1-[3,5-Bis(trifluoromethyl)phenyl]-3-tert-butylthiourea

• 分子结构分类: 有机化合物 - 苯类化合物 - 苯和取代衍生物
• 英文名称: 1-[3,5-Bis(trifluoromethyl)phenyl]-3-tert-butylthiourea
• 化学式: C₁₃H₁₄F₆N₂S
• 分子量: 344.32
• InChiKey: RSNSNNFLPIBEIB-UHFFFAOYSA-N

计算性质

• 辛醇/水分配系数(LogP)：
  4.3
• 重原子数：
  22
• 可旋转键数：
  2
• 环数：
  1.0
• sp3杂化的碳原子比例：
  0.46
• 拓扑面积：
  56.2
• 氢给体数：
  2
• 氢受体数：
  7

文献信息

• HAPTENS, HAPTEN CONJUGATES, COMPOSITIONS THEREOF AND METHOD FOR THEIR PREPARATION AND USE
  申请人：Ventana Medical Systems, Inc.

  公开号：EP3276349A1

  公开（公告）日：2018-01-31

  A method for performing a multiplexed diagnostic assay, such as for two or more different targets in a sample, is described. One embodiment comprised contacting the sample with two or more specific binding moieties that bind specifically to two or more different targets. The two or more specific binding moieties are conjugated to different haptens, and at least one of the haptens is an oxazole, a pyrazole, a thiazole, a nitroaryl compound other than dinitrophenyl, a benzofurazan, a triterpene, a urea, a thiourea, a rotenoid, a coumarin, a cyclolignan, a heterobiaryl, an azo aryl, or a benzodiazepine. The sample is contacted with two or more different anti-hapten antibodies that can be detected separately. The two or more different anti-hapten antibodies may be conjugated to different detectable labels. In particular, in this invention the haptens are a thiazole sulfonamide or a benzofurazan. The linker in the conjugate is a polymer comprising 1 to 15 ethylene glycol units. A kit with a hapten and anti-hapten antibody is also described.

  本文描述了一种进行多重诊断检测的方法，例如针对样品中两个或多个不同靶点的检测。其中一个实施方案包括用两个或两个以上特异性结合分子与样品接触，这些特异性结合分子与两个或两个以上不同的靶标特异性结合。这两种或两种以上特异性结合分子与不同的抗原共轭，其中至少一种抗原是噁唑、吡唑、噻唑、二硝基苯以外的硝基芳基化合物、苯并呋喃、三萜、脲、硫脲、类橙皮甙、香豆素、环木脂素、杂芳基、偶氮芳基或苯并二氮杂卓。样品与两种或两种以上可分别检测的不同抗链肽抗体接触。这两种或两种以上不同的抗链肽抗体可与不同的可检测标签连接。特别是在本发明中，合剂是噻唑磺酰胺或苯并呋喃。共轭物中的连接体是由 1 至 15 个乙二醇单元组成的聚合物。本发明还描述了一种含有合酶和抗合酶抗体的试剂盒。
• POLYMERIC CARRIERS FOR IMMUNOHISTOCHEMISTRY AND IN SITU HYBRIDIZATION
  申请人：Ventana Medical Systems, Inc.

  公开号：EP3561513A1

  公开（公告）日：2019-10-30

  Certain disclosed embodiments of the present invention concern the synthesis, derivatization, conjugation to immunoglobulins and signal amplification based on discrete, relatively short polymers having plural reactive functional groups that react with plural molecules of interest. Reactive functional groups, such as hydrazides, may be derivatized with a variety of detectable labels, particularly haptens. The remaining reactive functional groups may be conjugated directly to a specific binding molecule, such as to the oxidized carbohydrate of the Fc region of the antibody. Disclosed conjugates display large signal amplification as compared to those based on molecules derivatized with single haptens, and are useful for assay methods, particularly multiplexed assays.

  本发明公开的某些实施方案涉及基于离散的、相对较短的聚合物的合成、衍生化、与免疫球蛋白的连接和信号放大，这些聚合物具有多个活性官能团，可与多个相关分子发生反应。反应性官能团（如酰肼）可与各种可检测的标签（尤其是触媒）进行衍生。其余的活性官能团可直接与特定的结合分子（如抗体 Fc 区的氧化碳水化合物）连接。与基于单一触价素衍生的分子相比，所公开的共轭物显示出较大的信号放大率，适用于检测方法，特别是多重检测。
• Polymeric carriers for immunohistochemistry and in situ hybridization
  申请人：Kosmeder Jerry W.

  公开号：US20080305497A1

  公开（公告）日：2008-12-11

  Certain disclosed embodiments of the present invention concern the synthesis, derivatization, conjugation to immunoglobulins and signal amplification based on discrete, relatively short polymers having plural reactive functional groups that react with plural molecules of interest. Reactive functional groups, such as hydrazides, may be derivatized with a variety of detectable labels, particularly haptens. The remaining reactive functional groups may be conjugated directly to a specific binding molecule, such as to the oxidized carbohydrate of the Fc region of the antibody. Disclosed conjugates display large signal amplification as compared to those based on molecules derivatized with single haptens, and are useful for assay methods, particularly multiplexed assays.
• US7985557B2
  申请人：——

  公开号：US7985557B2

  公开（公告）日：2011-07-26