A test composition comprising glucose oxidase, a peroxidatively active component, a chromogenic indicator system capable of providing a detectable response and a borate buffer capable of providing an initial pH above about pH 7 is particularly useful for the semiquantitative determination of high range glucose, (i.e. glucose concentrations of 1,000 mg/dL to 10,000 mg/dL). A preferred indicator system is a water soluble iodide salt and poly-(vinylpyrrolidone). The use of a borate buffer capable of providing an initial pH above about pH 7 permits greatly improved resolution for the semiquantitative determination of high range glucose when the test composition is incorporated onto a carrier matrix to prepare a solid state unitary test device.
由
葡萄糖氧化酶、过氧化活性成分、能提供可检测反应的显色指示剂系统和能提供高于约 pH7 初始 pH 值的
硼酸盐缓冲液组成的测试组合物特别适用于高范围
葡萄糖的半定量测定(即
葡萄糖浓度为 1,000 mg/dL 至 10,000 mg/dL)。首选的指示剂系统是
水溶性
碘盐和聚(
乙烯基吡咯烷酮)。使用
硼酸盐缓冲液可使初始 pH 值高于约 pH 值 7,当测试组合物加入载体基质以制备固态单元测试装置时,可大大提高高范围
葡萄糖半定量测定的分辨率。