guanosine 2',5'-bis(monophosphate) | 3184-69-8

• 分子结构分类: 有机化合物 - 核苷、核苷酸和类似物 - 嘌呤核苷酸
• 英文名称: guanosine 2',5'-bis(monophosphate)
• 英文别名: guanosine 3',5'-diphosphate；pGp；Guanosine 2',5'-bisphosphate；[(2R,3R,4R,5R)-2-(2-amino-6-oxo-1H-purin-9-yl)-4-hydroxy-5-(phosphonooxymethyl)oxolan-3-yl] dihydrogen phosphate
• CAS: 3184-69-8
• 化学式: C₁₀H₁₅N₅O₁₁P₂
• 分子量: 443.204
• InChiKey: DATWFCNMAUNLSZ-UUOKFMHZSA-N

物化性质

• 密度：
  2.63±0.1 g/cm3(Predicted)

计算性质

• 辛醇/水分配系数(LogP)：
  -4.6
• 重原子数：
  28
• 可旋转键数：
  6
• 环数：
  3.0
• sp3杂化的碳原子比例：
  0.5
• 拓扑面积：
  248
• 氢给体数：
  7
• 氢受体数：
  13

反应信息

• 作为产物：
  描述：

  鸟苷酸 在 sodium hydroxide 、 Sodium trimetaphosphate 作用下， 以 水 为溶剂， 反应 672.0h， 生成 guanosine 2',5'-bis(monophosphate) 、 Guanosin-3',5'-diphosphat
  参考文献：
  名称：

  核苷酸与无机环三磷酸的磷酸化

  摘要：

  在各种条件下（P3m 与核苷酸的混合比、pH 值）在水溶液中研究了核苷酸（核苷 3'-和 5'-单磷酸酯，以及 2'-脱氧核苷 5'-单磷酸酯）与无机环三磷酸钠 (P3m) 的磷酸化、反应温度和时间）。(I) 未受保护的 5'-单磷酸核苷 (5'-NMP's) 很容易在顺式 2',3'-二醇处被 P3m 磷酸化，形成选择性核苷 2',5'-双（单磷酸）（2',5 '-NDP's）、核苷 3',5'-双（单磷酸）（3',5'-NDP）和核苷 2',3'-环状 5'-双（单磷酸）（cNDP）。(2) 5'-NMP's 的磷酸化强烈依赖于混合比、pH、反应温度和时间。在 P3m 与 5'-NMP's 的高混合比 (5:1–10:1)、高 pH (12) 和室温条件下，92-98% 的 5'-NMP's 转化为 3',5' -NDP 和 2'，大致等摩尔量的 5'-NDP。(3) 在 5'-NMP's

  DOI：

  10.1246/bcsj.64.490

文献信息

• Enzymatic and Molecular Characterization of Arabidopsis ppGpp Pyrophosphohydrolase, AtNUDX26
  作者：Daisuke ITO、Takahiro KATO、Takanori MARUTA、Masahiro TAMOI、Kazuya YOSHIMURA、Shigeru SHIGEOKA

  DOI：10.1271/bbb.120523

  日期：2012.12.23

  Not only in bacteria but also in plant cells, guanosine-3',5'-tetraphosphate (ppGpp) is an important signaling molecule, that affects various cellular processes. In this study, we identified nucleoside diphosphates linked to some moiety X (Nudix) hydrolases, AtNUDX11, 15, 25, and 26, having ppGpp pyrophosphohydrolase activity from Arabidopsis plants. Among these, AtNUDX26 localized in chloroplasts had the highest V-max and k(cat) values, leading to high catalytic efficiency, k(cat)/K-m. The activity of AtNUDX26 required Mg2+ or Mn2+ ions as cofactor and was optimal at pH 9.0 and 50 degrees C. The expression of AtNUDX26 and of ppGpp metabolism-associated genes was regulated by various types of stress, suggesting that AtNUDX26 regulates cellular ppGpp levels in response to stress and impacts gene expression in chloroplasts. This is the first report on the molecular properties of ppGpp pyrophosphohydrolases in plants.
• Double-Stranded Olidonucleotides and Uses Therefor
  申请人：Dixon Nicholas Edward

  公开号：US20090286696A1

  公开（公告）日：2009-11-19

  The present invention relates generally to the field of screening or diagnostic applications in which a target is required to be displayed for binding to another molecule, or interaction or reaction with another molecule. In particular, the present invention relates to the use of DNA/protein interactions to immobilize or present one or more biomolecules for screening purposes. The present invention more particularly relates to double-stranded oligonucleotides, wherein said oligonucleotide comprises a first strand and a second strand, wherein: (a) said first strand comprises the sequence: 5′-N C R N D G T T G T A A C N D A-3′ (SEQ ID NO: 1) or an analogue or derivative of said sequence; and (b) said second strand comprises the sequence: 5′-T N D G T T A C A A C N D T N C -3′ (SEQ ID NO: 2) or an analogue or derivative of said sequence wherein R is a purine, N C and N D are each a DNA or RNA residue or analogue thereof, N D residues in said first strand and said second strand are sufficiently complementary to permit said N D residues to be annealed in the double-stranded oligonucleotide, and the sequence 5′-GTTGTAAC-3′ (SEQ ID NO: 3) of said first strand is annealed to the complementary sequence 5′GTTACAAC-3′ (SEQ ID NO: 4) of said second strand.
• DIAGNOSTICS IN A MONOPLEX/MULTIPLEX FORMAT
  申请人：Dixon Nicholas Edward

