Alkaline lipase, method for producing the same, microorganism producing the same and detergent composition containing alkaline lipase
申请人:SHOWA DENKO KABUSHIKI KAISHA
公开号:EP0571982A1
公开(公告)日:1993-12-01
A microorganism belonging to the genus Pseudomonas; an alkaline lipase produced by the microorganism or its mutants; a method of producing the alkaline lipase; and detergents containing the alkaline lipase as an aid, the alkaline lipase having (1) an operative pH of from 4 to 11.5, and an optimum pH of from 7.0 to 9.5, as measured using triolein emulsion as a substrate; (2) an operative temperature of from 10 to 80°C, and an optimum temperature of from 55 to 65°C, as measured using triolein emulsion as a substrate; (3) a molecular weight of 28,000 ± 2,000 as measured by electrophoresis using SDS polyacrylamide; (4) isoelectric point of 4.5 ± 1.5 as measured by isoelectric focusing polyacrylamide gel electrophoresis; and (5) an inhibition of lipase activity by a detergent component of not higher than 50% as measured using sodium linear-alkylbenzenesulfonate as said detergent component.
The alkaline lipase has an improved stability against detergent components such as a surfactant and a protease, and can be blended with a detergent together with a protease. The alkaline lipase is less susceptible to inhibition of lipase activity by a detergent component and can increase washing power of detergents.
一种属于假单胞菌属的微生物;一种由该微生物或其突变体产生的碱性脂肪酶;一种生产该碱性脂肪酶的方法;以及含有该碱性脂肪酶作为助剂的洗涤剂,该碱性脂肪酶具有(1)以三油脂乳液为底物测得的工作pH值为4至11.5,最适pH值为7.0至9.5;(2)以三油脂乳液为底物测得的工作温度为10至80℃,最适温度为55至65℃。5;(2) 操作温度为 10 至 80°C,最适温度为 55 至 65°C;(3) 经 SDS 聚丙烯酰胺电泳测定,分子量为 28,000±2,000 ;(4) 等电点为 4.5±1.5;(5) 以线性烷基苯磺酸钠作为洗涤剂组分测定,洗涤剂组分对脂肪酶活性的抑制率不高于 50%。
碱性脂肪酶对洗涤剂成分(如表面活性剂和蛋白酶)的稳定性更好,可与洗涤剂和蛋白酶混合使用。碱性脂肪酶不易受洗涤剂成分对脂肪酶活性的抑制,可提高洗涤剂的洗涤能力。