| 1616382-72-9

• 分子结构分类: 有机化合物 - 有机聚合物 - 多肽
• CAS: 1616382-72-9
• 化学式: C₅₄H₈₅AuN₁₆O₁₈SSe
• 分子量: 1554.36
• InChiKey: RBZZJSHWIMVKHX-OTHJUVLMSA-M

反应信息

• 作为产物：
  描述：

  [di-(1,3-diethylbenzimidazol-2-ylidene)]gold(I) 、 Ac-NH-SGGDILQSGC-selenocystine-G-NH₂ 在 水 作用下， 以 乙腈 为溶剂， 反应 0.5h， 生成 、
  参考文献：
  名称：

  Insights on the mechanism of thioredoxin reductase inhibition by Gold N-heterocyclic carbene compounds using the synthetic linear Selenocysteine containing C-terminal peptide hTrxR(488-499): An ESI-MS investigation

  摘要：

  Gold-based drugs typically behave as strong inhibitors of the enzyme thioredoxin reductase (hTrxR), possibly as the consequence of direct Gold(I) coordination to its active site selenocysteine. To gain a deeper insight into the molecular basis of enzyme inhibition and prove gold-selenocysteine coordination, the reactions of three parent Gold(I) NHC compounds with the synthetic C-terminal dodecapeptide of hTrxR containing Selenocysteine at position 498, were investigated by electrospray ionization mass spectrometry (ESI-MS). Formation of 1:1 Gold-peptide adducts, though in highly different amounts, was demonstrated in all cases. In these adducts the same [Au-NHC](+) moiety is always associated to the intact peptide. Afterward, tandem MS experiments, conducted on a specific Gold-peptide complex, pointed out that Gold is coordinated to the selenolate group. The relatively large strength of the Gold-selenolate coordinative bond well accounts for potent enzyme inhibition typically afforded by these Gold(I) compounds. In a selected case, the time course of enzyme inhibition was explored. Interestingly, enzyme inhibition turned out to show up very quickly and reached its maximum just few minutes after mixing. Overall, the present results offer some clear insight into the process of thioredoxin reductase inhibition by Gold-based compounds.

  DOI：

  10.1016/j.jinorgbio.2014.01.009