  公开号：US20110189664A1

  公开（公告）日：2011-08-04

  The present invention relates to a method of detecting and/or quantifying a target molecule from a sample obtained from a subject wherein the method comprises: (i) incubating a fusion protein or conjugate comprising a Ter binding polypeptide fused to at least one anti-target molecule or fragment thereof with a partially double-stranded oligonucleotide for a time and under conditions sufficient to bind to said Ter binding polypeptide thereby producing a complex; (ii) incubating said complex in the presence of said sample comprising said target molecule for a time and under conditions sufficient for said anti-target molecule to bind to said target molecule thereby producing a target-bound complex; (iii) incubating said target-bound complex in the presence of at least one immobilised molecule wherein said immobilised molecule has an affinity to said target molecule; (iv) incubating said immobilised molecule for a time and under conditions sufficient to bind to said target molecule thus immobilising said target molecule; and (v) detecting and/or quantifying said target molecule.
• [EN] DIAGNOSTICS IN A MONOPLEX/MULTIPLEX FORMAT<br/>[FR] DIAGNOSTICS DANS UN FORMAT MONOPLEX/MULTIPLEX
  申请人：UNIV WOLLONGONG

  公开号：WO2008148143A1

  公开（公告）日：2008-12-11

  [EN] The present invention relates to a method of detecting and/or quantifying a target molecule from a sample obtained from a subject wherein the method comprises: (i) incubating a fusion protein or conjugate comprising a Ter binding polypeptide fused to at least one anti-target molecule or fragment thereof with a partially double-stranded oligonucleotide for a time and under conditions sufficient to bind to said Ter binding polypeptide thereby producing a complex; (ii) incubating said complex in the presence of said sample comprising said target molecule for a time and under conditions sufficient for said anti-target molecule to bind to said target molecule thereby producing a target-bound complex; (iii) incubating said target-bound complex in the presence of at least one immobilised molecule wherein said immobilised molecule has an affinity to said target molecule; (iv) incubating said immobilised molecule for a time and under conditions sufficient to bind to said target molecule thus immobilising said target molecule; and (v) detecting and/or quantifying said target molecule.
  [FR] La présente invention concerne un procédé de détection et/ou de quantification d'une molécule cible dans un échantillon prélevé sur un sujet. Le procédé comprend : (i) l'incubation d'une protéine de fusion ou d'un conjugué comprenant un polypeptide de liaison Ter fusionné à au moins une molécule anti-cible ou à l'un de ses fragments avec un oligonucléotide partiellement bicaténaire pendant une durée et dans des conditions suffisantes pour lier le polypeptide de liaison Ter, ce qui produit complexe; (ii) l'incubation du complexe en présence de l'échantillon comprenant la molécule cible pendant une durée et dans des conditions suffisantes pour que la molécule anti-cible se lie à la molécule cible, ce qui produit un complexe lié à la cible; (iii) l'incubation du complexe lié à la cible en présence d'au moins une molécule immobilisée, cette dernière ayant une affinité avec la molécule cible; (iv) l'incubation de la molécule immobilisée pendant une durée et dans des conditions suffisantes pour lier la molécule cible, ce qui immobilise ladite molécule cible; et (v) la détection et/ou la quantification de la molécule cible.
• Phosphorylation of Nucleotides with Inorganic Cyclo-Triphosphate
  作者：Mitsutomo Tsuhako、Rumi Kunitomi、Yoshinobu Baba、Tohru Miyajima

  DOI：10.1246/bcsj.64.490

  日期：1991.2

  5′-bis(monophosphate) (2′,5′-NDP’s), nucleoside 3′,5′-bis(monophosphate) (3′,5′-NDP’s), and nucleoside 2′,3′-cyclic 5′-bis(monophosphate) (cNDP’s). (2) The phosphorylation of 5′-NMP’s was strongly dependent on mixing ratio, pH, reaction temperature, and time. Under conditions of high mixing ratios of P3m to 5′-NMP’s (5:1–10:1), high pH (12), and room temperature, 92–98% of 5′-NMP’s was converted into 3′,5′-NDP’s and

  在各种条件下（P3m 与核苷酸的混合比、pH 值）在水溶液中研究了核苷酸（核苷 3'-和 5'-单磷酸酯，以及 2'-脱氧核苷 5'-单磷酸酯）与无机环三磷酸钠 (P3m) 的磷酸化、反应温度和时间）。(I) 未受保护的 5'-单磷酸核苷 (5'-NMP's) 很容易在顺式 2',3'-二醇处被 P3m 磷酸化，形成选择性核苷 2',5'-双（单磷酸）（2',5 '-NDP's）、核苷 3',5'-双（单磷酸）（3',5'-NDP）和核苷 2',3'-环状 5'-双（单磷酸）（cNDP）。(2) 5'-NMP's 的磷酸化强烈依赖于混合比、pH、反应温度和时间。在 P3m 与 5'-NMP's 的高混合比 (5:1–10:1)、高 pH (12) 和室温条件下，92-98% 的 5'-NMP's 转化为 3',5' -NDP 和 2'，大致等摩尔量的 5'-NDP。(3) 在 5'-NMP's