5-trifluoroacetamidoallyl-2'-deoxyuridine

• 分子结构分类: 有机化合物 - 核苷、核苷酸和类似物 - 嘧啶核苷
• 英文名称: 5-trifluoroacetamidoallyl-2'-deoxyuridine
• 英文别名: 2,2,2-trifluoro-N-[1-[1-[(2R,4S,5R)-4-hydroxy-5-(hydroxymethyl)oxolan-2-yl]-2,4-dioxopyrimidin-5-yl]prop-2-enyl]acetamide
• 化学式: C₁₄H₁₆F₃N₃O₆
• 分子量: 379.293
• InChiKey: MAKPRXWTOJYQTG-HCZOVWHVSA-N

计算性质

• 辛醇/水分配系数(LogP)：
  0.1
• 重原子数：
  26
• 可旋转键数：
  5
• 环数：
  2.0
• sp3杂化的碳原子比例：
  0.5
• 拓扑面积：
  128
• 氢给体数：
  4
• 氢受体数：
  9

反应信息

• 作为产物：
  描述：

  K2 PdCl4 、 N-烯丙基-2,2,2-三氟乙酰胺 、 硫化氢 、 5-(chloromercuri)-2'-deoxyuridine 生成 5-trifluoroacetamidoallyl-2'-deoxyuridine
  参考文献：
  名称：

  摘要：

  DOI：

文献信息

• Fast and specific immobilization of nucleic acids to solid supports
  申请人：MOLECULAR DIAGNOSTICS, INC.

  公开号：EP0192197A2

  公开（公告）日：1986-08-27

  A process for synthesizing an oligonucleotide comprising linking a nucleoside phosphate to a solid support, through the heterocyclic moiety of the nucleoside, coupling a mono-or oligonucleotide to the nucleoside phosphate through its phosphate moiety, in at least one step enzymatically lengthening the mono- or oligonucleotide, cleaving the resultant oligonucleotide from the solid support-nucleoside phosphate at the phosphate moiety of the nucleoside, and separating the oligonucleotide. After cleaving and separating the solid support-nucleoside phosphate is recycled for further coupling. Advantageously the solid support-nucleoside phosphate is phosphorylated between separation and recycling.

  一种合成寡核苷酸的工艺，包括通过核苷的杂环分子将核苷磷酸酯连接到固体支持物上，通过核苷磷酸酯的磷酸分子将单核或寡核苷酸偶联到核苷磷酸酯上、在至少一个步骤中酶切延长单核苷酸或寡核苷酸，在核苷酸的磷酸基上从固体支持物-核苷酸磷酸酯上裂解产生的寡核苷酸，并分离寡核苷酸。在裂解和分离后，固体支持物-核苷磷酸酯被回收用于进一步偶联。有利的是，在分离和回收之间，固体支持物-核苷磷酸会被磷酸化。
• Labelling of oligonucleotides
  申请人：MOLECULAR DIAGNOSTICS, INC.

  公开号：EP0194545A2

  公开（公告）日：1986-09-17

  A method is provided of making an oligonucleotide which comprises hybridizing (a) a shorter fragment of the desired oligonucleotide with (b) a nucleic acid fragment longer than - (a) and complementary to the desired oligonucleotide, one of (a) and (b) carrying an organic radical which differentiates its physical properties relative to the other of (a) and (b), contacting the hybridized material with an enzyme and nucleoside triphosphates whereby the shorter fragment grows in one direction until it is substantially cotermined with the complementary nucleic acid fragment, denaturing the hybridized material and separating lengthened (a) from (b).

  提供了一种制造寡核苷酸的方法，该方法包括将(a)所需寡核苷酸的较短片段与(b)长于(a)并与所需寡核苷酸互补的核酸片段杂交，(a)和(b)中的一个带有有机基，该有机基使其相对于(a)和(b)中的另一个具有不同的物理特性、将杂交材料与酶和三磷酸核苷接触，使较短的片段向一个方向生长，直到与互补的核酸片段基本同源，使杂交材料变性，将延长的（a）与（b）分离。
• Specific iodination of nucleic acid
  申请人：MOLECULAR DIAGNOSTICS, INC.

  公开号：EP0198207A1

  公开（公告）日：1986-10-22

  A probe comprising a nucleic acid fragment covalently linked to an iodinatable residue. Such nucleic acid probes can be readily radiolabeled such as by prelabeling the nucleic acid with a nonradioactive iodinatable residue, and then reacting with radioactive iodine.

  一种探针，由与可碘化残基共价连接的核酸片段组成。 这种核酸探针很容易进行放射性标记，例如用非放射性可碘化残基对核酸进行预标记，然后与放射性碘反应。
• Prolonged chemiluminescence
  申请人：MILES INC.

  公开号：EP0210449B1

  公开（公告）日：1993-07-28
• US4808520A
  申请人：——

  公开号：US4808520A

  公开（公告）日：1989-02-28