申请人:Board of Regents, The University of Texas System
公开号:US10228355B2
公开(公告)日:2019-03-12
There is provided a system for performing a chromatographic separation of an analyte, methods of using the system to separate at least one component of an analyte and an eluent generator of use in the system. An exemplary system comprises: (a) an eluent generator comprising: (i) a housing configured to be pressurizable by gas, comprising an annular void defined by the housing, and a gas inlet for the gas and a gas outlet for the gas in fluid communication with the annular void; (ii) a membrane permeable to the gas defining an eluent flow channel disposed within the annular void, the eluent flow channel having an eluent precursor fluid inlet and an eluent outlet; (iii) a source of gas in fluidic communication with the gas inlet; (iv) a source of the eluent precursor fluid; and (b) a chromatography column disposed downstream of and in fluidic communication with the eluent outlet.
本发明提供了一种用于对分析物进行色谱分离的系统、使用该系统分离分析物中至少一种组分的方法以及在该系统中使用的洗脱液发生器。一个示例性系统包括: (a) 洗脱液发生器,包括(i) 可被气体加压的壳体,包括由壳体限定的环形空隙,以及与环形空隙流体相通的气体入口和气体出口;(ii) 可渗透气体的膜,该膜限定了设置在环形空隙内的洗脱液流道,该洗脱液流道具有洗脱液前驱体流体入口和洗脱液出口;(iii) 与气体入口流体连通的气体源; (iv) 洗脱前体流体源;以及 (b) 设置在洗脱液出口下游并与洗脱液出口流体连通的色谱柱。