Fmoc-6-aminohexyldiphosphate | 1130959-30-6

• 分子结构分类: 有机化合物 - 苯类化合物 - 芴
• 英文名称: Fmoc-6-aminohexyldiphosphate
• 英文别名: 9H-fluoren-9-ylmethyl N-[6-[hydroxy(phosphonooxy)phosphoryl]oxyhexyl]carbamate
• CAS: 1130959-30-6
• 化学式: C₂₁H₂₇NO₉P₂
• 分子量: 499.394
• InChiKey: XCKQWNZRXOMYJR-UHFFFAOYSA-N

计算性质

• 辛醇/水分配系数(LogP)：
  1.6
• 重原子数：
  33
• 可旋转键数：
  13
• 环数：
  3.0
• sp3杂化的碳原子比例：
  0.38
• 拓扑面积：
  152
• 氢给体数：
  4
• 氢受体数：
  9

反应信息

• 作为产物：
  描述：

  Fmoc-6-aminohexylphosphate bistriethylamine salt 在 N,N'-羰基二咪唑 、 甲醇 作用下， 以 N,N-二甲基甲酰胺 、 甲醇 为溶剂， 反应 20.5h， 生成 Fmoc-6-aminohexyldiphosphate
  参考文献：
  名称：

  Long, Processive Enzymatic DNA Synthesis Using 100% Dye-Labeled Terminal Phosphate-Linked Nucleotides

  摘要：

  We demonstrate the efficient synthesis of DNA with complete replacement of the four deoxyribonucleoside triphosphate (dNTP) substrates with nucleotides carrying fluorescent labels. A different, spectrally separable fluorescent dye suitable for single molecule fluorescence detection was conjugated to each of the four dNTPs via linkage to the terminal phosphate. Using these modified nucleotides, DNA synthesis by phi 29 DNA polymerase was observed to be processive for products thousands of bases in length, with labeled nucleotide affinities and DNA polymerization rates approaching unmodified dNTP levels. Results presented here show the compatibility of these nucleotides for single-molecule, real-time DNA sequencing applications.

  DOI：

  10.1080/15257770802260741

文献信息

• Long, Processive Enzymatic DNA Synthesis Using 100% Dye-Labeled Terminal Phosphate-Linked Nucleotides
  作者：Jonas Korlach、Arek Bibillo、Jeffrey Wegener、Paul Peluso、Thang T. Pham、Insil Park、Sonya Clark、Geoff A. Otto、Stephen W. Turner

  DOI：10.1080/15257770802260741

  日期：2008.8.28

  We demonstrate the efficient synthesis of DNA with complete replacement of the four deoxyribonucleoside triphosphate (dNTP) substrates with nucleotides carrying fluorescent labels. A different, spectrally separable fluorescent dye suitable for single molecule fluorescence detection was conjugated to each of the four dNTPs via linkage to the terminal phosphate. Using these modified nucleotides, DNA synthesis by phi 29 DNA polymerase was observed to be processive for products thousands of bases in length, with labeled nucleotide affinities and DNA polymerization rates approaching unmodified dNTP levels. Results presented here show the compatibility of these nucleotides for single-molecule, real-time DNA sequencing applications.