2-[2-(2-{2-[2-(2-Chloroethoxy)-ethoxy]-ethoxy}-ethoxy)-ethyl]-isoindole-1,3-dione

• 分子结构分类: 有机化合物 - 有机杂环化合物 - 异吲哚及其衍生物
• 英文名称: 2-[2-(2-{2-[2-(2-Chloroethoxy)-ethoxy]-ethoxy}-ethoxy)-ethyl]-isoindole-1,3-dione
• 英文别名: 2-[2-[2-[2-[2-(2-chloroethoxy)ethoxy]ethoxy]ethoxy]ethyl]isoindole-1,3-dione
• 化学式: C₁₈H₂₄ClNO₆
• 分子量: 385.8
• InChiKey: OHGFYJPCUJNSCJ-UHFFFAOYSA-N

计算性质

• 辛醇/水分配系数(LogP)：
  1.5
• 重原子数：
  26
• 可旋转键数：
  14
• 环数：
  2.0
• sp3杂化的碳原子比例：
  0.56
• 拓扑面积：
  74.3
• 氢给体数：
  0
• 氢受体数：
  6

反应信息

• 作为产物：
  描述：

  2-[2-(2-{2-[2-(2-Chloroethoxy)-ethoxy}-ethoxyl-ethoxy)-ethoxy]-ethyl}-isoindole-1,3-dione 、 2-(2-{2-[2-(2-chloroethoxy)-ethoxy]-ethoxy}-ethyl)-isoindole-1,3-dione 、 生成 2-[2-(2-{2-[2-(2-Chloroethoxy)-ethoxy]-ethoxy}-ethoxy)-ethyl]-isoindole-1,3-dione
  参考文献：
  名称：

  METHOD OF IMMOBILIZING A PROTEIN OR MOLECULE VIA A MUTANT DEHALOGENASE THAT IS BOUND TO AN IMMOBILIZED DEHALOGENASE SUBSTRATE AND LINKED DIRECTLY OR INDIRECTLY TO THE PROTEIN OR MOLECULE

  摘要：

  提供了一种突变的水解酶，可选择性地与感兴趣的蛋白质融合。该突变的水解酶能够与相应的非突变（野生型）水解酶的底物形成键合，其比野生型水解酶和底物之间形成的键合更加稳定，并且与野生型水解酶相比至少有两个氨基酸替换。还提供了包含一个或多个功能基团的水解酶底物，以及使用该突变水解酶和本发明的底物的方法。还提供了一种融合蛋白，能够与底物形成稳定的键合，并且表达该融合蛋白的细胞。

  公开号：

  US20120330001A1

文献信息

• COVALENT TETHERING OF FUNCTIONAL GROUPS TO PROTEINS
  申请人：PROMEGA CORPORATION

  公开号：US20160031911A1

  公开（公告）日：2016-02-04

  A mutant hydrolase optionally fused to a protein of interest is provided. The mutant hydrolase is capable of forming a bond with a substrate for the corresponding nonmutant (wild-type) hydrolase which is more stable than the bond formed between the wild-type hydrolase and the substrate. Substrates for hydrolases comprising one or more functional groups are also provided, as well as methods of using the mutant hydrolase and the substrates of the invention. Also provided is a fusion protein capable of forming a stable bond with a substrate and cells which express the fusion protein.

  提供了一个可选地与感兴趣的蛋白质融合的突变水解酶。该突变水解酶能够与对应的非突变（野生型）水解酶的底物形成比野生型水解酶与底物形成的键更稳定的键。还提供了包含一个或多个功能基团的水解酶底物，以及使用所述突变水解酶和底物的方法。还提供了一个能够与底物形成稳定键的融合蛋白质，以及表达该融合蛋白质的细胞。
• Covalent tethering of functional groups to proteins
  申请人：——

  公开号：US20040214258A1

  公开（公告）日：2004-10-28

  A mutant hydrolase optionally fused to a protein of interest is provided. The mutant hydrolase is capable of forming a bond with a substrate for the corresponding nonmutant (wild-type) hydrolase which is more stable than the bond formed between the wild-type hydrolase and the substrate. Substrates for hydrolases comprising one or more functional groups are also provided, as well as methods of using the mutant hydrolase and the substrates of the invention. Also provided is a fusion protein capable of forming a stable bond with a substrate and cells which express the fusion protein.

