(E)-tert-butyl-[7-(1-ethoxy-ethoxy)-hept-5-enyloxy]-dimethyl-silane | 1252592-99-6

• 分子结构分类: 有机化合物 - 有机金属化合物 - 有机类金属化合物
• 英文名称: (E)-tert-butyl-[7-(1-ethoxy-ethoxy)-hept-5-enyloxy]-dimethyl-silane
• 英文别名: tert-butyl-[(E)-7-(2-ethoxyethoxy)hept-5-enoxy]-dimethylsilane
• CAS: 1252592-99-6
• 化学式: C₁₇H₃₆O₃Si
• 分子量: 316.557
• InChiKey: DJXGPNKXTRZCHA-PKNBQFBNSA-N

计算性质

• 辛醇/水分配系数(LogP)：
  4.79
• 重原子数：
  21.0
• 可旋转键数：
  12.0
• 环数：
  0.0
• sp3杂化的碳原子比例：
  0.88
• 拓扑面积：
  27.69
• 氢给体数：
  0.0
• 氢受体数：
  3.0

反应信息

• 作为反应物：
  描述：

  (E)-tert-butyl-[7-(1-ethoxy-ethoxy)-hept-5-enyloxy]-dimethyl-silane 在 四溴化碳 、 四丁基氟化铵 、 氨 、 4-甲基苯磺酸吡啶 、 三乙胺 、 三苯基膦 、 sodium hydroxide 作用下， 以 四氢呋喃 、 甲醇 、 乙醇 、 二氯甲烷 、 N,N-二甲基甲酰胺 为溶剂， 反应 44.25h， 生成 [5-(6-amino-purin-9-yl)-3-(7-azido-hept-2-enyloxy)-tetrahydro-furan-2-yl]-methanol
  参考文献：
  名称：

  Synthesis of 3′-O-fluorescently mono-modified reversible terminators and their uses in sequencing-by-synthesis

  摘要：

  Next-generation sequencing (NGS) technologies recently developed are now used for study of genomes from various organisms. Sequencing-by-synthesis (SBS) is a key strategy in the NGS. The SBS uses nucleotides so-called dual-modified reversible terminators (DRTs) in which bases are labeled with fluorophores and 3'-OH is protected with a reversibly cleavable chemical group, respectively. In this study, we examined the possibility of performing SBS with mono-modified reversible terminators (MRTs), in which the reversible blocking group on the 3'-OH plays a dual role as a fluorescent signal report as well as a chemical protection. We studied cyclic reversible termination by using two MRTs (dA and dT), wherein the modifications were two different fluorophores and cleavable to regenerate a free 3'-OH. We here demonstrated that SBS could be achieved with incorporation of MRTs by a DNA polymerase and correct base-calls based on the two different colors from the fluorophores. (C) 2013 Elsevier Ltd. All rights reserved.

  DOI：

  10.1016/j.bmcl.2013.11.040
• 作为产物：
  描述：

  5-((叔丁基二甲基甲硅烷基)氧基)戊醛 在 4-甲基苯磺酸吡啶 、 sodium hydride 、 二异丁基氢化铝 作用下， 以 四氢呋喃 、 二氯甲烷 、 甲苯 、 mineral oil 为溶剂， 反应 16.0h， 生成 (E)-tert-butyl-[7-(1-ethoxy-ethoxy)-hept-5-enyloxy]-dimethyl-silane
  参考文献：
  名称：

  Synthesis of 3′-O-fluorescently mono-modified reversible terminators and their uses in sequencing-by-synthesis

  摘要：

  Next-generation sequencing (NGS) technologies recently developed are now used for study of genomes from various organisms. Sequencing-by-synthesis (SBS) is a key strategy in the NGS. The SBS uses nucleotides so-called dual-modified reversible terminators (DRTs) in which bases are labeled with fluorophores and 3'-OH is protected with a reversibly cleavable chemical group, respectively. In this study, we examined the possibility of performing SBS with mono-modified reversible terminators (MRTs), in which the reversible blocking group on the 3'-OH plays a dual role as a fluorescent signal report as well as a chemical protection. We studied cyclic reversible termination by using two MRTs (dA and dT), wherein the modifications were two different fluorophores and cleavable to regenerate a free 3'-OH. We here demonstrated that SBS could be achieved with incorporation of MRTs by a DNA polymerase and correct base-calls based on the two different colors from the fluorophores. (C) 2013 Elsevier Ltd. All rights reserved.

  DOI：

  10.1016/j.bmcl.2013.11.040

文献信息

• Synthesis of 3′-O-fluorescently mono-modified reversible terminators and their uses in sequencing-by-synthesis
  作者：Da-Rae Kim、Taek-Soo Kim、Eunsun Kim、Sun-Joon Min、Dongyun Shin、Dae-Ro Ahn

  DOI：10.1016/j.bmcl.2013.11.040

  日期：2014.1

  Next-generation sequencing (NGS) technologies recently developed are now used for study of genomes from various organisms. Sequencing-by-synthesis (SBS) is a key strategy in the NGS. The SBS uses nucleotides so-called dual-modified reversible terminators (DRTs) in which bases are labeled with fluorophores and 3'-OH is protected with a reversibly cleavable chemical group, respectively. In this study, we examined the possibility of performing SBS with mono-modified reversible terminators (MRTs), in which the reversible blocking group on the 3'-OH plays a dual role as a fluorescent signal report as well as a chemical protection. We studied cyclic reversible termination by using two MRTs (dA and dT), wherein the modifications were two different fluorophores and cleavable to regenerate a free 3'-OH. We here demonstrated that SBS could be achieved with incorporation of MRTs by a DNA polymerase and correct base-calls based on the two different colors from the fluorophores. (C) 2013 Elsevier Ltd. All rights reserved.