| 1130959-42-0

• 物质功能分类: 医药中间体 - 原料药中间体
• 分子结构分类: 有机化合物 - 核苷、核苷酸和类似物 - 嘌呤核苷酸
• CAS: 1130959-42-0
• 化学式: C₁₆H₃₁N₆O₁₈P₅
• 分子量: 750.32
• InChiKey: NUXAHQSNJMHCFJ-YNEHKIRRSA-N

反应信息

• 作为产物：
  描述：

  在 三乙胺 作用下， 以 水 为溶剂， 反应 16.0h， 生成
  参考文献：
  名称：

  Long, Processive Enzymatic DNA Synthesis Using 100% Dye-Labeled Terminal Phosphate-Linked Nucleotides

  摘要：

  We demonstrate the efficient synthesis of DNA with complete replacement of the four deoxyribonucleoside triphosphate (dNTP) substrates with nucleotides carrying fluorescent labels. A different, spectrally separable fluorescent dye suitable for single molecule fluorescence detection was conjugated to each of the four dNTPs via linkage to the terminal phosphate. Using these modified nucleotides, DNA synthesis by phi 29 DNA polymerase was observed to be processive for products thousands of bases in length, with labeled nucleotide affinities and DNA polymerization rates approaching unmodified dNTP levels. Results presented here show the compatibility of these nucleotides for single-molecule, real-time DNA sequencing applications.

  DOI：

  10.1080/15257770802260741