5′-tyrosyl adenylate | 50466-77-8

• 分子结构分类: 有机化合物 - 核苷、核苷酸和类似物 - 嘌呤核苷酸
• 英文名称: 5′-tyrosyl adenylate
• 英文别名: Tyrosyladenylat；[5']adenylic L-tyrosine anhydride；[5']adenylic acid L-tyrosine anhydride；[5']Adenylsaeure-L-tyrosin-anhydrid；5'-O-[(R)-{[(2s)-2-Amino-3-(4-Hydroxyphenyl)propanoyl]oxy}(Hydroxy)phosphoryl]adenosine；[[(2R,3S,4R,5R)-5-(6-aminopurin-9-yl)-3,4-dihydroxyoxolan-2-yl]methoxy-hydroxyphosphoryl] (2S)-2-amino-3-(4-hydroxyphenyl)propanoate
• CAS: 50466-77-8
• 化学式: C₁₉H₂₃N₆O₉P
• 分子量: 510.4
• InChiKey: NFCVQVDMLQEZAK-URQYDQELSA-N

计算性质

• 辛醇/水分配系数(LogP)：
  -4.5
• 重原子数：
  35
• 可旋转键数：
  9
• 环数：
  4.0
• sp3杂化的碳原子比例：
  0.37
• 拓扑面积：
  238
• 氢给体数：
  6
• 氢受体数：
  14

反应信息

• 作为产物：
  描述：

  [5']adenylic acid-(N-benzyloxycarbonyl-L-tyrosine)-anhydride 在 钯 、 溶剂黄146 作用下， 生成 5′-tyrosyl adenylate
  参考文献：
  名称：

  Moldave et al., Journal of Biological Chemistry, 1959, vol. 234, p. 841,843

  摘要：

  DOI：

文献信息

• A bisubstrate reagent orchestrating adenosine triphosphate and<scp>l</scp>-tyrosine and making tyrosyl adenylate: partial mimicking of tyrosyl-tRNA synthetase
  作者：Harpreet Singh、Baljit Kaur、Harpreet Kaur、Palwinder Singh

  DOI：10.1039/c8ob02866d

  日期：——

  Mimicking the catalytic mode of aminoacyl-tRNA synthetase, the mode of action of reagent1was orchestrated for the formation of 5′-aminoacyl adenylate.

  模仿氨酰-tRNA合成酶的催化模式，试剂1的作用方式被策划用于形成5'-氨酰腺苷酸。
• In vivo incorporation of unnatural amino acids
  申请人：Schultz Peter

  公开号：US20050250183A1

  公开（公告）日：2005-11-10

  The invention provides methods and compositions for in vivo incorporation of unnatural amino acids. Also provided are compositions including proteins with unnatural amino acids.

  本发明提供了在体内合成非天然氨基酸的方法和组合物。同时还提供了包括非天然氨基酸蛋白质的组合物。
• IN Vivo Incorporation of Unnatural Amino Acids
  申请人：SCHULTZ PETER G.

  公开号：US20120202243A1

  公开（公告）日：2012-08-09

  The invention provides methods and compositions for in vivo incorporation of unnatural amino acids. Also provided are compositions including proteins with unnatural amino acids.

  该发明提供了体内合成非天然氨基酸的方法和组合物。还提供了含有非天然氨基酸蛋白质的组合物。
• Methods and composition for the production of orthoganal tRNA-Aminoacyl-tRNA synthetase pairs
  申请人：The Scripps Research Institute

  公开号：EP2128246A1

  公开（公告）日：2009-12-02

  This invention provides compositions and methods for generating components of protein biosynthetic machinery including orthogonal tRNAs, orthogonal aminoacyl-tRNA synthetases, and orthogonal pairs of tRNAs/synthetases. Methods for identifying orthogonal pairs are also provided. These components can be used to incorporate unnatural amino acids into proteins in vivo.

  本发明提供了用于生成蛋白质生物合成机制成分的组合物和方法，这些成分包括正交 tRNA、正交氨基酰-tRNA 合成酶和正交对 tRNA/合成酶。还提供了识别正交对的方法。这些成分可用于在体内将非天然氨基酸结合到蛋白质中。
• Site specific incorporation of heavy atom-containing unnatural amino acids into proteins for structure determination
  申请人：Xie Jianming

  公开号：US20050272121A1

  公开（公告）日：2005-12-08

  Translation systems and other compositions including orthogonal aminoacyl tRNA-synthetases that preferentially charge an orthogonal tRNA with an iodinated or brominated amino acid are provided. Nucleic acids encoding such synthetases are also described, as are methods and kits for producing proteins including heavy atom-containing amino acids, e.g., brominated or iodinated amino acids. Methods of determining the structure of a protein, e.g., a protein into which a heavy atom has been site-specifically incorporated through use of an orthogonal tRNA/aminoacyl tRNA-synthetase pair, are also described.

  提供了包括正交氨基酰 tRNA 合成酶的翻译系统和其他组合物，该合成酶可优先将碘化或溴化氨基酸充入正交 tRNA。 还描述了编码这种合成酶的核酸，以及生产包括含重金属原子的氨基酸（如溴化或碘化氨基酸）的蛋白质的方法和试剂盒。 还描述了确定蛋白质结构的方法，例如，通过使用正交 tRNA/氨基酰 tRNA-合成酶对特异性加入重原子的蛋白质。