  提供了一种可选地与感兴趣的蛋白质融合的突变水解酶。该突变水解酶能够与相应的非突变（野生型）水解酶的底物形成比野生型水解酶和底物形成的键更稳定的键。还提供了包含一个或多个功能基团的水解酶底物，以及使用该突变水解酶和本发明的底物的方法。还提供了能够与底物形成稳定键的融合蛋白质和表达该融合蛋白质的细胞。
• Substrates for covalent tethering to proteins
  申请人：Darzins Aldis

  公开号：US20050272114A1

  公开（公告）日：2005-12-08

  A mutant hydrolase optionally fused to a protein of interest is provided. The mutant hydrolase is capable of forming a bond with a substrate for the corresponding nonmutant (wild-type) hydrolase which is more stable than the bond formed between the wild-type hydrolase and the substrate and has at least two amino acid substitutions relative to the wild-type hydrolase. Substrates for hydrolases comprising one or more functional groups are also provided, as well as methods of using the mutant hydrolase and the substrates of the invention. Also provided is a fusion protein capable of forming a stable bond with a substrate and cells which express the fusion protein.

  提供了一种可选地与感兴趣的蛋白质融合的突变水解酶。该突变水解酶能够与相应的非突变（野生型）水解酶的底物形成更稳定的键合作用，而且相对于野生型水解酶至少有两个氨基酸替换。还提供了含有一个或多个功能基团的水解酶底物，以及使用该突变水解酶和本发明的底物的方法。还提供了能够与底物形成稳定键合作用的融合蛋白质以及表达该融合蛋白质的细胞。
• Covalent tethering of functional groups to proteins and substrates therefor
  申请人：Darzins Aldis

  公开号：US20080274488A1

  公开（公告）日：2008-11-06

  A mutant hydrolase optionally fused to a protein of interest is provided. The mutant hydrolase is capable of forming a bond with a substrate for the corresponding nonmutant (wild-type) hydrolase which is more stable than the bond formed between the wild-type hydrolase and the substrate and has at least two amino acid substitutions relative to the wild-type hydrolase. Substrates for hydrolases comprising one or more functional groups are also provided, as well as methods of using the mutant hydrolase and the substrates of the invention. Also provided is a fusion protein capable of forming a stable bond with a substrate and cells which express the fusion protein.

  提供了一种可选地与感兴趣的蛋白质融合的突变水解酶。该突变水解酶能够与相应的非突变（野生型）水解酶的底物形成键合，该键合比野生型水解酶与底物形成的键合更稳定，并且相对于野生型水解酶至少有两个氨基酸替换。还提供了包含一个或多个功能基团的水解酶的底物，以及使用该突变水解酶和本发明的底物的方法。还提供了一种能够与底物形成稳定键合的融合蛋白和表达该融合蛋白的细胞。
• Method of immobilizing a protein or molecule via a mutant dehalogenase that is bound to an immobilized dehalogenase substrate and linked directly or indirectly to the protein or molecule
  申请人：Darzins Aldis

  公开号：US20090098627A1

  公开（公告）日：2009-04-16

  A mutant hydrolase optionally fused to a protein of interest is provided. The mutant hydrolase is capable of forming a bond with a substrate for the corresponding nonmutant (wild-type) hydrolase which is more stable than the bond formed between the wild-type hydrolase and the substrate and has at least two amino acid substitutions relative to the wild-type hydrolase. Substrates for hydrolases comprising one or more functional groups are also provided, as well as methods of using the mutant hydrolase and the substrates of the invention. Also provided is a fusion protein capable of forming a stable bond with a substrate and cells which express the fusion protein.

  本发明提供了一种可选地与感兴趣的蛋白质融合的突变水解酶。该突变水解酶能够与相应的非突变（野生型）水解酶的底物形成比野生型水解酶和底物形成的键更稳定的键，并且相对于野生型水解酶至少有两个氨基酸置换。还提供了包含一个或多个功能基团的水解酶底物，以及使用该突变水解酶和本发明的底物的方法。还提供了一种能够与底物形成稳定键的融合蛋白质和表达该融合蛋白质的细胞。