申请人:Wako Pure Chemical Industries, Ltd.
公开号:EP3235876A1
公开(公告)日:2017-10-25
Conventional quenchers do not have quenching ability enough to suppress fluorescence emission of compounds having fluorescent property. Accordingly, it is an object of the present invention to provide a quencher capable of sufficiently quenching fluorescence of the compound having fluorescent property, including a xanthene-based dye.
The present invention relates to a quencher, or the like, comprising a compound represented by the following general formula (1):
[wherein R5 each independently represent a halogen atom, an alkyl group, an alkoxy group, an alkylthio group, an amino group having a substituent or not having a substituent, a hydroxy group, an aryl group, an aryloxy group, or an arylalkyl group; R6 represents a group having a polymerizable unsaturated group, a hydroxy group, or the like; Y1 represents an oxygen atom, or the like; An- represents an anion; Ar1 represents a specific ring structure; * and ** represent binding positions; Ar2 represents a benzene ring, a naphthalene ring, or an anthracene ring; n1 represents a specific integer;
and the following structure (1-10) in the general formula (1) is an asymmetric structure;
(wherein R5, Y1, Ar1, Ar2, n1, * and ** are the same as described above.).].
传统淬灭剂的淬灭能力不足以抑制具有荧光特性的化合物的荧光发射。因此,本发明的目的是提供一种淬灭剂,能够充分淬灭具有荧光特性的化合物的荧光,包括呫吨基染料。
本发明涉及一种淬灭剂或类似物,其中包含由以下通式(1)表示的化合物:
[其中 R5 各自独立地代表卤原子、烷基、烷氧基、烷硫基、具有取代基或不具有取代基的氨基、羟基、芳基、芳氧基或芳烷基;R6 代表具有可聚合的不饱和基团、羟基或类似基团;Y1 代表氧原子或类似物; An- 代表阴离子; Ar1 代表特定的环结构; * 和 ** 代表结合位置; Ar2 代表苯环、萘环或蒽环; n1 代表特定的整数;
通式(1)中的以下结构(1-10)为不对称结构;
(其中 R5、Y1、Ar1、Ar2、n1、* 和 ** 与上述相同)]。