Method for fixing a protein on a pyrrole-based polymer and use thereof for making a sensor
申请人:Roget Andre
公开号:US20060035208A1
公开(公告)日:2006-02-16
The aim of the present invention is to provide a method for attaching a protein to a conductive polymer, that can be used in particular for producing a sensor or a multisensor, for example a biochip. This aim, and also others, are achieved, in accordance with the invention, by means of a method for attaching a protein to a pyrrole polymer, comprising the following steps: coupling of the protein to be attached with pyrrole so as to obtain a first solution of a protein-pyrrole coupling compound, preparation of a second solution of pyrrole not containing the protein, mixing of said first solution with said second solution so as to obtain an electropolymerization solution, electropolymerization of the pyrrole and of the protein coupled to the pyrrole on a conductive support using said electropolymerization solution.
本发明的目的是提供一种将蛋白质附着在导电聚合物上的方法,特别适用于生产传感器或多传感器,例如生物芯片。根据本发明,通过以下步骤实现了该目的以及其他目的:将待附着的蛋白质与吡咯偶联以获得蛋白质-吡咯偶联化合物的第一溶液,制备不含蛋白质的吡咯的第二溶液,将第一溶液与第二溶液混合以获得电聚合溶液,在导电支撑物上使用所述电聚合溶液对吡咯和与吡咯偶联的蛋白质进行电聚合。