1-[(2R,4S,5R)-4-hydroxy-5-[2-(2-nitrophenyl)propoxycarbothioyloxymethyl]oxolan-2-yl]-5-methylpyrimidine-2,4-dione

• 分子结构分类: 有机化合物 - 核苷、核苷酸和类似物 - 嘧啶核苷
• 英文名称: 1-[(2R,4S,5R)-4-hydroxy-5-[2-(2-nitrophenyl)propoxycarbothioyloxymethyl]oxolan-2-yl]-5-methylpyrimidine-2,4-dione
• 化学式: C20H23N3O8S
• 分子量: 465.5
• InChiKey: QKTWREYTVPOLJV-GUPZPADCSA-N

计算性质

• 辛醇/水分配系数(LogP)：
  2
• 重原子数：
  32
• 可旋转键数：
  8
• 环数：
  3.0
• sp3杂化的碳原子比例：
  0.45
• 拓扑面积：
  175
• 氢给体数：
  2
• 氢受体数：
  9

文献信息

• Method for photolytically deprotecting immobilized nucleoside derivatives, especially in the production of DNA chips
  申请人：NIGU CHEMIE GMBH

  公开号：US20020053508A1

  公开（公告）日：2002-05-09

  The invention relates to a method for the specific photolytic deprotection of nucleoside derivatives that are immobilized on a substrate, especially for use in the production of DNA chips. Said method is characterized in that a gel or viscous liquid layer is applied on the nucleoside derivatives that are immobilized on a substrate. Said gel or viscous liquid contains one or more polymer compounds and at least one representative from the group comprising water, water/C 1 -C 4 alcohol mixtures and polar aprotic solvents. For initiating the deprotection, the nucleoside derivates are irradiated. This method favors a rapid, clean and complete removal of the photolabile protective groups from the nucleoside derivatives, which results in the required purity of the synthesized nucleotide or oligonucleotide sequences.

  本发明涉及一种对固定在基底上的核苷衍生物进行特定光解保护的方法，特别是用于生产 DNA 芯片。该方法的特点是在固定在基底上的核苷衍生物上涂敷一层凝胶或粘稠液体。所述凝胶或粘稠液体含有一种或多种高分子化合物，以及至少一种由水、水/C 1 -C 4 醇混合物和极性钝化溶剂。为了启动脱保护，核苷衍生物需要经过辐照。这种方法有利于快速、干净、彻底地去除核苷衍生物上的光敏保护基团，从而使合成的核苷酸或寡核苷酸序列达到所需的纯度。
• VERFAHREN ZUR PHOTOLYTISCHEN ABSPALTUNG VON SCHUTZGRUPPEN VON IMMOBILISIERTEN NUCLEOSID-DERIVATEN, INSBESONDERE IN DER DNA-CHIP-HERSTELLUNG
  申请人：NIGU CHEMIE GMBH

  公开号：EP1159064B1

  公开（公告）日：2004-04-28
• US6552182B2
  申请人：——

  公开号：US6552182B2

  公开（公告）日：2003-04-22