2,4,5,7-四溴-3,6-二羟基-3-氧代-3H-螺[异苯并呋喃-1,9-氧杂蒽]-6-羧酸 | 3561-24-8

• 分子结构分类: 有机化合物 - 有机杂环化合物 - 苯并吡喃
• 中文名称: 2,4,5,7-四溴-3,6-二羟基-3-氧代-3H-螺[异苯并呋喃-1,9-氧杂蒽]-6-羧酸
• 英文名称: 2',4',5',7'-Tetrabromo-3',6'-dihydroxy-3-oxo-3H-spiro[isobenzofuran-1,9'-xanthene]-6-carboxylic acid
• 英文别名: 2',4',5',7'-tetrabromo-3',6'-dihydroxy-1-oxospiro[2-benzofuran-3,9'-xanthene]-5-carboxylic acid
• CAS: 3561-24-8
• 化学式: C₂₁H₈Br₄O₇
• 分子量: 691.9
• InChiKey: FPDSBVLLOWTCAO-UHFFFAOYSA-N

计算性质

• 辛醇/水分配系数(LogP)：
  5.7
• 重原子数：
  32
• 可旋转键数：
  1
• 环数：
  5.0
• sp3杂化的碳原子比例：
  0.05
• 拓扑面积：
  113
• 氢给体数：
  3
• 氢受体数：
  7

文献信息

• COMPOUNDS HAVING ENERGY TRANSFER FUNCTION AND METHOD FOR DNA BASE SEQUENCING BY USING THE SAME
  申请人：THE INSTITUTE OF PHYSICAL & CHEMICAL RESEARCH

  公开号：EP0967219A1

  公开（公告）日：1999-12-29

  Disclosed are compounds having two kinds of reporters that can be a donor and an acceptor for energy transfer, for example, fluorescent groups, and having a 2',3'-dideoxyribonucleotide residue or a 3'-deoxyribonucleotide residue. These compounds can be used as terminators for the chain terminator method. The two kinds of reporters are arranged with a distance sufficient for causing energy transfer from the donor to the acceptor. Also disclosed are methods for determining DNA sequences based on the chain terminator method wherein the chain termination reaction is performed by using the above terminators. Also disclosed are compounds having two kinds of reporters that can be a donor and an acceptor for energy transfer, which can be used as a primer or an initiator in methods for determining DNA sequences utilizing the chain terminator method, and methods for determining DNA sequences utilizing the compounds.

  所公开的化合物具有两种可作为能量转移供体和受体的报告体，例如荧光基团，并具有 2',3'-二脱氧核糖核苷酸残基或 3'-脱氧核糖核苷酸残基。这些化合物可用作链终止器方法的终止剂。这两种报告基因的排列距离足以使能量从供体转移到受体。还公开了基于链终止器法确定 DNA 序列的方法，其中链终止反应是通过使用上述终止子进行的。还公开了具有两种报告基因的化合物，它们可以是能量转移的供体和受体，在利用链终止器法确定DNA序列的方法中可用作引物或引发剂，以及利用这些化合物确定DNA序列的方法。