磷酸萘酚AS-MX | 1596-56-1

• 物质功能分类: 化学试剂 - 有机试剂 - 多环化合物
• 分子结构分类: 有机化合物 - 苯类化合物 - 萘
• 中文名称: 磷酸萘酚AS-MX
• 中文别名: 萘酚AS-MX磷酸盐；色酚AS-MX磷酸盐
• 英文名称: naphthol AS-MX phosphate
• 英文别名: [3-[(2,4-dimethylphenyl)carbamoyl]naphthalen-2-yl] dihydrogen phosphate
• CAS: 1596-56-1
• 化学式: C₁₉H₁₈NO₅P
• mdl: MFCD00042711
• 分子量: 371.329
• InChiKey: IOMLBTHPCVDRHM-UHFFFAOYSA-N

物化性质

• 熔点：
  189-190°C
• 密度：
  1.419±0.06 g/cm3(Predicted)
• 稳定性/保质期：
  在常温常压下稳定，避免氧化物接触。

计算性质

• 辛醇/水分配系数(LogP)：
  3.1
• 重原子数：
  26
• 可旋转键数：
  4
• 环数：
  3.0
• sp3杂化的碳原子比例：
  0.105
• 拓扑面积：
  95.9
• 氢给体数：
  3
• 氢受体数：
  5

安全信息

• 危险品标志：
  Xi
• 危险类别码：
  R36/37/38
• WGK Germany：
  3
• 安全说明：
  S26,S36
• 危险标志：
  GHS07
• 危险性描述：
  H315,H319,H335
• 危险性防范说明：
  P261,P305 + P351 + P338
• 储存条件：
  2-8°C条件下请密封保存。

反应信息

• 作为产物：
  描述：

  2-羟基-3-萘甲酸 在 五氯化磷 、 三氯化磷 作用下， 以 1,4-二氧六环 为溶剂， 反应 3.17h， 生成 磷酸萘酚AS-MX
  参考文献：
  名称：

  Fluorescent Substrates for Potential Use in Enzyme-Linked Immunosorbent Assay of Membrane-Bound Nucleic Acids

  摘要：

  A number of phosphorylated fluorochromes were synthesized and tested for the alkaline phosphatase-linked fluorescence assay of membrane-bound nucleic acids. 3-Hydroxy-N-2'-biphenyl-2-naphthalenecarboxamide phosphate ester (HBNP) was found to be the most suitable for the assay. Nonfluorescent HBNP is hydrolyzed by phospholipase bound to the probe DNA to the highly fluorescent 3-hydroxy-N,2'-biphenyl-2-naphthalenecarboxamide (HBN). HBN is insoluble enough in aqueous medium to precipitate on the nylon membrane. Spots containing as little as 5 fg of lambda DNA can be successfully detected on the membrane with HBNP.

  DOI：

  10.1021/ac00080a022

文献信息

• METHODS AND COMPOSITIONS FOR ENZYME LINKED IMMUNO AND HYBRIDIZATION MASS SPECTROMETRIC ASSAY
  申请人：YYZ Pharmatech, Inc.

  公开号：US20160223530A1

  公开（公告）日：2016-08-04

  Disclosed are sensitive methods for detecting a target substance in low concentrations. The method can include immobilizing a target substance to a solid phase; incubating the immobilized target substance with a reporter enzyme detection probe in solution under conditions for forming target: enzyme detection probe complexes; washing the solid phase to remove any unbound reporter enzyme detection probe; incubating the target: enzyme detection probe complex with a reporter enzyme detection probe substrate in substrate reaction solution to generate one or more ionizable products; and detecting one or more of the one or more ionizable products using mass spectrometry (MS).

  本发明涉及一种敏感的低浓度目标物检测方法。该方法可以包括将目标物质固定到固相上；在形成目标物：酶检测探针复合物的条件下，将固定的目标物质与溶液中的报告酶检测探针孵育；洗涤固相以去除任何未结合的报告酶检测探针；将目标物：酶检测探针复合物与报告酶检测探针底物在底物反应溶液中孵育，以产生一个或多个可离子化产物；并使用质谱（MS）检测一个或多个可离子化产物中的一个或多个。
• Method and device for the assay of alkaline phosphatase in dairy products
  申请人：ADVANCED INSTRUMENTS, INC.

  公开号：EP0401813A2

  公开（公告）日：1990-12-12

  A method and device for the assay of alkaline phosphatase in dairy products. A sample of the dairy product is added to a self-­indicating fluorometric substrate comprising a single orthophosphoric ester on a phenolic ring. After hydrolysis of the phosphate radical by the alkaline phosphatase in the sample, the substrate and the sample added thereto are exposed to electromagnetic radiation at a first wavelength. The increase in fluorescence at a second wavelength produced by the hydrolysis of the phosphate radical by the alkaline phosphatase is determined. This increase is linearly related to the activity of alkaline phosphatase present in the sample of the dairy product.

  一种检测乳制品中碱性磷酸酶的方法和装置。将乳制品样品添加到由酚醛环上的单个正磷酸酯组成的自指示荧光测定底物中。样品中的碱性磷酸酶水解磷酸自由基后，将基质和加入其中的样品置于第一波长的电磁辐射下。测定碱性磷酸酶水解磷酸基所产生的第二波长荧光增加量。这种增加与乳制品样品中存在的碱性磷酸酶的活性成线性关系。
• REAGENT SYSTEM AND METHOD
  申请人：Leica Biosystems Melbourne Pty Ltd

  公开号：EP2459983B1

  公开（公告）日：2018-01-17
• IMMUNOASSAY REAGENTS AND METHODS OF USE THEREOF
  申请人：BIOCARE MEDICAL, LLC.

  公开号：US20140057803A1

  公开（公告）日：2014-02-27

  The present invention provide reagents and methods of using the reagents, for example, on automated staining devices, that facilitate detection of two or more antigens in a sample simply and efficiently.
• US5790627A
  申请人：——

  公开号：US5790627A

  公开（公告）日：1998-08-04