A novel fluorescent group-containing carbodiimide compound having at least one group selected from a carboxyl group, a sulfo group, a phosphono group and a phospho group which have substitution of an alkali metal, an alkaline earth metal or a basic group containing a nitrogen or phosphorus atom, which is prepared by using a novel fluorescent group-containing carbodiimide compound precursor having a halogen atom or a sulfonic acid group; and a method for detecting a nucleic acid by hybridization utilizing a nucleic acid labeled with a labeling substance, which utilizes the fluorescent group-containing carbodiimide compound as the labeling substance.
一种新型含荧光基团碳二
亚胺化合物,其至少具有一个选自羧基、磺基、膦基和膦基的基团,这些基团具有碱
金属、碱土
金属或含有氮原子或
磷原子的碱性基团的取代基,该化合物是通过使用具有卤素原子或
磺酸基的新型含荧光基团碳二
亚胺化合物前体制备的;以及一种利用用标记物质标记的核酸通过杂交检测核酸的方法,该方法利用含荧光基团的碳二
亚胺化合物作为标记物质。