2-Amino-6-hydroxy-8-thiocyanato-purin | 19447-80-4

• 分子结构分类: 有机化合物 - 有机杂环化合物 - 咪唑并嘧啶类
• 英文名称: 2-Amino-6-hydroxy-8-thiocyanato-purin
• 英文别名: 2-amino-8-thiocyanato-1,7(9)-dihydro-purin-6-one；(2-Amino-6-oxo-1,7-dihydropurin-8-yl) thiocyanate
• CAS: 19447-80-4
• 化学式: C₆H₄N₆OS
• 分子量: 208.203
• InChiKey: XMNYVEUNOUNDRW-UHFFFAOYSA-N

计算性质

• 辛醇/水分配系数(LogP)：
  -0.2
• 重原子数：
  14
• 可旋转键数：
  1
• 环数：
  2.0
• sp3杂化的碳原子比例：
  0.0
• 拓扑面积：
  145
• 氢给体数：
  3
• 氢受体数：
  5

文献信息

• Pseudonucleotide comprising an intercalator
  申请人：Christensen Bech Ulf

  公开号：US20060014144A1

  公开（公告）日：2006-01-19

  The present invention relates to intercalator pseudonucleotides. Intercalator pseudonucleotides according to the invention are capable of being incorporated into the backbone of a nucleic acid or nucleic acid analogue and they comprise an intercalator comprising a flat conjugated system capable of co-stacking with nucleobases of DNA. The invention also relates to oligonucleotides or oligonucleotide analogues comprising at least one intercalator pseudo nucleotide. The invention furthermore relates to methods of synthesising intercalator pseudo nucleotides and methods of synthesising oligonucleotides or oligonucleotide analogues comprising at least one intercalator pseudonucleotide. In addtition, the invention describes methods of separating sequence specific DNA(s) from a mixture comprising nucleic acids, methods of detecting a sequence specific DNA (target DNA) in a mixture comprising nucleic acids and/or nucleic acid analogues and methods of detecting a sequence specific RNA in a mixture comprising nucleic acids and/or nucleic acid analogues. In particular said methods may involve the use of oligonucleotides comprising intercalator pseudo nucleotides. The invention furthermore relates to pairs of oligonucleotides or oligonucleotide analogues capable of hybridising to one another, wherein said pairs comprise at least one intercalator pseudonucleotide. Methods for inhibiting a DNAse and/or a RNAse and methods of modulating transcription of one or more specific genes are also described.

  本发明涉及插入剂假核苷酸。本发明中的插入剂假核苷酸能够被合并到核酸或核酸类似物的骨架中，它们包括一个插入剂，该插入剂包括一个平面共轭系统，能够与DNA的核碱基共同堆积。本发明还涉及至少包括一个插入剂假核苷酸的寡核苷酸或寡核苷酸类似物。此外，本发明还涉及合成插入剂假核苷酸的方法以及合成至少包括一个插入剂假核苷酸的寡核苷酸或寡核苷酸类似物的方法。此外，本发明还描述了一种从包含核酸的混合物中分离特定序列DNA的方法，检测包含核酸和/或核酸类似物的混合物中的特定序列DNA（目标DNA）的方法以及检测包含核酸和/或核酸类似物的混合物中的特定序列RNA的方法。特别是，这些方法可能涉及使用包括插入剂假核苷酸的寡核苷酸。本发明还涉及能够杂交的寡核苷酸或寡核苷酸类似物的对，其中所述对包括至少一个插入剂假核苷酸。本发明还描述了抑制DNA酶和/或RNA酶的方法以及调节一个或多个特定基因的转录的方法。
• PSEUDONUCLEOTIDE COMPRISING AN INTERCALATOR
  申请人：Christensen Ulf Bech

  公开号：US20100121056A1

  公开（公告）日：2010-05-13

  The present invention relates to intercalator pseudonucleotides. Intercalator pseudonucleotides according to the invention are capable of being incorporated into the backbone of a nucleic acid or nucleic acid analogue and they comprise an intercalator comprising a flat conjugated system capable of co-stacking with nucleobases of DNA. The invention also relates to oligonucleotides or oligonucleotide analogues comprising at least one intercalator pseudo nucleotide. The invention furthermore relates to methods of synthesising intercalator pseudo nucleotides and methods of synthesising oligonucleotides or oligonucleotide analogues comprising at least one intercalator pseudonucleotide. In addition, the invention describes methods of separating sequence specific DNA(s) from a mixture comprising nucleic acids, methods of detecting a sequence specific DNA (target DNA) in a mixture comprising nucleic acids and/or nucleic acid analogues and methods of detecting a sequence specific RNA in a mixture comprising nucleic acids and/or nucleic acid analogues. In particular said methods may involve the use of oligonucleotides comprising intercalator pseudo nucleotides. The invention furthermore relates to pairs of oligonucleotides or oligonucleotide analogues capable of hybridising to one another, wherein said pairs comprise at least one intercalator pseudonucleotide. Methods for inhibiting a DNAse and/or a RNAse and methods of modulating transcription of one or more specific genes are also described.

