexanoic acid
• 英文别名: 6-(1,3-dimethyl-2,6-dioxo-7H-purin-8-yl)hexanoic acid
• CAS: 5438-78-8
• 化学式: C₁₃H₁₈N₄O₄
• 分子量: 294.31
• InChiKey: HAKAABXXLDREMT-UHFFFAOYSA-N

计算性质

• 辛醇/水分配系数(LogP)：
  0.7
• 重原子数：
  21
• 可旋转键数：
  6
• 环数：
  2.0
• sp3杂化的碳原子比例：
  0.54
• 拓扑面积：
  107
• 氢给体数：
  2
• 氢受体数：
  5

文献信息

• Stabilization of monoclonal antibody for use in fluorescent polarization techniques
  申请人：ABBOTT LABORATORIES

  公开号：EP0420102A2

  公开（公告）日：1991-04-03

  A method for determining ligands in a sample is disclosed. The method involves mixing with the sample in which the ligand is to be determined a tracer having the formula of Fig. 1 of the attached drawings or a biologically acceptable salt of such a tracer, a monoclonal antibody, and glycerol added as a part of a solution of the monoclonal antibody in which the glycerol is present in an amount sufficient to increase the stability of the monoclonal antibody in the solution, and then determining the amount of tracer bound to the antibody by fluorescence polarization techniques as a measure of the amount of ligand in the sample. In Fig. 1 of the drawings R is a ligand or analog thereof having at least one common epitope with a ligand to be determined so that the ligand to be determined and the ligand or analog thereof of the tracer are both specifically recognizable by a given antibody, and N is an integer from one to ten. The monoclonal antibody used is one which is capable of specifically recognizing both the ligand to be determined and the tracer. Glycerol is used in the monoclonal antibody solution in an amount, usually from about 5 percent to about 20 percent, sufficient to increase the stability of the monoclonal antibody. The optimum glycerol concentration has been found to be about 10%.

  本发明公开了一种测定样品中配体的方法。该方法包括将具有附图 1 所示化学式的示踪剂或这种示踪剂的生物可接受盐、单克隆抗体和甘油与要测定配体的样品混合。然后通过荧光偏振技术测定与抗体结合的示踪剂的量，以此来衡量样品中配体的量。在附图 1 中，R 是配体或其类似物，与待测定的配体至少有一个共同的表位，因此待测定的配体和示踪剂的配体或其类似物都能被给定的抗体特异性识别，N 是一到十的整数。所用的单克隆抗体应能特异性地识别待测配体和示踪剂。甘油在单克隆抗体溶液中的用量通常为 5%到 20%，足以提高单克隆抗体的稳定性。最佳的甘油浓度约为 10%。