4-(氨基甲基)-3',6'-二羟基-3H-螺[2-苯并呋喃-1,9'-氧杂蒽]-3-酮盐酸盐(1:1) | 91539-64-9

• 分子结构分类: 有机化合物 - 有机杂环化合物 - 苯并吡喃
• 中文名称: 4-(氨基甲基)-3',6'-二羟基-3H-螺[2-苯并呋喃-1,9'-氧杂蒽]-3-酮盐酸盐(1:1)
• 中文别名: 4’-胺甲基荧光素盐酸盐；4-(氨基甲基)-3',6'-二羟基-3H-螺[2-苯并呋喃-1,9'-氧杂蒽]-3-酮盐酸盐
• 英文名称: aminomethylfluorescein hydrochloride
• 英文别名: 4-(Aminomethyl)-3',6'-dihydroxy-3H-spiro[isobenzofuran-1,9'-xanthen]-3-one hydrochloride；7-(aminomethyl)-3',6'-dihydroxyspiro[2-benzofuran-3,9'-xanthene]-1-one;hydrochloride
• CAS: 91539-64-9
• 化学式: C₂₁H₁₅NO₅*ClH
• 分子量: 397.815
• InChiKey: IWNKTMSFMCEAEO-UHFFFAOYSA-N

计算性质

• 辛醇/水分配系数(LogP)：
  3.55
• 重原子数：
  28
• 可旋转键数：
  1
• 环数：
  5.0
• sp3杂化的碳原子比例：
  0.1
• 拓扑面积：
  102
• 氢给体数：
  4
• 氢受体数：
  6

反应信息

• 作为反应物：
  描述：

  4-(氨基甲基)-3',6'-二羟基-3H-螺[2-苯并呋喃-1,9'-氧杂蒽]-3-酮盐酸盐(1:1) 、 伍德沃德氏试剂K 在 三乙胺 、 三氟乙酸 作用下， 以 二氯甲烷 、 N,N-二甲基甲酰胺 为溶剂， 生成 去甲替林
  参考文献：
  名称：

  Reagents and methods for the quantification of amitriptyline or

  摘要：

  披露了用于在测试样本中特异定量氨曲南或诺曲南的免疫测定方法和试剂，采用经氨曲南或诺曲南衍生物制备的抗体。其中对于氨曲南，R为CH3，对于诺曲南，R为H。本发明还描述了结构为Formula IV和Formula V的独特荧光示踪剂的合成。对于特定的氨曲南免疫测定，W1是连接到芳香环的2或3位置的杂原子，对于特定的诺曲南免疫测定，W2是两个连接在一起并附在芳香环的2或3位置的杂原子，其中Q是可检测的基团，优选为荧光素或荧光素衍生物。

  公开号：

  US05614419A1

文献信息

• Methadone fluorescence polarization immunoassay
  申请人：Abbott Laboratories

  公开号：US05073629A1

  公开（公告）日：1991-12-17

  A fluorescence polarization immunoassay for detecting the presence of methadone in a test sample is provided, based upon competition between methadone and a fluorescently labeled tracer for the binding sites on an antibody specific for methadone. The concentration of methadone in the sample determines the amount of tracer that binds to the antibody. The amount of tracer-antibody complex formed can be quantitatively measured and is inversely proportional to the quantity of methadone in the sample. Tracers used as reagents and immunogens used to raise antibodies for use as reagents are also disclosed.

  提供了一种用于检测测试样本中甲庚酮存在的荧光偏振免疫分析方法，该方法基于甲庚酮与荧光标记示踪剂在特异于甲庚酮的抗体结合位点上的竞争。样本中甲庚酮的浓度决定了与抗体结合的示踪剂的数量。形成的示踪剂-抗体复合物的数量可以定量测量，并且与样本中甲庚酮的数量成反比。还公开了用作试剂的示踪剂和用于培养用作试剂的抗体的免疫原。
• Phenylacetylglutamine (PAG) analytical test
  申请人：Abbott Laboratories

  公开号：US05100807A1

  公开（公告）日：1992-03-31

  The present invention is directed to a fluorescence polarization immunoassay for determining the phenylacetylglutamine (PAG) content in body fluids, to the various components needed for preparing and carrying out such an assay, and to the methods of making these components. Specifically, tracers, immunogens and antibodies are disclosed, as well as methods for preparing them. The assay is conducted by measuring the degree of polarization of plane polarized light that has been passed through a solution continuing sample, antiserum and tracer.

