2'-N-Glycyl-2'-amino-2'-deoxyuridin | 43176-70-1

• 分子结构分类: 有机化合物 - 核苷、核苷酸和类似物 - 嘧啶核苷
• 英文名称: 2'-N-Glycyl-2'-amino-2'-deoxyuridin
• 英文别名: 2'-glycylamino-2'-deoxy-uridine；2-amino-N-[(2R,3R,4S,5R)-2-(2,4-dioxopyrimidin-1-yl)-4-hydroxy-5-(hydroxymethyl)oxolan-3-yl]acetamide
• CAS: 43176-70-1
• 化学式: C₁₁H₁₆N₄O₆
• 分子量: 300.271
• InChiKey: XLCJDTVPUFRJLH-UUOKUNOPSA-N

计算性质

• 辛醇/水分配系数(LogP)：
  -2.9
• 重原子数：
  21
• 可旋转键数：
  4
• 环数：
  2.0
• sp3杂化的碳原子比例：
  0.55
• 拓扑面积：
  154
• 氢给体数：
  5
• 氢受体数：
  7

文献信息

• Solid phase sequencing of double-stranded nucleic acids
  申请人：——

  公开号：US20030096258A1

  公开（公告）日：2003-05-22

  Methods for detecting and sequencing of target double-stranded nucleic acid molecules, nucleic acid probes and arrays of probes useful in these methods, and kits and systems that contain these probes aer provided. The methods involve hybridizing the nucleic acids or nucleic acids which represent complementary or homologous sequences of the target to an array of nucleic acid probes. These probes include a single-stranded portion, an optional double-stranded portion and a variable sequence within the single-stranded portion. The molecular weights of the hybridized nucleic acids of the set can be determined by mass spectroscopy, and the sequence of the target determined from the molecular weights of the fragments.

  提供了用于检测和测序目标双链核酸分子的方法，用于这些方法的核酸探针和探针阵列，以及包含这些探针的试剂盒和系统。这些方法涉及将与目标互补或同源序列相对应的核酸或核酸的杂交到核酸探针阵列上。这些探针包括单链部分、可选的双链部分和单链部分内的可变序列。可以通过质谱法确定一组杂交核酸的分子量，并从碎片的分子量确定目标的序列。
• SOLID PHASE SEQUENCING OF BIOPOLYMERS
  申请人：Cantor Charles

  公开号：US20110172111A1

  公开（公告）日：2011-07-14

  This invention relates to methods for detecting and sequencing target nucleic acid sequences, to mass modified nucleic acid probes and arrays of probes useful in these methods, and to kits and systems which contain these probes. Useful methods involve hybridizing the nucleic acids or nucleic acids which represent complementary or homologous sequences of the target to an array of nucleic acid probes. These probes comprise a single-stranded portion, an optional double-stranded portion and a variable sequence within the single-stranded portion. The molecular weights of the hybridized nucleic acids of the set can be determined by mass spectroscopy, and the sequence of the target determined from the molecular weights of the fragments. Nucleic acids whose sequences can be determined include DNA or RNA in biological samples such as patient biopsies and environmental samples. Probes may be fixed to a solid support such as a hybridization chip to facilitate automated molecular weight analysis and identification of the target sequence.

  本发明涉及检测和测序目标核酸序列的方法，以及在这些方法中有用的质量修饰核酸探针和探针阵列，以及包含这些探针的试剂盒和系统。有用的方法涉及将代表目标互补或同源序列的核酸或核酸杂交到核酸探针阵列上。这些探针包括单链部分，可选的双链部分和单链部分内的可变序列。通过质谱法确定集合的杂交核酸的分子量，并从碎片的分子量确定目标的序列。可以确定序列的核酸包括生物样本中的DNA或RNA，例如患者活检和环境样本。探针可以固定在固体支持上，例如杂交芯片上，以便于自动化的分子量分析和目标序列的鉴定。
• Dna sequencing by mass spectrometry
  申请人：SEQUENOM, INC.

  公开号：EP1262564A2

  公开（公告）日：2002-12-04

  The invention describes a new method to sequence DNA. The improvements over the existing DNA sequencing technologies are high speed, high throughput, no electrophoresis and gel reading artifacts due to the complete absence of an electrophoretic step, and no costly reagents involving various substitutions with stable isotopes. The invention utilizes the Sanger sequencing strategy and assembles the sequence information by analysis of the nested fragments obtained by base-specific chain termination via their different molecular masses using mass spectrometry, as for example, MALDI or ES mass spectrometry. A further increase in throughput can be obtained by introducing mass-modifications in the oligonucleotide primer, chain-terminating nucleoside triphosphates and/or in the chain-elongating nucleoside triphosphates, as well as using integrated tag sequences which allow multiplexing by hybridization of tag specific probes with mass-differentiated molecular weights.

  本发明描述了一种新的 DNA 测序方法。与现有的 DNA 测序技术相比，本发明的改进之处在于速度快、通量高，由于完全没有电泳步骤，因此不会产生电泳和凝胶读取假象，也不需要用稳定同位素进行各种替换的昂贵试剂。本发明利用桑格测序策略，通过使用质谱（如 MALDI 或 ES 质谱）分析碱基特异性链终止得到的嵌套片段的不同分子质量，从而组装序列信息。通过在寡核苷酸引物、链终止核苷三磷酸酯和/或链延长核苷三磷酸酯中引入质量修饰，以及使用集成标记序列，通过与具有不同分子量的标记特异性探针杂交，可进一步提高通量。
• NOVEL DEOXYRIBONUCLEOSIDE PHOSPHORAMIDITES AND THEIR USE FOR THE PREPARATION OF OLIGONUCLEOTIDES
  申请人：CALIFORNIA INSTITUTE OF TECHNOLOGY

  公开号：EP0270651A1

  公开（公告）日：1988-06-15
• EP0270651A4
  申请人：——

  公开号：EP0270651A4

  公开（公告）日：1989-03-29