5-rhodamine 110 succinimidyl ester

• 分子结构分类: 有机化合物 - 有机杂环化合物 - 苯并吡喃
• 英文名称: 5-rhodamine 110 succinimidyl ester
• 英文别名: 5-R110-SE；5-carboxyrhodamine-green succinimidyl ester；5-Carboxyrhodamine 110 NHS Ester；2-(3-amino-6-iminoxanthen-9-yl)-5-(2,5-dioxopyrrolidin-1-yl)oxycarbonylbenzoic acid
• 化学式: C₂₅H₁₇N₃O₇
• 分子量: 471.426
• InChiKey: OFESYRATOHCHHO-UHFFFAOYSA-N

计算性质

• 辛醇/水分配系数(LogP)：
  1.2
• 重原子数：
  35
• 可旋转键数：
  5
• 环数：
  5.0
• sp3杂化的碳原子比例：
  0.08
• 拓扑面积：
  160
• 氢给体数：
  3
• 氢受体数：
  9

反应信息

• 作为反应物：
  描述：

  5-rhodamine 110 succinimidyl ester 、 N-{3-[4-((S)-2-Amino-2-carboxy-ethyl)-phenyl]-prop-2-ynyl}-6-(6-hydroxy-3-oxo-3H-xanthen-9-yl)-isophthalamic acid 在 N,N-二异丙基乙胺 作用下， 以 二甲基亚砜 为溶剂， 以69%的产率得到
  参考文献：
  名称：

  FLUORESCENCE RESONANCE ENERGY TRANSFER DYE NUCLEOTIDE TERMINATORS: A NEW SYNTHETIC APPROACH FOR HIGH-THROUGHOUT DNA SEQUENCING

  摘要：

  Fluorescence resonance energy transfer (FRET) based dye-nucleotide terminators (10-13) were designed, synthesized, and formulated with Thermo Sequenase(TM) II DNA polymerase into a robust kit for high throughput DNA sequencing. The key energy transfer (ET) rigid and linear linker (2), required for the syntheses of energy transfer cassettes (6-9) was synthesized via Heck coupling reaction on t-Boc-L-4-iodo-phenylalanine (1) with N-TFA-propargylamine.

  DOI：

  10.1081/ncn-100002307

文献信息

• WO2006/114409
  申请人：——

  公开号：——

  公开（公告）日：——
• Unnatural amino acid derived FRET cassettes, terminators and their DNA sequencing potential
  作者：Satyam Nampalli、Weihong Zhang、T Sudhakar Rao、Haiguang Xiao、Lakshmi P Kotra、Shiv Kumar

  DOI：10.1016/s0040-4039(02)00196-x

  日期：2002.3

  An unnatural, tri-functional amino acid. t-BOC-L-para-amino-phenylalanine (1). was utilized in the design and syntheses of highly efficient FRET cassettes (4-7) and converted them into 2'.3'-dideoxynucleotide terminators (8-11) for investigating DNA sequencing potential coupled with a thermostable DNA polymerase. Thermo Sequenase(TM) II . (C) 2002 Elsevier Science Ltd. All rights reserved.
• FLUORESCENT LABELING OF LIVING CELLS
  申请人：Empire Technology Development LLC

  公开号：US20140206840A1

  公开（公告）日：2014-07-24

  Latent fluorescent tags, including compounds of Formula I, methods of making latent fluorescent tags, and methods of fluorescently labeling living cells are provided. The compounds of Formula I have the structure: wherein each of the variables are as defined herein.