2-(4-Isothiocyanatophenoxy)-1,3,2lambda~5~-dioxaphosphinan-2-one | 93834-46-9

• 分子结构分类: 有机化合物 - 苯类化合物 - 苯和取代衍生物
• 英文名称: 2-(4-Isothiocyanatophenoxy)-1,3,2lambda~5~-dioxaphosphinan-2-one
• 英文别名: 2-(4-isothiocyanatophenoxy)-1,3,2λ5-dioxaphosphinane 2-oxide
• CAS: 93834-46-9
• 化学式: C₁₀H₁₀NO₄PS
• 分子量: 271.23
• InChiKey: QWAYEUVTOVRCRX-UHFFFAOYSA-N

计算性质

• 辛醇/水分配系数(LogP)：
  2.9
• 重原子数：
  17
• 可旋转键数：
  3
• 环数：
  2.0
• sp3杂化的碳原子比例：
  0.3
• 拓扑面积：
  89.2
• 氢给体数：
  0
• 氢受体数：
  6

文献信息

• Apparatus and method for edman degradation using a microfluidic system
  申请人：West Virginia University Research Corporation

  公开号：US20040175822A1

  公开（公告）日：2004-09-09

  An apparatus and method for Edman degradation using a microfluidic system to identify and characterize peptides is disclosed. A microfluidic device comprises an entrance channel through which a substantially purified polypeptide is accepted, a reaction channel engaging the entrance channel wherein the substantially purified polypeptide is digested, producing a digestion product, a reagent reservoir engaging the reaction channel, the reagent reservoir capable of delivering a reagent to the reaction channel, and an exit channel extending from the reaction channel, wherein the digestion product travels through the exit channel upon leaving the reaction channel. Protein digestion on the device comprises delivering a substantially purified polypeptide to a reaction channel, confining the polypeptide in the reaction channel, digesting the polypeptide in the reaction channel producing a digestion product, and removing the digestion product from the reaction channel, wherein the last two steps are repeated until the polypeptide is substantially digested.

  本发明公开了一种利用微流体系统进行埃德曼降解以鉴定和表征多肽的装置和方法。微流控装置包括一个入口通道，通过该入口通道接收基本纯化的多肽；一个与入口通道相接的反应通道，在该反应通道中消化基本纯化的多肽，产生消化产物；一个与反应通道相接的试剂储存器，该试剂储存器能够将试剂输送到反应通道；以及一个从反应通道延伸出来的出口通道，其中消化产物在离开反应通道后通过出口通道。该装置上的蛋白质消化包括将基本纯化的多肽输送到反应通道，将多肽限制在反应通道中，在反应通道中消化多肽产生消化产物，以及从反应通道中移除消化产物，其中重复后两个步骤，直到多肽基本消化为止。
• Apparatus and method for Edman degradation on a microfluidic device utilizing an electroosmotic flow pump
  申请人：Timperman T. Aaron

  公开号：US20050133371A1

  公开（公告）日：2005-06-23

  The present invention comprises an electroosmotic flow pump with both anion and cation exchange beads packed in separate channels that pump towards an intersection. Combining the two flow streams results in higher flowrates for the pump and allows operation of the pump over a wide pH range. The pump can be used to deliver solutions ranging from a pH of about 2 to about 12. In a preferred embodiment, the electroosmotic pump of the present invention is fabricated on a microfluidic device capable of Edman degradation. In a preferred embodiment of the present invention, the beads are immobilized in the channels using weirs and membranes, eliminating the need for frits. The pump may be comprised of capillaries. Additionally, the electroosmotic flow pump of the present invention may be integrated into an Integrated Microfluidic Proteome Analysis System.

  本发明包括一种电渗流泵，阴离子和阳离子交换珠分别装在不同的通道中，向一个交叉点泵送。将两股流结合在一起可提高泵的流速，使泵在较宽的 pH 值范围内运行。该泵可用于输送 pH 值从约 2 到约 12 的溶液。在一个优选的实施方案中，本发明的电渗泵是在能够进行埃德曼降解的微流体装置上制造的。在本发明的一个优选实施方案中，使用堰和膜将珠子固定在通道中，无需使用熔块。泵可由毛细管组成。此外，本发明的电渗流泵可集成到集成微流体蛋白质组分析系统中。
• Methods and devices for protein assays
  申请人：Chhabra Swapnil

  公开号：US20070231925A1

  公开（公告）日：2007-10-04

  Methods and devices for protein assays based on Edman degradation in microfluidic channels are disclosed herein. As disclosed, the cleaved amino acid residues may be immobilized in an array format and identified by detectable labels, such as antibodies, which specifically bind given amino acid residues. Alternatively, the antibodies are immobilized in an array format and the cleaved amino acids are labeled identified by being bound by the antibodies in the array.
• US7611834B2
  申请人：——

  公开号：US7611834B2

  公开（公告）日：2009-11-03
• [EN] APPARATUS AND METHOD FOR EDMAN DEGRADATION USING A MICROFLUIDIC SYSTEM<br/>[FR] APPAREIL ET PROCEDES DESTINES A LA DEGRADATION D'EDMAN AU MOYEN D'UN SYSTEME MICROFLUIDIQUE
  申请人：UNIV WEST VIRGINIA

  公开号：WO2004059283A2

  公开（公告）日：2004-07-15

  An apparatus and method for Edman degradation using a microfluidic system to identify and characterize peptides is disclosed. A microfluidic device comprises an entrance channel through which a substantially purified polypeptide is accepted, a reaction channel engaging the entrance channel wherein the substantially purified polypeptide is digested, producing a digestion product, a reagent reservoir engaging the reaction channel, the reagent reservoir capable of delivering a reagent to the reaction channel, and an exit channel extending from the reaction channel, wherein the digestion product travels through the exit channel upon leaving the reaction channel. Protein digestion on the device comprises delivering a substantially purified polypeptide to a reaction channel, confining the polypeptide in the reaction channel, digesting the polypeptide in the reaction channel producing a digestion product, and removing the digestion product from the reaction channel, wherein the last two steps are repeated until the polypeptide is substantially digested.