1-Formyl-2-dansylhydrazin | 64755-27-7

• 分子结构分类: 有机化合物 - 苯类化合物 - 萘
• 英文名称: 1-Formyl-2-dansylhydrazin
• 英文别名: N-[[5-(dimethylamino)naphthalen-1-yl]sulfonylamino]formamide
• CAS: 64755-27-7
• 化学式: C₁₃H₁₅N₃O₃S
• 分子量: 293.346
• InChiKey: BBOKQLQFNGQFDL-UHFFFAOYSA-N

计算性质

• 辛醇/水分配系数(LogP)：
  0.85
• 重原子数：
  20.0
• 可旋转键数：
  5.0
• 环数：
  2.0
• sp3杂化的碳原子比例：
  0.15
• 拓扑面积：
  78.51
• 氢给体数：
  2.0
• 氢受体数：
  4.0

反应信息

• 作为产物：
  描述：

  丹酰氯 、 甲酰肼 以 乙酸乙酯 为溶剂， 生成 1-Formyl-2-dansylhydrazin
  参考文献：
  名称：

  Purification and Characterization of Animal Porphobilinogen Synthases. I. Bovine Liver Porphobilinogen Synthase

  摘要：

  Porphobilinogen synthase was purified from ox liver by ammonium sulfate fractionation, heat denaturation and column chromatography (purification: 400-fold; specific activity 4.72 nkat). The molecular weight of the native enzyme obtained by thin-layer gel filtration is about 280 000. Using 8M urea in the presence of dithiothreitol as reducing agent, the molecule breaks down into 8 subunits of molecular weight 36 000 (dodecylsulfate gel electrophoresis); the preparation of aminoethylated subunit is described. According to the above-mentioned molecular weight and to the above-mentioned molecular weight and to the quantitative amino acid analysis after total hydrolysis, the following compositon of the enzymes subunit was calculated ASX23-25 Thr7 Ser23-24 Glx29-31 Pro22-23 Gly22-24 Ala36-37 Val23-26 Met7 Ile9 Leu34-35 Tyr10 Phe11-12 Lys11-12 Cys6-7 His6-8 Arg22 Trp1-2. The subunits, having two free sulfhydryl groups, therefore consists of a chain of about 306 amino acids. The Dansyl-Edman procedure did not enable identification of any free N-terminal amino acid. The acyl group blocking the N-terminus is an acetyl group. It was identified, after hydrazinolysis of the enzyme, by means of chromatographic comparison with 1-formyl-2-dansyl-hydrazine and 1-acetyl-2-dansylhydrazine, whose syntheses and UV spectra are described.

  DOI：

  10.1515/bchm2.1977.358.2.1081