  本发明涉及一种用于测定体液中苯乙酰谷氨酰胺（PAG）含量的荧光偏振免疫分析方法，涉及准备和执行此类分析所需的各种组分，以及制备这些组分的方法。具体包括揭示了示踪剂、免疫原和抗体，以及制备它们的方法。该分析通过测量透过含有样品、抗血清和示踪剂的溶液的偏振平面光的偏振度来进行。
• Reagents, methods and kits for an amphetamine-class fluorescence
  申请人：Abbott Laboratories

  公开号：US05248791A1

  公开（公告）日：1993-09-28

  A fluorescence polarization immunoassay (FPIA) for detecting the presence of one or more amphetamine-class analytes in a test sample is provided. The immunoassay uses competition between the analyte and a fluorescently labeled tracer for the binding site on an antibody specific for phenethylamine derivatives. The concentration of amphetamine-class analyte in the sample determines the amount of tracer that binds to the antibody. The amount of tracer/antibody complex formed can be quantitatively measured and is inversely proportional to the quantity of analyte in the test sample. The invention relates to tracers, to immunogens used to elicit antibodies for use as assay reagents, and to assay kits incorporating these tracers and assay reagents.

  提供了一种用于检测测试样本中存在的一种或多种苯丙胺类分析物的荧光偏振免疫分析（FPIA）。该免疫分析利用分析物与一种荧光标记的示踪剂在特异于苯乙胺衍生物的抗体的结合位点上的竞争。样本中苯丙胺类分析物的浓度决定了结合到抗体的示踪剂的数量。形成的示踪剂/抗体复合物的数量可以定量测量，并且与测试样本中分析物的数量成反比。该发明涉及用于诱导用作测定试剂的抗体的免疫原和包含这些示踪剂和测定试剂的测定试剂盒。
• 4'-aminomethyfluorescein derivatives for use in fluorescence polarization immunoassys
  申请人：ABBOTT LABORATORIES

  公开号：EP0201751A2

  公开（公告）日：1986-11-20

  The present invention is directed to an improved method for the preparation of 4'-aminomethylfluorescein derivatives for use in fluorescence polarization immunoassays. The invention is also directed to a novel class of 4'-N-alkylaminomethylfluorescein derivatives possessing utility as reagents in such assays.

  本发明涉及一种用于荧光偏振免疫测定的 4'-氨甲基荧光素衍生物的改进制备方法。本发明还涉及一类新型的 4'-N-烷基氨甲基荧光素衍生物，该类衍生物在此类检测中可用作试剂。
• 3-methoxy-4-hydroxyphenylglycol fluorescence polarization immunoassay
  申请人：ABBOTT LABORATORIES

  公开号：EP0252405A2

  公开（公告）日：1988-01-13

  The present invention is directed to a fluorescence polarization immunoassay for determining the 3-methoxy-4-hydroxyphenylglycol content in body fluids, to the various components needed for preparing and carrying out such an assay, and to methods of making these components. Specifically, tracers, immunogens and antibodies are disclosed, as well as methods for preparing them. The assay is conducted by measuring the degree of polarization of plane polarized light that has been passed through a sample containing antiserum and tracer.

  本发明涉及一种测定体液中 3-甲氧基-4-羟基苯乙二醇含量的荧光偏振免疫分析法，涉及制备和进行这种分析法所需的各种成分，以及制备这些成分的方法。 具体地说，本发明公开了示踪剂、免疫原和抗体，以及制备它们的方法。 该分析法是通过测量穿过含有抗血清和示踪剂的样品的平面偏振光的偏振程度来进行的。