It is to provide a method for measuring glycated hemoglobin in a hemoglobin-containing sample, comprising reacting the hemoglobin-containing sample with a proteolytic enzyme in the presence of a surfactant, and then reacting the obtained reaction product with fructosyl peptide oxidase, wherein at least one of the former reaction and the latter reaction is performed in the presence of an isothiazolinone derivative; and measuring the generated hydrogen peroxide. The present invention provides a method for accurately and highly sensitively measuring glycated hemoglobin in a hemoglobin-containing sample without being influenced by hemoglobin.
本发明提供一种测量含血红蛋白样品中糖化血红蛋白的方法,包括在表面活性剂存在下使含血红蛋白样品与蛋白
水解酶反应,然后使得到的反应产物与
果糖基肽氧化酶反应,其中前一反应和后一反应中至少有一个是在
异噻唑啉酮衍
生物存在下进行的;以及测量生成的
过氧化氢。本发明提供了一种不受血红蛋白影响,准确、高灵敏度地测量含血红蛋白样品中糖化血红蛋白的方法。