申请人:Jones, Mark Howard
公开号:EP0962773A1
公开(公告)日:1999-12-08
A method for quantitating or detecting an analyte using an electrochemical reaction, comprising a material in contact with an electrolyte in a cell or chamber but not in electronic contact with an external electronic circuit, at least some of said material having at least its surface conducting or semi-conducting, and applying a voltage gradient across at least a portion of said material, the size of said material, the conductivity of said electrolyte and/or reactant liquid and the voltage gradient being such that not only are anodic and cathodic faces established in respect of at least some of the conducting or semi-conducting material, rendering said material bipolar, the electropotentials so generated are such that said electrochemical reaction takes place on at least a portion of the surfaces of said material resulting in the production of a detectable signal or detectable material. The said bipolar material which, advantageously, are spherical or fibres, may comprise solid conducting or semi-conducting material or cores of non-conducting material with coatings of conducting or non-conducting material thereon. The material, which are preferably in suspension, may also be bound or captured directly or indirectly onto a surface or filter matrix. The said surface or filter matrix if conducting is not acting as an electrode to carry out electrochemistry. The detection of the electrochemically-generated signal or material is related to the quantity or presence of said analyte.
一种利用电化学反应定量或检测分析物的方法,包括在电池或反应室中与电解液接触但不与外部电子电路接触的材料,所述材料的至少部分至少表面导电或半导电,在所述材料的至少部分上施加电压梯度,所述材料的尺寸、所述电解质和/或反应液的导电性以及电压梯度不仅能在至少部分导电或半导电材料上形成阳极和阴极面,使所述材料成为双极材料,而且所产生的电势能使所述电化学反应在所述材料的至少部分表面上发生,从而产生可检测信号或可检测材料。上述双极材料最好是球状或纤维状,可包括固体导电或半导电材料,或带有导电或非导电材料涂层的非导电材料芯。这些材料最好处于悬浮状态,也可以直接或间接地结合或捕捉到表面或过滤基质上。上述表面或过滤基质如果导电,则不作为进行电化学反应的电极。电化学产生的信号或材料的检测与所述分析物的数量或存在有关。