| 1402843-86-0

• 分子结构分类: 有机化合物 - 核苷、核苷酸和类似物 - 嘧啶核苷
• CAS: 1402843-86-0
• 化学式: C₂₁H₃₁N₇O₁₅P₂
• 分子量: 683.463
• InChiKey: XXSOPJFFLCHRQH-CZRWPLIISA-N

计算性质

• 辛醇/水分配系数(LogP)：
  -3.97
• 重原子数：
  45.0
• 可旋转键数：
  13.0
• 环数：
  4.0
• sp3杂化的碳原子比例：
  0.62
• 拓扑面积：
  326.72
• 氢给体数：
  7.0
• 氢受体数：
  16.0

反应信息

• 作为产物：
  描述：

  在 ribonuclease P 作用下， 反应 0.08h， 生成 、 2'-脱氧腺苷-5'-单磷酸
  参考文献：
  名称：

  A Single Nuclease-Resistant Linkage in DNA as a Versatile Tool for the Characterization of DNA Lesions: Application to the Guanine Oxidative Lesion “G+34” Generated by Metalloporphyrin/KHSO₅ Reagent

  摘要：

  The oxidation of an oligonucleotide containing a single nuclease-resistant phosphodiester link, a stereoisomerically pure methylphosphonate, by manganese (Mn-TMPyP) or iron (Fe-TMPyP) porphyrin associated to KHSO5 allowed the isolation and characterization of a guanine lesion corresponding to an increase of mass of 34 amu as compared to guanine ("G+34"), namely, 5-carboxamido-5-formamido-2-iminohydantoin. Enzymatic digestion of the damaged oligonucleotide afforded, apart from the undamaged nucleotide monomer pool, a unique dinudeotide doubly modified with a methylphosphonate and an oxidized guanine base that is suitable for NMR analysis. The method can be applied to the study of any DNA lesion. More importantly, the method can be extended to the analysis of DNA damage in a sequence context. Any preselected residue in a DNA sequence may be individually analyzed by the easy introduction of a single nuclease-resistant link at the 3'- or 5'-position.

  DOI：

  10.1021/tx300319y