2'-脱氧-6-硫代鸟苷5'-三磷酸酯 | 17660-38-7

• 分子结构分类: 有机化合物 - 核苷、核苷酸和类似物 - 嘌呤核苷酸
• 中文名称: 2'-脱氧-6-硫代鸟苷5'-三磷酸酯
• 英文名称: 2'-Deoxy-6-thioguanosine 5'-triphosphate
• 英文别名: [[(2R,3S,5R)-5-(2-amino-6-sulfanylidene-3H-purin-9-yl)-3-hydroxyoxolan-2-yl]methoxy-hydroxyphosphoryl] phosphono hydrogen phosphate
• CAS: 17660-38-7
• 化学式: C₁₀H₁₆N₅O₁₂P₃S
• 分子量: 523.25
• InChiKey: BXZRFHUVVWIHMV-KVQBGUIXSA-N

计算性质

• 辛醇/水分配系数(LogP)：
  -4.6
• 重原子数：
  31
• 可旋转键数：
  8
• 环数：
  3.0
• sp3杂化的碳原子比例：
  0.5
• 拓扑面积：
  290
• 氢给体数：
  7
• 氢受体数：
  14

反应信息

• 作为产物：
  描述：

  6-硫鸟嘌呤 在 E_. coli hypoxanthine phosphoribosyl transferase 、 Meiothermus ruber polyphosphate kinase D₁₂₇S 、 Thermus virus ribonucleotide reductase P₇₄-23 、 magnesium chloride 、 1,4-二巯基-2,3-丁二醇 作用下， 以 aq. buffer 为溶剂， 反应 2.5h， 生成 2'-脱氧-6-硫代鸟苷5'-三磷酸酯
  参考文献：
  名称：

  10.1002/cctc.202400181

  摘要：

  AbstractPolyphosphate kinases (PPKs) are valuable biocatalysts for ATP cofactor regeneration as well as for the phosphorylation of non‐canonical nucleotides. The versatility of PPKs in the latter application is defined by their substrate scope. In this study, we investigate the substrate spectrum of the PPK2‐III enzyme from Meiothermus ruber (MrPPK) for the conversion of canonical and non‐canonical (deoxy)‐ribonucleotides. The enzyme shows high substrate promiscuity for purine and to minor degree pyrimidine substrates, with an overall preference for the native substrate AMP. With structure guided rational amino acid exchanges in the active site, we produced an MrPPK variant with improved activity for a broad variety of purine nucleotides. While the preference for AMP is lost, conversion of nucleotides without a 6‐amino function at the purine moiety is increased. This MrPPK variant is a versatile biocatalyst for the synthesis of non‐canonical nucleotides and could also be useful as a GTP cofactor regeneration system.

  DOI：

  10.1002/cctc.202400181

文献信息

• [EN] PHOSPHATE AND PHOSPHONATE BASED COMPOUNDS OF 6-THIO-2'-DEOXYGUANOSINE AS ANTI-CANCER AGENTS<br/>[FR] COMPOSÉS À BASE DE PHOSPHATE ET DE PHOSPHONATE DE 6-THIO -2'-DÉSOXYGUANOSINE COMME AGENTS ANTICANCÉREUX
  申请人：MINGHUI PHARMACEUTICAL (SHANGHAI) LTD

  公开号：WO2020154917A1

  公开（公告）日：2020-08-06

  Phosphates and phosphonates compounds of 6-thio-2'-deoxyguanosine are provided. The uses and pharmaceutical compositions of these compounds are disclosed.

  提供了6-硫-2'-脱氧鸟苷的磷酸盐和膦酸盐化合物。这些化合物的用途和药用组合物已被披露。
• Sample preparation method
  申请人：Oxford Nanopore Technologies Ltd.

  公开号：US10669578B2

  公开（公告）日：2020-06-02

  The invention relates to an improved method for characterising a template polynucleotide. The method involves using a polymerase to prepare a modified polynucleotide which makes it easier to characterise than the template polynucleotide.

  本发明涉及一种表征模板多核苷酸的改进方法。该方法包括使用聚合酶制备修饰的多核苷酸，使其比模板多核苷酸更容易表征。
• Methods and compositions for diagnosis and treatment of cancer based on the transcription factor ets2
  申请人：MUSC Foundation For Research Development.

  公开号：US20020081601A1

  公开（公告）日：2002-06-27

  The present invention relates to methods for treating and preventing cancer by modifying the expression of ets2 gene expression or the activity of the gene product. The invention also relates to sensitizing cancer cells to chemotherapeutic or radiotherapeutic agents. Ets2 gene expression and/or activity of the gene product can be modulated using antisense ets2 nucleic acids and/or modified ets2 proteins. The present invention also provides pharmaceutical compositions which comprise antisense ets2 nucleic acid, and nucleic acid that encode modified ets2 proteins and/or modified ets2 proteins.

  本发明涉及通过改变 ets2 基因表达或基因产物活性来治疗和预防癌症的方法。本发明还涉及使癌细胞对化疗或放射治疗药物敏感的方法。可以使用反义 ets2 核酸和/或修饰的 ets2 蛋白调节 Ets2 基因的表达和/或基因产物的活性。本发明还提供了包含反义 ets2 核酸和编码修饰的 ets2 蛋白和/或修饰的 ets2 蛋白的核酸的药物组合物。
• METHODS AND COMPOSITIONS FOR DIAGNOSIS AND TREATMENT OF CANCER BASED ON THE TRANSCRIPTION FACTOR ETS2
  申请人：MUSC Foundation For Research Development

  公开号：EP1133575A2

  公开（公告）日：2001-09-19
• EP1133575A4
  申请人：——

  公开号：EP1133575A4

  公开（公告）日：2003-08-13