  本发明涉及插入剂伪核苷酸。本发明的插入剂伪核苷酸能够被合并到核酸或核酸类似物的骨架中，并且它们包括一个插入剂，该插入剂包含一个平面共轭系统，能够与DNA的碱基共堆叠。本发明还涉及至少包括一个插入剂伪核苷酸的寡核苷酸或寡核苷酸类似物。此外，本发明还涉及合成插入剂伪核苷酸的方法以及合成至少包括一个插入剂伪核苷酸的寡核苷酸或寡核苷酸类似物的方法。此外，本发明还描述了从包括核酸的混合物中分离序列特异性DNA的方法，检测包括核酸和/或核酸类似物的混合物中的序列特异性DNA（目标DNA）的方法，以及检测包括核酸和/或核酸类似物的混合物中的序列特异性RNA的方法。特别是所述方法可能涉及使用包括插入剂伪核苷酸的寡核苷酸。本发明还涉及能够杂交到一起的寡核苷酸或寡核苷酸类似物的配对，其中所述配对包括至少一个插入剂伪核苷酸。还描述了抑制DNA酶和/或RNA酶的方法以及调节一个或多个特定基因的转录的方法。
• THERAPEUTIC AGENT, TREATMENT METHOD, PROPHYLACTIC AGENT, AND PROPHYLACTIC METHOD FOR CANINE AND FELINE IMMUNOLOGICAL DISEASES
  申请人：TORAY INDUSTRIES, INC.

  公开号：EP0919241A1

  公开（公告）日：1999-06-02

  The present invention discloses a remedy, preventive agent, treatment method and preventive method for immune diseases of dogs and cats. As an active ingredient of the remedy and preventive agent, canine interleukin 12 is used. Specifically, the present invention relates to an immune disease remedy and preventive agent of dogs and cats comprising canine interleukin 12 of a heterodimer formed by an amino acid sequence identical with or having a part of Sequence number:1 or Sequence number:11 and an amino acid sequence identical with or having a part of Sequence number:2 or Sequence number:12. Furthermore, the present invention relates to an immune disease treating method and preventive method for dogs and cats which is characterized in injecting the immune disease remedy and preventive agent of dogs and cats into a dog or cat.

  本发明公开了一种治疗猫狗免疫疾病的药方、预防剂、治疗方法和预防方法。作为治疗和预防剂的有效成分，使用了犬白细胞介素 12。 具体而言，本发明涉及一种犬和猫的免疫疾病治疗和预防剂，它包含由与序列号：1 或序列号：11 相同或具有其中一部分的氨基酸序列和与序列号：2 或序列号：12 相同或具有其中一部分的氨基酸序列形成的异二聚体犬白细胞介素 12。此外，本发明涉及一种狗和猫的免疫疾病治疗方法和预防方法，其特征在于将狗和猫的免疫疾病治疗剂和预防剂注射到狗或猫体内。
• Device and method for high-throughput quantification of mRNA from whole blood
  申请人：Hitachi Chemical Company, Ltd.

  公开号：EP2058405A1

  公开（公告）日：2009-05-13

  Disclosed are a method, device kit, and automated system for simple, re-producible, and high-throughput quantification of mRNA from whole blood. More particularly, the method, device, kit and automated system involve combinations of leukocyte filters attached to oligo(dT)-immobilized multi-well plates.

  本发明公开了一种用于简单、可重复和高通量量化全血 mRNA 的方法、装置套件和自动系统。更具体地说，该方法、装置、试剂盒和自动化系统涉及连接到寡聚（dT）固定多孔板的白细胞过滤器组合。
• One step sample preparation and detection of nucleic acids in complex biological samples
  申请人：——

  公开号：US20030039974A1

  公开（公告）日：2003-02-27

  A method for simultaneous release and detection of nucleic acids from complex biological samples is described. The invention relates to the combined use of lysis buffers containing strong chaotropic agents such as guanidine thiocyanate to facilitate cell lysis and release of cellular nucleic acids and to the use of a novel type of bicyclic nucleotide analogues, locked nucleic acid (LNA) to detect specific nucleic acids released during lysis by nucleic acid hybridisation. In particular methods are described for the covalent attachment of the catching LNA-oligo. Novel methods for sample preparation of e.g. polyadenylated mRNA species are also presented. The invention further addresses reagents for performing the methods as well as reagents and applications of the method.

  本发明描述了一种从复杂生物样品中同时释放和检测核酸的方法。本发明涉及结合使用含有硫氰酸胍等强致乱剂的裂解缓冲液来促进细胞裂解和细胞核酸的释放，以及使用新型双环核苷酸类似物--锁定核酸（LNA）来通过核酸杂交检测裂解过程中释放的特定核酸。特别介绍了捕获 LNA-oligo 的共价连接方法。本发明还介绍了用于制备多腺苷酸 mRNA 等样品的新方法。本发明进一步论述了执行这些方法的试剂以及试剂和方法的应用。